Method for cultivating tissue cultured gloden line lotus seedling

A culturing method and a technique for tissue culture seedlings, which are applied in the field of tissue culture seedling cultivation of Clematis clematis, can solve problems such as the application of hormone-free culture seedlings of test-tube seedlings, which has not been reported, and are suitable for large-scale production, rapid propagation, and safe consumption. Effect

Inactive Publication Date: 2008-02-20
上海雷允上科技发展有限公司 +1
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research about clematis has attracted people's attention. Although there are some researches on tissue culture of clematis, the hormone-free cultivation of test-tube seedlings and the application of its cultivated seedlings have not been reported. In order to ensure the edible quality of clematis cultivated For safety, it is necessary to find a method for hormone-free cultivation of clematis tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the cultivation of clematis regenerated seedlings

[0037] Take the stems or tips of wild clematis as materials, soak them in 70% medical ethanol for about 5 minutes, then transfer them to 10% sodium hypochlorite aqueous solution for disinfection, rinse them with sterile water, and inoculate them in high-temperature sterilized MS+ On the solid medium of NAA0.5mg / L+IBA0.5mg / L+ZT0.2mg / L, the culture conditions are: light (1500lx) 12 hours / day, culture at 25°C, and regenerated buds of Aurea clematis can be obtained after 30 days.

Embodiment 2

[0038] Embodiment 2: Rapid propagation of Clematis clematis tissue culture seedling without hormone

[0039] The regenerated shoots obtained in Example 1 were successively placed on the acclimatization medium with hormone concentration from high to low and then to zero for cultivation.

[0040] The regenerated shoots were inoculated on MS+NAA0.2mg / L+IBA0.2mg / L+ZT0.1mg / L medium for cultivation for 30 days at a cultivation temperature of 20-26°C and light (1500lx) for 12 hours / day.

[0041] Take the above tissue cultured seedlings in MS 0 The culture is carried out on the culture medium for 30 days, the culture temperature is 20-26° C., and the light (1500 lx) is 12 hours / day.

[0042] Finally, fast-growing and robust shoots were selected from the hormone-free medium for subculture (once a month).

Embodiment 3

[0043] Embodiment 3: field cultivation of Clematis clematis tissue culture seedling

[0044] After washing away the culture medium of the root of the tissue culture seedling obtained in Example 2 with clear water, soak it in 0.25% carbendazim solution for 10 minutes and then transplant it into the substrate (loose, air-permeable, moist) with a shade shed to keep growing The temperature is 20-25°C, and the relative humidity is 75-85% in a small environment, and attention should be paid to ventilation in the shed.

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PUM

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Abstract

The invention relates to a method for the plant tissue culture, in particular to the tissue culture method and application of the aoectochilus formosanus. The invention provides a culture method of aoectochilus formosanus tissue culture seedling comprising the steps that: the induction culture is performed by taking the wild aoectochilus formosanus as the raw material, then by means of hormone-free culture, the asepsis culture line of the aoectochilus formosanus tissue culture seedling is obtained; after that, the tissue culture seedling system which is suitable for a large-scale production is established, while the essential effective composition (amylase, amino acid) of the culture is close to the wild aoectochilus formosanus and stable. The main content comprises the configuration of the tissue culture seedling medium; the culture conditions of the tissue culture seedling; and the planting technology and usage of the tissue culture seedling. The invention solves the problem of the lack of natural resources of the wild aoectochilus formosanus.

Description

technical field [0001] The invention relates to a method for culturing plant tissue, in particular to a method for cultivating tissue culture seedlings of Aurantia clematis and its application. Background technique [0002] Clematis is a rare perennial medicinal plant of the family Orchidaceae, and the whole plant can be used as medicine. It has the effects of clearing heat and detoxifying, strengthening the kidney and calming the liver, and lowering blood sugar. Pharmacological studies in recent years have shown that clematis has obvious effects on treating breast cancer. Due to its slow vegetative reproduction and incomplete embryonic development, it is difficult to reproduce in the natural state, resulting in a decrease in the natural resource reserves of wild clematis, and it has become an endangered medicinal plant. The rapid clonal propagation of Clematis clematis using tissue culture technology is the key to solving the shortage of Clematis clematis resources. At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 顾慧芬
Owner 上海雷允上科技发展有限公司
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