3 results about "Mycobacterial antigen" patented technology

The present application relates to the technical field of diagnostic markers, in particular to a marker combination and its application in diagnosing active tuberculosis and distinguishing between latent tuberculosis infection and active tuberculosis. The lectin combination provided by the present application can be used as a marker for ATB diagnosis and distinguishing between LTBI and ATB, and has high specificity and sensitivity. The lectin combination provided by the present application in combination with detection of specific antibodies of mycobacterium tuberculosis antigens can further improve the diagnostic effect. The marker and its detection products provided by the present application have good application potential in ATB diagnosis and distinguishing between LTBI and ATB, and are expected to overcome the limitations of existing diagnostic techniques, improve the diagnostic accuracy and sensitivity of tuberculosis, and provide new ideas and technical means for early detection, precise treatment and effective prevention and control of tuberculosis.

The present invention provides a method of detecting antibodies in a sample from a subject, wherein the antibodies bind to epitopes of Mycobacterium tuberculosis antigens, comprising contacting the sample with two or more isolated polypeptides or antigenic fragments or variants thereof, wherein the polypeptides comprise polypeptides selected from the group consisting of SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13, and 15; and detecting formation of antibody-peptide complexes comprising said isolated polypeptides or antigenic fragments or variants thereof, wherein formation of said complexes is indicative of the presence of the antibodies to epitopes of Mycobacterium tuberculosis antigens in said sample.

This invention relates to the field of in vitro diagnostic technology and provides a highly specific Mycobacterium tuberculosis antigen detection kit and detection method. The kit includes: a thermosensitive antigen aggregate composed of two or more of ESAT-6, CFP-10, and MPT-64 coupled with PNIPAM-b-PLL block copolymer, which spontaneously forms nanoscale antigen clusters at 37°C; dual-mechanism anti-fouling magnetic beads, which are Fe3O4@SiO2@PEG-polysulfobetaine three-layer core-shell structures, with a reference fluorescent dye embedded in the SiO₂ layer via covalent bonding, and the surface covalently coupled to the thermosensitive antigen aggregate via carboxyl groups; an AIE fluorescent probe, composed of AIE fluorescent microspheres coupled with an anti-tuberculosis antigen detection antibody; and a sample diluent. This invention achieves local enrichment of antigen through temperature-controlled aggregation, minimizes background through dual-mechanism anti-fouling magnetic beads (PEG steric hindrance + zwitterionic super hydration), and amplifies the signal and performs internal reference calibration through AIE ratio fluorescence. The three components work synergistically to achieve high sensitivity, low background, strong anti-interference ability, and easy operation for tuberculosis antigen detection.