Lactobacillus plantarum JM113 and application thereof

A technology of Lactobacillus plantarum and JM113, applied to Lactobacillus plantarum JM113 and its application field, can solve the problems of inconsistency and complex probiotic results, small bacteriostatic effect, etc., and achieve the effects of improving production performance and good fermentability

Inactive Publication Date: 2016-05-04
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the existing Lactobacillus reuteri single bacterial agent, Lactobacillus crispatus single bacterial agent and their compound preparations, the content of live bacteria per gram is only 10 7 cfu, the average daily gain of broiler chickens is 50-52g; the high-end lactic acid bacteria microecological preparation developed by Beijing Zhongnong Yingtai Biotechnology Co., Ltd., the product name is Comfort 100, and the daily gain of broiler chickens is 51.97±4.75g after feeding for 42 days; CN201010527172. 6 discloses a strain of Lactobacillus plantarum and its ap

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  • Lactobacillus plantarum JM113 and application thereof
  • Lactobacillus plantarum JM113 and application thereof
  • Lactobacillus plantarum JM113 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the isolation of plantarum lactobacillus JM113

[0027] 1. Strain samples: Tibetan chickens in Tibet are currently mainly raised in traditional free-range methods. They have strong disease resistance and have not been exposed to antibiotics during their growth. They can be used as an ideal source of probiotics. The samples were collected from the intestines of free-range broilers in Yangling, Shaanxi, and the contents of the intestines and mucous membranes were stored at 4°C.

[0028] 2. Culture medium: MRS medium (ManRogosaSharp, MRS), lactobacillus selective medium, the composition is as follows: trypsin 10g / L, yeast extract 5g / L, beef extract 10g / L, sodium acetate trihydrate 5g / L , glucose 20g / L, Tween 801ml / L, dipotassium hydrogen phosphate 2.0g / L, triamine citrate 2.0g / L, magnesium sulfate heptahydrate 0.58g / L, manganese sulfate monohydrate 0.25g / L, calcium carbonate 20g / L, agar 15g / L, distilled water 1L.

[0029] 3. Isolation and purification of ba...

Embodiment 2

[0037] Embodiment 2: the research of acid resistance and bile salt resistance of Lactobacillus plantarum JM113

[0038] 1. First prepare MRS medium with a pH of 2.5 and MRS medium with a bile salt concentration of 0.2%, and sterilize at 121°C for 20 minutes; then pick a single colony of Lactobacillus and add it to 2ml of MRS medium with a bile salt concentration of 0.2% , cultivate overnight at 37°C; then vortex the lactic acid bacteria solution for 30s, take 150μl of the same bacteria solution and transfer it to 4850μl sterilized normal saline, and make a 10-fold gradient dilution with sterilized normal saline (take out 500μl from the first tube and add to 4500μl In sterilized saline, that is, diluted 10 times, each tube must be vortexed for 30 seconds before taking the liquid), and gradually diluted to 10 -6 , take 500 μl to smear on the plate, do 3 repetitions for each bacterium, place the plate in a 37°C incubator for 24 hours, count the number of colonies M after the cult...

Embodiment 3

[0040] Embodiment 3: the research of resistance to trypsin of Lactobacillus plantarum JM113

[0041] (1) Prepare MRS liquid medium with a trypsin concentration of 1 mg / ml (MRS medium is sterilized at high temperature, add trypsin after cooling, dissolve trypsin in sterile water, and filter with a filter membrane)

[0042] (2) Take 150 microliters of the lactic acid bacteria liquid cultured overnight, and after 10-fold gradient dilution with normal saline, smear the plate and count, and record the number of colonies as M; In MRS liquid culture medium, culture at 37°C for 4 hours, and after 10-fold gradient dilution with normal saline, plate the plate and count, and record the number of colonies as N.

[0043] (3) Count the growth rate n=M / N of Lactobacillus plantarum 113

[0044] (4) Experimental results: the initial number of colonies M is 1.83×10 8 CFU

[0045] The number of colonies N=1.61×10 after 4 hours of trypsin treatment 8 CFU

[0046] Survival rate n=88%

[0047...

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Abstract

The invention discloses lactobacillus plantarum JM113, wherein a strain JM113 is preserved in China Center for Type Culture Collection (CCTC) on November 18th, 2015 with a preservation number of CCTC M2015680, and is called simply the JM113. The lactobacillus plantarum JM113 disclosed by the invention can better tolerate factors such as acid and cholate which are unbeneficial to bacterial growth in a gastrointestinal tract and has a good inhibiting action for enteropathogetic bacteria; the lactobacillus plantarum JM113 has better fermentability and potential for mass production. The lactobacillus plantarum JM113 disclosed by the invention is prepared into lactococus lactis powder which is added into broiler diet, so that the production performance of broilers can be remarkably improved.

Description

technical field [0001] The invention relates to a plant lactobacillus JM113 and application thereof. Background technique [0002] With the increasingly prominent disadvantages brought about by the extensive use of antibiotics in animal husbandry, such as the imbalance of normal intestinal flora in animals, the low immunity of animals as a whole, the generation of drug-resistant bacteria and even the residue of antibiotics in livestock and poultry products, It has become an inevitable trend to develop new environmentally friendly, safe and efficient green feed additives to replace antibiotics. [0003] In recent years, probiotics replace antibiotics to promote growth, prevent and treat inflammatory bowel disease has become a research hotspot. Probiotics have broad application prospects in animal husbandry because of their probiotic properties, but not all strains can be used as probiotics. Probiotic strains should have the following characteristics: (1) correct source of pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/75A23K10/18C12R1/25
CPCC12N1/20C12N1/205C12R2001/25
Inventor 杨明明李龙王利红冯军厂赵辛
Owner NORTHWEST A & F UNIV
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