Early-flue-cured tobacco quality improving method with eurotium cristatum

A technology of Rhizoctonia coronoidans and tobacco leaves, which is applied to microorganisms to promote the microbial alcoholization (fermentation field of flue-cured tobacco leaves, to achieve the effects of reducing irritation, reducing miscellaneous gas and irritation, and improving taste.

Active Publication Date: 2013-05-22
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of microbial fermentation, there is still the problem of the safety of microbial flavor induction, and the use of microorganisms to improve the internal quality of tobacco leaves, the safety evaluation of tobacco leaves is a problem that cannot be ignored

Method used

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  • Early-flue-cured tobacco quality improving method with eurotium cristatum
  • Early-flue-cured tobacco quality improving method with eurotium cristatum
  • Early-flue-cured tobacco quality improving method with eurotium cristatum

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Embodiment Construction

[0017] Activation culture of Cystis coronaris

[0018] Place the slant strains stored in the refrigerator in an incubator at 30°C for 48 hours, inoculate them on the newly prepared PDA slant medium with an inoculation needle, and cultivate them at 30°C until the colonies cover the slant for later use.

[0019] conidia-producing culture

[0020] 1. M40y medium (conidia medium): 400g sucrose, 20g malt, 5g yeast extract, 20g agar, 1000mL water, natural pH, sterilized at 121°C for 30min;

[0021] 2. Make a plate with M40y medium, use an inoculation needle to pick an appropriate amount of clamshell and inoculate it in the center of the plate, and culture it upside down at 30°C until the plate is covered with colonies;

[0022] 3. The plate produces colonies with yellow surface, lighter color, denser, and gray-green conidia.

[0023] Preparation and inoculation of spore suspension

[0024] Pick activated S. coronis and inoculate it on the M40y medium by spot-patch method, a...

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Abstract

The invention discloses an early-flue-cured tobacco quality improving method with eurotium cristatum. The method comprises the steps that (1) the eurotium cristatum is cultured in an activation mode; (2) conidium bacterial colony culturing is carried out, a conidium bacterial colony culture medium is prepared and is formed into a flat plate, bacterial colonies of bacterial strain slopes from the step (1) are planted on the flat plate in a point mode and are cultured for 5-7 days in an inverting mode until the bacterial colonies overgrow on the flat plate; (3) spore suspension is prepared, conidium generated according to the step (2) is inoculated and is washed off with asepsis water and then is prepared to be the spore suspension which is used as inoculant to be evenly sprayed on early-flue-cured tobacco. When processing conditions are that culturing temperature is 30 DEG C and spore concentration is 106 and 107, aroma of the early-flue-cured tobacco becomes good, impurity odor and irritation are relieved, smoke sweet feeling is improved and is fine, pure and mild, after taste is pure and comfortable, nicotine is lowered, and whole tobacco quality is improved.

Description

Technical field [0001] The present invention involves microbial alcohol (fermentation) method of microorganisms to promote roasting tobacco leaves. Background technique [0002] Tobacco alcohol (fermentation) is an important link to improve the quality of tobacco leaves in tobacco processing. In fact, under a certain temperature and humidity conditions, it has changed the processing process of tobacco leaves, and the aroma and quality of tobacco leaves are significantly improved.Tobacco alcohol (fermentation) mainly has three aspects: oxidation, microbial effects, and tobacco leaves itself.However, the three role contribute to the fermentation of tobacco leaves and the reason for the main reason.However, studies have found that microorganisms are one of the important reasons that promote the fermentation of tobacco alcohol and improve the quality of tobacco leaves. [0003] Chinese scholars use the four superior fungus separated from the tobacco leaves to handle the tobacco leave...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20A24B3/10C12N1/14C12R1/645
Inventor 邹晓瞿娇娇施鸣刘仁祥张晓敏杨辉石志发曾池白兴刘爱英
Owner GUIZHOU UNIV
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