Application of Rakicidins compounds in resistance to clinical pathogenic anaerobic bacteria

A technology of compound and anaerobic bacteria, applied in the field of Rakicidins compound, anti-clinical pathogenic anaerobic bacteria, can solve the problem of high recurrence rate

Active Publication Date: 2016-06-29
FUJIAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Anaerobic Clostridium difficile infection is the primary cause of antibiotic-associated diarrhea in hospitals. Metronidazole, vancomycin, and fetamycin are currently recommended drugs for the treatment of Clostridium difficile infection. High relapse rates after either nidazole or vancomycin treatment, urgent need for new therapeutic agents

Method used

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  • Application of Rakicidins compounds in resistance to clinical pathogenic anaerobic bacteria
  • Application of Rakicidins compounds in resistance to clinical pathogenic anaerobic bacteria
  • Application of Rakicidins compounds in resistance to clinical pathogenic anaerobic bacteria

Examples

Experimental program
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Embodiment 1

[0022] Preparation of RakicidinB1

[0023] Step 1: The fermentation and culture conditions of Micromonospora strain FIM02-523 refer to the literature (Jiang Hong, Lin Ru, Zheng Wei, etc. The separation, identification and biological biological activity [J]. Chinese Journal of Antibiotics, 2006, 31(5): 267-270).

[0024] Step 2: Centrifuge the FIM02-523 fermentation broth obtained in Step 1 at 4500rpm for 15 minutes to obtain the mycelium residue, soak the obtained mycelium residue with 2 times the volume of anhydrous methanol or ethanol overnight, and sterilize the bacteria containing alcohol The silk residue was centrifuged again at 4500 rpm for 15 minutes, and the supernatants were combined to obtain a fermentation extract.

[0025] Step 3: HP20 macroporous resin adsorption column chromatography (diameter-to-height ratio 1:5-1:10, column volume 1.5-2.5L): use fermentation extract (40-60L) with 50%-55% Alcohol concentration, the flow rate is 40ml / min for upper column adsorp...

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Abstract

The invention relates to the field of biological medicine, in particular to an application of Rakicidins compounds in resistance to clinical pathogenic anaerobic bacteria. Pharmacodynamic experiments indicate that the Rakicidins compounds have good resistant effect on the clinical pathogenic anaerobic bacteria and have the resistant effect on vancomycin enterococcus infection diseases. The Rakicidins compounds can be used for treating diarrhea, enteritis, alimentary infection, oral infection or skin acne caused by clostridium difficile as well as diseases such as urinary tract infection, or skin soft-tissue infection and the like.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the use of a class of Rakicidins compounds for resisting clinical pathogenic anaerobic bacteria. Background technique [0002] Rakicidin A and B were originally reported in the United States, because their 15-membered cyclic lipopeptide structure contains a rare octyl 4-amino-2,4-pentadienoate and has anti-tumor cell activity [McBrienKD, BerryRL , Lowe SE et al. Rakicidins, New Cytotoxic Lipopeptides from Micromonosporasp. Fermentation, Isolation and Characterization [J]. JAntibiot, 1995, 48: 1446]. The structural formula is as follows: [0003] [0004] Yamazaki (2007) found that RakicidinA has excellent hypoxic selective anti-tumor cell activity, and the anti-colon cancer HCT-8 cell activity under hypoxic conditions is 17.5 times that under normal oxygen conditions [YamazakiY, KunimotoS, IkedaD. RakicidinA: ahypoxia -selectivecytotoxin[J].BiolPharmBull.2007,30(2):261-5.]. Takeu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/12A61P31/04A61P17/10A61P1/12A61P1/02A61P1/00A61P13/00A61P17/00C12N1/20C12P21/04C12R1/29
CPCA61K38/12C12P21/02C12N1/205C12R2001/29
Inventor 林风江红连云阳江宏磊周剑赵薇陈丽方东升
Owner FUJIAN INST OF MICROBIOLOGY
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