Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An application of a saponin thorns in preparing antioxidants

An anti-oxidant, saponin thorn technology, applied in the application field of saponin thorn counterfeit products in the preparation of antioxidants, can solve the problems that have not been studied, and achieve the effect of excellent activity

Active Publication Date: 2021-11-09
SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there is no relevant research or report on the anti-oxidation application of saponins thorn counterfeit products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An application of a saponin thorns in preparing antioxidants
  • An application of a saponin thorns in preparing antioxidants
  • An application of a saponin thorns in preparing antioxidants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The present embodiment measures DPPH free radical scavenging activity by the following method

[0039] (1) Preparation of DPPH solution and test solution

[0040] DPPH · Make 2×10 with absolute ethanol -4 The solution of M was stored at 4°C in the dark. Each monomeric compound and positive drug was made into 1 mg / mL stock solution with absolute ethanol, and diluted with absolute ethanol to the required concentration before testing.

[0041] (2) Determination of activity

[0042] Referring to the experimental method in the literature and improving it, 100 μL of different concentrations of the test solution and 2 × 10 -4 Add 100 μL of MDPPH solution to each well of a 96-well plate, and simultaneously use the concentrations of the test solution without adding DPPH (100 μL absolute ethanol instead of DPPH) as a control to eliminate the interference of the color of the test product itself on the test results. A DPPH·negative control was also set up (100 μL absolute ethan...

Embodiment 2

[0052] The present embodiment measures the oxygen free radical scavenging capacity by the following method

[0053] (1) preparation of reaction reagent and need testing solution

[0054] Fluorescein disodium with 75mM potassium phosphate buffer (75mM KH 2 PO 4 , 75mM K 2 HPO 4 ) to prepare a 63 μM stock solution, store at 4°C in the dark, and dilute 100 times with this buffer solution before testing. AAPH was made into 18.3mM solution with 75mM potassium phosphate buffer before the experiment. Trolox was formulated with 75mM potassium phosphate buffer solution to make a 10μM stock solution, which was diluted to the required concentration before testing. Each monomeric compound and the positive drug VC were made into a 100 mM stock solution with 75 mM potassium phosphate buffer solution, and diluted to the required concentration with the buffer solution before testing.

[0055] (2) Determination of activity

[0056] The ORAC determination used in this experiment refers t...

Embodiment 3

[0063] In the present embodiment, the content of each component of the saponin thorn counterfeit product whose sample number is 1-7 in Table 1 is determined by liquid chromatography

[0064] Agilent ZORBAX SB-Aq C18 (250mm×4.6mm, 5μm) chromatographic column was used, the mobile phase was gradient elution of methanol-0.1% acetic acid solution, the detection wavelength was 254nm, the column temperature was 35°C, and the flow rate was 1.0mL / min. When the volume is 10 μL, the resolution and peak shape of the chromatogram obtained are better, and the number of chromatographic peaks is more and the distribution is uniform. Record the chromatogram to 2 times the retention time (180min), and no chromatographic peak appears after 90min, so the analysis time is 90min, and the peak area is recorded. The results are shown in Table 4 (wherein the sample number corresponds to the sample represented by the sample number in Table 1):

[0065] Table 4

[0066]

[0067]

[0068] It can ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an application of a saponin thorn counterfeit product in the preparation of antioxidants. After the detection of the antioxidant activity of the saponin thorn counterfeit product, compared with rutin, hawthorn extract and other substances, the saponin thorn counterfeit product has It shows comparable activity or even better activity. The DPPH free radical scavenging rate can reach 60%-75%, and the ORAC value can reach 1.20-1.30. It provides a new strategy for the research and development of related preparations and drugs, and has a good application prospects and high application value.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry, and relates to the application of a saponin thorn counterfeit product in the preparation of antioxidants. Background technique [0002] "Chinese Pharmacopoeia" (2015 edition) records that Saponaria thorn has the effects of reducing swelling and detoxification, draining pus, and killing insects; it is used for the initial onset of carbuncle and gangrene or pus does not ulcerate; external treatment of scabies and leprosy. In recent years, with the development and utilization of medicines, the market demand for saponins soars. At present, the quality of the thorns produced by the acacia trees growing in different regions is uneven, and the thorns of many other plants are also mixed in the thorns for sale. Common counterfeit products include Gleditsia japonica Miq., Gleditsiamicrophylla Gordon ex Y.T.Lee, Ziziphus jujuba Mill.var.spinosa (Bunge) Hu ex H.F.Chow, etc., among which It is the most co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/725A61K36/48A61P39/06
CPCA61K36/48A61K36/725A61P39/06
Inventor 殷果王珏王铁杰江坤李菁金一宝王丽君王思明刘凯双
Owner SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com