Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mycobacterium tuberculosis antigen protein Rv1808 and application of epitope peptide thereof

A technology of Mycobacterium tuberculosis, rv1808, applied in the direction of bacterial antigen components, peptides, depsipeptides, etc., can solve the problem of few epitopes of Mycobacterium tuberculosis CD8 cells

Inactive Publication Date: 2021-07-27
HELIXGEN GUANGZHOU
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently known tuberculosis CTL epitope peptides are mainly identified in European and American populations, most of which are HLA-A*0201-restricted, but for African and Southeast Asian populations where tuberculosis is most prevalent, there is no suitable epitope peptide to use
HLA-A*2402 is one of the most widely distributed HLA antigens in the world, especially in Asian populations; however, very few HLA-A*2402-specific TB CD8 cell epitopes have been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mycobacterium tuberculosis antigen protein Rv1808 and application of epitope peptide thereof
  • Mycobacterium tuberculosis antigen protein Rv1808 and application of epitope peptide thereof
  • Mycobacterium tuberculosis antigen protein Rv1808 and application of epitope peptide thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Using bioinformatics methods, the inventors studied and analyzed a total of 401 epitope peptides presented by HLA-A*2402 MHC; the 40 epitope peptides with higher scores were analyzed for immunogenicity using Class I Immunogenicity, and selected Four 9mer peptides with higher scores were selected, as shown in Table 1. Net MHC 4.0 was used to analyze the affinity between the four peptides in Table 1 and HLA-A*2402 type MHC, among which Seq ID NO: 1 IPGGWWLTF had the highest affinity (%rank 0.4, BindLevel<=SB).

[0027] Table 1 Basic information of 9mer peptides

[0028]

[0029]

Embodiment 2

[0031] The ELISpot assay detected that the four epitope peptides in Example 1 induced T cells to secrete IFN-γ.

[0032] PBMC cell isolation and cryopreservation: Peripheral blood mononuclear cells (PBMC) of HLA-A*2402 genotype tuberculosis patients were isolated by density gradient method. Dilute the collected 10ml of peripheral blood with PBS at a ratio of 1:1, spread the diluted peripheral blood slowly on top of an equal volume of lymphocyte separation medium, and centrifuge at 800g / min for 30min; use a pipette to carefully absorb buffy coat cells to a new Add 5 times the volume of PBS to wash, centrifuge at 800g / min for 10min; repeat the wash once, freeze the cells with freezing solution (90% FBS+10% DMSO), and store in liquid nitrogen.

[0033] ELISpot experiment: resuscitate PBMCs from patients with HLA-A*2402 genotype, and let them stand overnight in a 5% CO2 incubator at 37 degrees. Remove dead cells from PBMCs with de-dead magnetic beads. For cell counting, resuspen...

Embodiment 3

[0039] MHC tetramer detection of tuberculosis-specific T cells.

[0040] Resuscitate PBMCs of HLA-A*2402(+) genotype tuberculosis patients and HLA-A*2402(-) genotype tuberculosis patients, add IL-2 and PHA to 10% FBS RPMI-1640 medium, and expand PBMCs; HLA-A*2402-MHC IPGGWWLTF Tetramer detection of HLA-A*2402(+) and HLA-A*2402(-) tuberculosis patient PBMC cells; with HLA-A*2402-MHC KFIDTTSKF Tetramer served as an unrelated peptide control.

[0041] MHC tetramer detection of Seq NO1 (IPGGWWLTF) epitope peptide-specific CD8+ T cells: ① Collect PBMC cells by centrifugation, wash twice with PBS, and wash with 2-5×10 7 / ml density resuspended in FACS Buffer (PBS + 2% calf serum + 0.1% sodium azide) to prepare a cell suspension; ②Take 25ul of the cell suspension and drop it into a FACS test tube; ③Prepare 2X Staining Cocktail: FACS Buffer+ MHC tetramer (dilution ratio 1:50~1:100)+Anti-CD8 / FITC antibody (Tonbo, catalog N:35-0086); ④Take 25ul 2x Staining Cocktail, add it dropwise t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology, and particularly relates to a mycobacterium tuberculosis antigen protein Rv1808 and an application of an epitope peptide thereof. A detection reagent prepared from the Rv1808 protein and the epitope peptide thereof can be widely applied to the related fields of auxiliary diagnosis of tuberculosis, epidemiological monitoring and the like, and tuberculosis vaccines and antituberculosis drugs prepared from the Rv1808 protein and the epitope peptide thereof can be applied to prevention and treatment of tuberculosis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a mycobacterium tuberculosis antigenic protein Rv1808 and its epitope peptide. Background technique [0002] Tuberculosis (TB) is an infectious disease mainly caused by respiratory tract infection caused by Mycobacterium Tuberculosis (MTB), and it is the chronic infectious disease with the highest single-cause morbidity rate. According to WHO estimates, about one-third of the world's population is tuberculosis carriers, and about 5-10% of the carriers can develop into active tuberculosis. As one of the countries with a high burden of tuberculosis in the world, China ranks second in the world in the number of tuberculosis patients after India. Therefore, it is urgent to develop tuberculosis vaccines and new detection methods suitable for the Chinese population. [0003] Mycobacterium tuberculosis is an intracellular parasite, and its effective control ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/35G01N33/569C12Q1/02A61K39/04A61P31/06
CPCC07K14/35G01N33/5695G01N33/505A61K39/04A61P31/06G01N2333/35
Inventor 周亮曾庆
Owner HELIXGEN GUANGZHOU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com