Method for inducing protocorms by utilizing dendrobe tissues

Abstract

The invention provides a method for inducing protocorms by utilizing dendrobe tissues. The method comprises the following steps: (1) carrying out routine asepsis on the obtained dendrobe tissues, wherein the dendrobe tissues are from the stem tip, root tip or tender stem section of dendrobe; and (2) carrying out induction culture on the dendrobe tissues subjected to asepsis in an induction culture medium firstly at the temperature of 23-25 DEG C and under a dark condition for one week and then at the temperature of 23-25 DEG C and under an illumination condition for 20-30 days with the illumination period of 12h / day, thus obtaining the protocorms. The method provided by the invention has the following main beneficial effects: (1) the method is simple in operation, low in production cost and less in environmental pollution; (2) the induction success rate is high, wherein the induction rate based on stem section culture is generally about 50%, the induction rate based on root tip culture can reach 60%-70%, and the best induction success rate based on the stem tip culture can reach about 75%; (3) the culture cycle is short, a large number of protocorms can be obtained within less time, and the induced protocorms can be directly subjected to aseptic culture so as to obtain a large number of seedlings; and (4) tissue culture is directly utilized, thus the characters of parents are well maintained.

Description

(1) Technical field [0001] The invention relates to a method for inducing protocorm with dendrobium tissue. (2) Background technology [0002] Dendrobium is one of the largest genera in the orchid family. It is a perennial herb whose origin is mainly distributed in subtropical and tropical regions. There are more than 1,500 species in the world and about 76 species in my country. Dendrobium plants are traditional Chinese medicinal materials in my country. They were first recorded in "Shen Nong's Materia Medica" and in the "Compendium of Materia Medica" by Li Shizhen in the Ming Dynasty. There are about 40 kinds of Dendrobium can be used for medicinal purposes in my country. Among the more famous ones are Dendrobium nobile, Dendrobium officinale and Dendrobium Huoshan. Ancient books record that Dendrobium has the effects of strengthening yin and nourishing essence, nourishing the kidney and strengthening energy, lightening the body and prolonging life. In addition, it is also be...

AI Technical Summary

Problems solved by technology

At present, almost all Dendrobium orchids used as ornamental plants are hybrids, with small seeds, many numbers and incomplete development. The speed is slow, and it is difficult to meet the market's requirements for plant quality. The rapid propagation of Dendrobium by using tissue culture technology can overcome the shortcomings of its seed propagation and branch reproduction technology. Therefore, the research on tissue culture and rapid propagation of Dendrobium is very necessary and has high practical value

Protocorm induction is a key link in tissue culture. At present, the efficiency of protocorm induction in Dendrobium plants is not high, and it has become one of the key steps restricting the rapid reproduction of Dendrobium orchids.

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