Method for cultivating plant tissue under non-aseptic condition

A plant tissue culture, non-sterile technology, applied in the field of plant tissue culture, can solve the problems of long seedling hardening period, low survival rate, high cost, and achieve the effect of less investment and superior growth.

Inactive Publication Date: 2005-06-22
单文修
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] In order to solve the problems of high investment, high risk, long seedling hardening period, low survival rate and high cost caused by microbial contamination in plant tissue culture, in order to make this biotechnology popular in China, especially for the general public Used by farmers, the inventors have proposed a method for culturing plant tissue under natural conditions close to the natural growth of crops, that is, under non-sterile natural environmental conditions without any strict disinfection and sterilization treatment

Method used

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  • Method for cultivating plant tissue under non-aseptic condition
  • Method for cultivating plant tissue under non-aseptic condition
  • Method for cultivating plant tissue under non-aseptic condition

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Experimental program
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Embodiment Construction

[0135] The specific implementation manners and effects of the present invention are now introduced in a tabular manner.

[0136] 1: Preparation of antibacterial medium

[0137] There are many types of medium used in plant tissue culture, such as MS, VW, WH, N6, WS, etc. Among them, MS medium is the most widely used. The preparation process of MS medium is as follows:

[0138] 1. Prepare working mother liquor. The macroelements and microelements are prepared into 100 times the working mother solution of the required concentration. As shown in Table 5

[0139] Mother liquor number

Drug Name

content in g

1

ammonium nitrate

Potassium dihydrogen phosphate

boric acid

Manganese sulfate

Zinc sulfate

potassium iodide

Sodium molybdate

165

17

0.62

2.23

0.86

0.083

0.025

copper sulfate

Cobalt chloride

0.0025

0.0025

2

potassium nitrate

190

3

calcium chlo...

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Abstract

The invention provides a method for cultivating plant tissue under non-aseptic condition, wherein the whole procedure of plant tissue cultivation is conducted under the natural ambient condition of non-asepsis, the method consists of adding disinfection and bactericidal agent into the culture media, preparing bacterium suppression culture media, inoculating under the natural ambient condition of non-asepsis, and placing the culture material in natural ambient condition meeting plant tissue culture.

Description

Technical field: [0001] The invention belongs to plant tissue culture technology in the field of biotechnology, in particular to a plant tissue culture method under non-sterile natural environment conditions. Background technique: [0002] Plant tissue culture is a technique developed on the basis of plant physiology at the beginning of this century. It is the application of sterile techniques to cultivate an isolated organ, tissue or cell of a plant. After more than 80 years of hard work by scientists from various countries, almost all plants have successfully achieved the in vitro culture of their organs, tissues and cells. Most also regenerate countless plantlets from parts of cells, or even bare protoplasts without cell walls. [0003] Plant tissue culture technology has become one of the most important and active fields in agricultural high-tech. It is not only the basis for the continued development of agriculture, but also the most widely used and most practical fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A61L2/16
Inventor 单文修
Owner 单文修
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