Potato detoxicatubg planting potato asepsis circulating germplasm preserving method

A technology for potatoes and potato seedlings, applied in the field of preservation of potato germplasm resources, can solve the problems of inconvenient popularization and application, short generation time, etc., and achieve the effects of long preservation period, high survival rate and simple operation

Inactive Publication Date: 2008-03-26
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the above two storage methods are: the first storage method requires a liquid nitrogen storage device, which is inconvenient for popularization and application
The second preservation method always needs light, and the subculture time is short and needs to be continuously transferred.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1), use known technology to carry out virus-free culture on the stem tip of the Zhuanxinwu variety of potato seedlings, and obtain the virus-free test tube miniature tubers.

[0017] 2) Cut 10 pieces of sponges with a thickness of 1.0cm and the same size as pads on the bottom of 10 tissue culture bottles, cover the bottle caps, and place the culture bottles at 1.1kg / cm 2 Sterilize under pressure for 30 minutes.

[0018] 3), in the aseptic operation room, transfer the aseptic test tube miniature tubers into the sterile bottles obtained in operation step 2), and each bottle contains 5 test tube miniature tubers.

[0019] 4) Aseptic low-temperature storage of the miniature tubers: store the culture bottle containing the miniature tubers obtained in the operation step 3) in a refrigerator at 3°C.

[0020] 5), generate test-tube seedlings by micro-tubers: after 200 days, take out the culture bottle that test-tube micro-tubers are housed from the refrigerator, inject 20 mL o...

Embodiment 2

[0023] 1) Carry out virus-free culture on the stem tip of the potato seedling Atlantic variety with known technology, and obtain the virus-free test tube miniature tubers.

[0024] 2), cut 2.0cm thick, 10 sponge pads of the same size on the bottom of 10 tissue culture bottles, cover the bottle caps, put the culture bottles at 1.1kg / cm 2 Sterilize under pressure for 40 minutes.

[0025] 3), in the aseptic operation room, transfer the aseptic test tube miniature tubers into the aseptic bottles obtained in the operation step 2), and each bottle contains 10 tubes.

[0026] 4) Aseptic low-temperature preservation of the miniature tubers: store the culture bottle containing the miniature tubers obtained in the filling operation step 3) in a refrigerator at 4°C.

[0027] 5), generate test-tube seedlings by microtubers: after 360 days, take out all culture bottles that test tube microtubers are housed from the refrigerator, and inject 40 mL of sterilized MS liquid medium into each bo...

Embodiment 3

[0030] 1) Virus-free culture of the stem tip of potato seedling cooperation 88 varieties is carried out with known technology, and virus-free test tube miniature tubers are obtained.

[0031] 2) Cut 10 pieces of sponges with a thickness of 1.5cm and place them on the bottom of 10 tissue culture bottles, cover the bottle caps, and place the culture bottles at 1.1kg / cm 2 Sterilize under pressure for 30 minutes.

[0032] 3), in the aseptic operation room, transfer the aseptic test tube miniature tubers into the aseptic bottles obtained in the operation step 2), and each bottle contains 8 pieces.

[0033] 4) Aseptic low-temperature preservation of the miniature tubers: store the culture bottle containing the miniature tubers obtained in the filling operation step 3) in a refrigerator at 5°C.

[0034] 5), generate test-tube seedlings by miniature tubers: after 250 days, take out the culture bottle that test tube miniature tubers are housed from refrigerator, inject 30mL MS liquid ...

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PUM

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Abstract

The present invention relates to a potato detoxified sprout sterile circulation germ plasm preservation method. Said method includes the following steps: using existent tuber tip tissue culture technique to obtain detoxified sprout, utilizing induction process to obtain test tube miniature potato, and it is characterized by that placing said test tube miniature potato into a culture vessel, making low-temperature preservation for 200-360 days at 3-5deg.C, then taking out said test tube miniature potato, under the condition of that the temperature is 20-25deg.C and illumination is 2000-4000 lux making culture for 40-60 days to obtain sterile sprout, under the condition of that temperature is 18-20deg.C culturing said sterile sprout for 60-80 days to obtain miniature potato, repeating above-mentioned operation so as to implement the detoxified potato sprout circulation germ plasm preservation.

Description

technical field [0001] The invention relates to a method for preserving potato germplasm resources, in particular to a method for preserving potato virus-free potato seedlings aseptic circulation germplasm. Background technique [0002] Potato is the fourth largest crop in China and even in the world. Its safe production is related to social stability, and germplasm resources are the basis of agricultural production. Potato is a vegetatively propagated crop, which is susceptible to virus infection during planting and can be passed on in the next year's planting, resulting in the degeneration of the species. Shoot tip tissue culture can effectively detoxify and prevent the degeneration of species, so preserving the vegetative body of detoxified potatoes is the preservation of potato germplasm resources. In the long-term preservation of test-tube plantlets, with the increase and extension of the number of subcultures, the test-tube plantlets have problems such as leggy, thin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01C1/00
Inventor 郭华春王琼龙雯虹蒋从莲
Owner YUNNAN AGRICULTURAL UNIVERSITY
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