The application of Mycobacterium tuberculosis antigenic protein rv0585c and its T-cell epitope peptide

Abstract

The invention relates to mycobacterium tuberculosis antigen protein Rv0585c and application of a T cell epitope peptide thereof in preparing tuberculosis detection reagents, vaccines and medicines. The epitope peptide is selected from P24, P26 and P31 which have amino acid sequences shown as SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 respectively. A mycobacterium tuberculosis protein antigen Rv0585c and the T cell epitope peptide thereof serve as irritants and are used for specific T cell and B cell immunoreaction caused by mycobacterium tuberculosis infection; compared with an existing adopted complete antigen, the false positive result caused by antigen impurity can be reduced. The detection reagents prepared from the protein antigen Rv0585c and the epitope peptide of the protein antigen Rv0585c can be widely used for auxiliary diagnosis, epidemiological monitoring and other related fields of tuberculosis. Tuberculosis vaccines and antituberculosis medicines prepared from the protein antigen Rv0585c and the epitope peptide of the protein antigen Rv0585c can be used for preventing and treating tuberculosis.

Description

technical field [0001] The invention relates to the fields of molecular biology and immunology, in particular to the application of mycobacterium tuberculosis antigen protein Rv0585c and its T cell epitope peptide in the preparation of tuberculosis detection reagents, vaccines and medicines. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Survey results show that one-third of the world's population is latently infected, and 5% to 10% may develop active tuberculosis in future life. Since the World Health Organization declared tuberculosis a global crisis in 1993, the morbidity and mortality of tuberculosis have remained high. According to the WHO report, there are about 8 million new tuberculosis patients every year, and about 2 to 3 million people die of tuberculosis every year. my country ranks second among the 22 countries with a high burden of tuberculosis. The results of the fifth national epidemiological s...

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Problems solved by technology

However, due to the high requirements for the bacterial content in the sample, the sensitivity of this method is not high, resulting in a large number of smear-negative patients being undetected and turning positive, and smear-negative patients are contagious, which cannot be ignored. This method has no species specificity and poor sensitivity , affected by sputum specimen and disease condition

Sputum culture is the gold standard for the diagnosis of tuberculosis, but the culture time is too long. Now there are rapid culture systems such as the BACTECMGIT960 system that can isolate and culture Mycobacterium tuberculosis within 2 weeks. Inhibitors are expensive and cannot be widely used in developing countries, and rapid cultures have significantly higher contamination rates compared to modified L-J cultures, leading to false positive results

The commonly used detection method for population screening is the skin tuberculin test, which uses pure protein derivatives of Mycobacterium tuberculosis (PPD), because PPD contains many mycobacterial species (pathogenic mycobacteria, environmental mycobacteria bacillus and BCG), so the specificity of PPD diagnosis of tuberculosis is poor, and it cannot effectively distinguish between Mycobacterium tuberculosis infection and BCG vaccination. Tuberculosis imaging examinations include routine X-ray examination, CT examination, MRI examination, Ultrasound examinations are expensive, cause certain harm to the body, have low specificity, and are not suitable for routine examination and diagnosis

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