7 results about "Mycobacterial antigen" patented technology

The present application relates to the technical field of diagnostic markers, in particular to a marker combination and its application in diagnosing active tuberculosis and distinguishing between latent tuberculosis infection and active tuberculosis. The lectin combination provided by the present application can be used as a marker for ATB diagnosis and distinguishing between LTBI and ATB, and has high specificity and sensitivity. The lectin combination provided by the present application in combination with detection of specific antibodies of mycobacterium tuberculosis antigens can further improve the diagnostic effect. The marker and its detection products provided by the present application have good application potential in ATB diagnosis and distinguishing between LTBI and ATB, and are expected to overcome the limitations of existing diagnostic techniques, improve the diagnostic accuracy and sensitivity of tuberculosis, and provide new ideas and technical means for early detection, precise treatment and effective prevention and control of tuberculosis.

The invention discloses a mycobacterium tuberculosis deletion region protein Rv1577c and a fusion antigen LB28 containing a mycobacterium tuberculosis RD region and a latent infection related antigen, wherein the fusion antigen comprises Rv1577c and Rv1738. The invention provides a candidate antigen Rv1577c of a tuberculosis subunit vaccine through antigen screening and experimental verification. The Rv1577c is deleted in BCG, so that the immunogenicity is better, and the Rv1577c has certain consistency with ESAT6 and CFP10 in bovine tuberculosis immunodiagnosis. And the Rv1577 is combined with a latent infection associated antigen Rv1738 to construct the tuberculosis fusion protein LB28. Under the assistance of an adjuvant DDA + PolyI: C (DP), the fusion protein LB28 shows good immunogenicity in a mouse model, and has an immune protection effect of resisting mycobacterium bovis infection. The Rv1577 and the fusion protein LB28 constructed based on the Rv1577 can be applied to animal and human tuberculosis prevention and treatment.

The present invention relates to compositions and fusion proteins containing at least two Mycobacterium sp. antigens, and polynucleotides encoding such compositions and fusion proteins. The invention also relates to methods for their use in the treatment, prevention and / or diagnosis of tuberculosis infections.

The present application relates to the field of antigen screening, and particularly relates to a mycobacterium tuberculosis antigen screening system, which comprises the following steps: acid-fast staining microscopic images and colony macroscopic images of candidate mutant samples and wild type samples of mycobacterium tuberculosis antigens are input into a shared encoder to generate multi-scale feature maps, wherein the shared encoder comprises a backbone network; the multi-scale feature maps are input into a microscopic decoder to generate mycobacterium features, wherein the microscopic decoder is a lightweight U-Net architecture; the multi-scale feature maps are input into a macroscopic decoder to generate colony texture features, wherein the macroscopic decoder comprises an ASPP layer and a colony texture output head; immunological verification mutant samples are screened based on feature distribution differences of the mycobacterium features and the colony texture features of the candidate mutant samples and the wild type samples. The visual recognition algorithm of the present application utilizes the correlation information of the microscopic mycobacterium features and the macroscopic colony texture features of the mycobacterium tuberculosis antigens, and realizes comprehensive antigen primary screening batch screening.

The present invention provides a method of detecting antibodies in a sample from a subject, wherein the antibodies bind to epitopes of Mycobacterium tuberculosis antigens, comprising contacting the sample with two or more isolated polypeptides or antigenic fragments or variants thereof, wherein the polypeptides comprise polypeptides selected from the group consisting of SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13, and 15; and detecting formation of antibody-peptide complexes comprising said isolated polypeptides or antigenic fragments or variants thereof, wherein formation of said complexes is indicative of the presence of the antibodies to epitopes of Mycobacterium tuberculosis antigens in said sample.

This invention relates to the field of in vitro diagnostic technology and provides a highly specific Mycobacterium tuberculosis antigen detection kit and detection method. The kit includes: a thermosensitive antigen aggregate composed of two or more of ESAT-6, CFP-10, and MPT-64 coupled with PNIPAM-b-PLL block copolymer, which spontaneously forms nanoscale antigen clusters at 37°C; dual-mechanism anti-fouling magnetic beads, which are Fe3O4@SiO2@PEG-polysulfobetaine three-layer core-shell structures, with a reference fluorescent dye embedded in the SiO₂ layer via covalent bonding, and the surface covalently coupled to the thermosensitive antigen aggregate via carboxyl groups; an AIE fluorescent probe, composed of AIE fluorescent microspheres coupled with an anti-tuberculosis antigen detection antibody; and a sample diluent. This invention achieves local enrichment of antigen through temperature-controlled aggregation, minimizes background through dual-mechanism anti-fouling magnetic beads (PEG steric hindrance + zwitterionic super hydration), and amplifies the signal and performs internal reference calibration through AIE ratio fluorescence. The three components work synergistically to achieve high sensitivity, low background, strong anti-interference ability, and easy operation for tuberculosis antigen detection.

The invention relates to the technical field of diagnostic markers, in particular to a marker combination and application thereof in diagnosing active tuberculosis and distinguishing latent tuberculosis infection and active tuberculosis. The lectin combination provided by the invention can be used as a marker for ATB diagnosis and distinguishing of LTBI and ATB, and has relatively high specificity and sensitivity. The lectin combination provided by the invention is combined with the mycobacterium tuberculosis antigen to detect the specific antibody, so that the diagnosis effect can be further improved. The marker and the detection product thereof provided by the invention have relatively good application potential in ATB diagnosis and LTBI and ATB distinguishing, and are expected to overcome the limitation of the existing diagnosis technology, improve the diagnosis accuracy and sensitivity of tuberculosis, and provide a new thought and technical means for early discovery, precise treatment and effective prevention and control of tuberculosis.