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Pleione test tube breeding ball production technique

A production technology and test-tube planting technology, applied in the direction of botany equipment and methods, applications, plant phenotype improvement, etc., can solve the problems of low survival rate, slow reproduction speed, high transportation cost, etc., achieve high survival rate, easy cultivation, high output

Inactive Publication Date: 2005-06-29
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

my country is the main country of origin of the genus Allium genus and has abundant germplasm resources. However, with the change of the ecological environment and the excessive harvesting and smuggling caused by international demand, the wild resources of the genus Allium are seriously damaged.
At present, there is no artificial propagation and cultivation of garlic orchids in China except Taiwan.
[0003] The reproduction of Suanlan orchids is mainly by division, but the propagation speed is slow, and the seeds of Suanlan orchids are extremely difficult to germinate under natural conditions due to incomplete embryo development.
The conventional production of garlic orchids in foreign countries and Taiwan adopts the method of transplanting test-tube seedlings, which has high transportation costs, labor-intensive transplanting and management, and low survival rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Materials: After the Pleione maculata autumn flowers alone garlic orchid bloom, select strong plants to pollinate among different individuals, and take pods to sow 130 days after pollination;

[0015] 2. Pod disinfection: soak the fruit in 75% alcohol for 30 seconds, put it in 0.1% mercuric chloride solution for 20 minutes, rinse it with sterile water for 5 times;

[0016] 3. Seed germination: cut the fruit, inoculate powdered embryos into improved 1 / 2MS medium + 10% coconut milk + 0.5 mg / L of 6-benzylpurine + 0.5 mg / L of naphthalene acetic acid + 2 g / L of activated carbon 1 liter of germination medium; the embryos germinated after about 30 days of culture, and the germination rate was about 60%;

[0017] 4. Seedling cultivation: After continuing to cultivate for about 60 days to form a complete plant, inoculate it on the strong seedling medium of improved Huabao medium + banana 100g / liter + naphthaleneacetic acid 0.5mg / liter for strong seedling culture, and form a s...

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Abstract

The invention relates to a pleione test tube breeding ball production technique, which consists of employing artificial nutrient solution and artificial culture, improving culture temperature, exercising the sprouts at low temperature, obtaining large quantity of tube seed balls and tube sprouts with asepsis sowing method, forming dormancy bulbodium through induction, and taking the bulbodium out of the bottle for convenient transportation, easy cultivation, and high survival rate.

Description

technical field [0001] The invention provides a test-tube bulb production technology of Suanlan orchid. Background technique [0002] There are about 30 species in the genus Orchid, and there are 16 in my country. It has unique shape, bright colors, and high ornamental value, and occupies an important position in the international orchid trade. What's more important is that the solitary orchid can be cultivated and bloomed like bulbous flowers such as hyacinth and lily, which makes it have a very huge development prospect. At present, the international demand for garlic orchids is huge, and the prices in European and American markets are high. However, all wild orchids are protected by international conventions, and their commercial seedlings must be produced through artificial propagation. my country is the main country of origin of the genus Allium genus and has abundant germplasm resources. However, with the change of the ecological environment and the excessive harvesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/02A01H3/04
Inventor 陈之林叶秀麟杨燕仪易琦斐段俊曾宋君梁承邺
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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