1 results about "Mycobacterial antigen" patented technology

This invention relates to the field of in vitro diagnostic technology and provides a highly specific Mycobacterium tuberculosis antigen detection kit and detection method. The kit includes: a thermosensitive antigen aggregate composed of two or more of ESAT-6, CFP-10, and MPT-64 coupled with PNIPAM-b-PLL block copolymer, which spontaneously forms nanoscale antigen clusters at 37°C; dual-mechanism anti-fouling magnetic beads, which are Fe3O4@SiO2@PEG-polysulfobetaine three-layer core-shell structures, with a reference fluorescent dye embedded in the SiO₂ layer via covalent bonding, and the surface covalently coupled to the thermosensitive antigen aggregate via carboxyl groups; an AIE fluorescent probe, composed of AIE fluorescent microspheres coupled with an anti-tuberculosis antigen detection antibody; and a sample diluent. This invention achieves local enrichment of antigen through temperature-controlled aggregation, minimizes background through dual-mechanism anti-fouling magnetic beads (PEG steric hindrance + zwitterionic super hydration), and amplifies the signal and performs internal reference calibration through AIE ratio fluorescence. The three components work synergistically to achieve high sensitivity, low background, strong anti-interference ability, and easy operation for tuberculosis antigen detection.