Eubacterium, Clostridium preparation and use thereof

A technology of Eubacterium and Eubacterium rectum, applied in the field of biopharmaceuticals, can solve the problems such as the inability to produce butyric acid, the unsatisfactory curative effect of the disease, and the impact on the curative effect.

Inactive Publication Date: 2009-08-05
QINGDAO EASTSEA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main ingredients of current microecological preparations include Bifidobacterium, Lactobacillus, Enterococcus, Bacillus licheniformis, Bacillus subtilis, etc., but none of them can produce butyric acid. It needs to be converted into butyric acid to play its role, so the efficacy of treating related diseases is not very ideal
Butyric acid-producing Clostridium butyricum microecological preparations currently sold on the market are still transit bacteria, which cannot colonize the intestinal tract for a long time, and are completely excreted about 4 days after stopping the drug (Yan Shucui, Wan Fuchang, Pu Chunxiao, etc. Clostridium butyric acid Study on the dynamic changes of bacteria in the intestinal tract of mice. Chinese Journal of New Drugs, 2002, 11(4): 325-326), which seriously affected the efficacy of
The biggest feature and originality of the microecological preparation of the present invention is that the selected bacteria are resident beneficial bacteria in the intestinal tract, which can colonize the intestinal tract for a long time and continuously produce butyric acid. Research report, special application for this invention patent

Method used

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  • Eubacterium, Clostridium preparation and use thereof
  • Eubacterium, Clostridium preparation and use thereof
  • Eubacterium, Clostridium preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0063] Medicine Preparation Example 1 Preparation of Oral Rectal Live Bacteria Powder and Cylindrical Live Bacteria Powder

[0064] 1 Preparation of bacterial powder and identification of strains

[0065] Take healthy baby feces, then put the feces in a sterilized anaerobic bottle, blow in nitrogen gas and mix well at the same time, quickly take 2 grams of feces from it and add it to 18mL sterilized diluent, blow in nitrogen gas and mix well at the same time. In the console, do 10 -1 、10 -2 、10 -3 、10 -4 , 10 -5 , 10 -6 , 10 -7 Gradient dilution, take 10 -5 , 10 -6 , 10 -7 Three dilution gradients, spread on the Eubacterium selective single colony isolation solid medium, place in an anaerobic tank, anaerobic culture at 37°C for 48 hours, select two single colonies with good growth and inoculate them into the Eubacterium liquid respectively The expansion medium was placed in an anaerobic tank, and the expansion culture was carried out at 37° C. for 48 hours in anaerob...

preparation Embodiment 2

[0088] Preparation of Oral Clostridium tertiary Live Bacteria Powder and Clostridium Butyricum Live Bacteria Powder Intestinal Residents

[0089] 1 Preparation of bacterial powder and identification of strains

[0090] Take healthy baby feces, then put the feces in a sterilized anaerobic bottle, blow in nitrogen gas and mix well at the same time, quickly take 2 grams of feces from it and add it to 18mL sterilized diluent, blow in nitrogen gas and mix well at the same time. In the console, do 10 -1 、10 -2 、10 -3 、10 -4 , 10 -5 , 10 -6 , 10 -7 Gradient dilution, take 10 -5 , 10 -6 , 10 -7 Three dilution gradients, 80°C water bath for 13 minutes, spread on the solid medium for Clostridium selective single colony isolation, place in an anaerobic tank, culture at 37°C for 48 hours, and select two single colonies with good growth They were respectively inoculated into Clostridium liquid expansion medium, placed in an anaerobic tank, and amplified at 37° C. for 48 hours und...

Embodiment 1

[0112] Example 1. Research on the repairing effect of Eubacterium and Clostridium on intestinal mucosa

[0113] 1 Materials and methods

[0114] 1.1 Materials

[0115] 1.1.1 Drug Rectal Fungal Liquid (10 7 CFU / mL); columnar fungal liquid (10 7 CFU / mL); the third Clostridium solution (10 7 CFU / mL); Intestinal resident Clostridium butyricum liquid (10 7 CFU / mL); Bifidobacteria liquid (10 7 CFU / mL); dextran sodium sulfate (DSS, 10g / bottle, batch number: BI03071, Sino-American Biotec); Alcian blue (1g / bottle, batch number: BS0084, Sino-American Biotec).

[0116] 1.1.2 Animals 70 SD male rats, weighing 80-100 g, standard grade 2, were purchased from the Experimental Animal Center of China Institute for the Control of Pharmaceutical and Biological Products, license number: SCXK (Beijing) 2005-0010.

[0117] 1.2 Method

[0118] 1.2.1 Modeling and treatment of intestinal mucosal injury

[0119] 80 rats, 10 rats were used as the normal control group, and the other 70 rats were ad...

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Abstract

The invention relates to a eubacterium and clostridium preparation and application of the same, in particular to a microecological preparation which is prepared for replenishing butyric acid bacteria and butyric acid produced by intestinal tract by taking single eubacterium, single clostridium or a eubacterium and clostridium composition as a main active composition, and application of the same in treating related diseases through butyric acid production, and belongs to the field of biological medicine.

Description

technical field [0001] The present invention relates to Eubacterium, Clostridium preparation and application thereof, specifically relates to Eubacterium, Clostridium alone or in combination as the main active ingredient to make a microecological preparation supplementing intestinal butyric acid-producing bacteria and butyric acid, and the microecological preparation through The application of producing butyric acid in treating related diseases belongs to the field of biopharmaceuticals. Background technique [0002] Butyric acid (butyric acid) has been proven to be the preferred source of energy for the colon, providing 70% of the energy of the intestinal mucosa, promoting the proliferation and growth of intestinal mucosal cells and the development and maturation of the digestive system. At the same time, butyric acid is also an important substance for regulating immunity. Intestinal butyrate-producing bacteria, therefore, although intestinal butyrate-producing bacteria are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P1/00A61P9/00A61P17/00A61P35/00A61P37/02A61P37/04A61P37/08
Inventor 崔云龙李洪福王发合张海强
Owner QINGDAO EASTSEA PHARMA
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