Applications of Mycobacterium tuberculosis antigen protein Rv0446c and T-cell epitope peptide thereof

Abstract

The present invention relates to applications of Mycobacterium tuberculosis antigen protein Rv0446c and a T-cell epitope peptide thereof in preparation of tuberculosis detection reagents, vaccines and medicines, wherein the amino acid sequences of the antigen protein Rv0446c and the T-cell epitope peptide thereof are respectively represented by SEQ ID NO:1-5. According to the present invention, the Mycobacterium tuberculosis antigen protein Rv0446c and the T-cell epitope peptide thereof are used as the stimulants for the specific T cell and B cell immune response caused by Mycobacterium tuberculosis infection, and the false positive caused by the impure antigen can be reduced compared with the use of the complete antigen in the prior art; and the detection reagents prepared from the antigen protein Rv0446c and the T-cell epitope peptide thereof can be widely used for assisted diagnosis of tuberculosis, epidemiological surveillance and other related fields, and the tuberculosis vaccines and the anti-tuberculosis drugs prepared from the antigen protein Rv0446c and the T-cell epitope peptide thereof can be used for prevention and treatment of tuberculosis.

Description

technical field [0001] The invention relates to the fields of molecular biology and immunology, in particular to the application of mycobacterium tuberculosis antigen protein Rv0446c and its T cell epitope peptide in the preparation of tuberculosis detection reagents, vaccines and medicines. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Survey results show that one-third of the world's population is latently infected, and 5% to 10% may develop active tuberculosis in future life. Since the World Health Organization declared tuberculosis a global crisis in 1993, the morbidity and mortality of tuberculosis have remained high. According to the WHO report, there are about 8 million new tuberculosis patients every year, and about 2 to 3 million people die of tuberculosis every year. my country ranks second among the 22 countries with a high burden of tuberculosis. The results of the fifth national epidemiological s...

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Problems solved by technology

However, due to the high requirements for the bacterial content in the sample, the sensitivity of this method is not high, resulting in a large number of smear-negative patients being undetected and turning positive, and smear-negative patients are contagious, which cannot be ignored. This method has no species specificity and poor sensitivity , affected by sputum specimen and disease condition

Sputum culture is the gold standard for the diagnosis of tuberculosis, but the culture time is too long. Now there are rapid culture systems such as the BACTECMGIT960 system that can isolate and culture Mycobacterium tuberculosis within 2 weeks. Inhibitors are expensive and cannot be widely used in developing countries, and rapid cultures have significantly higher contamination rates compared to modified L-J cultures, leading to false positive results

The commonly used detection method for population screening is the skin tuberculin test, which uses pure protein derivatives of Mycobacterium tuberculosis (PPD), because PPD contains many mycobacterial species (pathogenic mycobacteria, environmental mycobacteria bacillus and BCG), so the specificity of PPD diagnosis of tuberculosis is poor, and it cannot effectively distinguish between Mycobacterium tuberculosis infection and BCG vaccination. Tuberculosis imaging examinations include routine X-ray examination, CT examination, MRI examination, Ultrasound examinations are expensive, cause certain harm to the body, have low specificity, and are not suitable for routine examination and diagnosis

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