The application of Mycobacterium tuberculosis antigenic protein rv2201 and its T-cell epitope peptide

Abstract

The invention relates to application of a mycobacterium tuberculosis antigen protein Rv2201 and a T cell epitope peptide thereof to preparation of a tuberculosis detection reagent, vaccine and medicament. Amino acid sequences of the mycobacterium tuberculosis antigen protein Rv2201 and the T cell epitope peptide thereof are shown as SEQ ID NO:1-3. The mycobacterium tuberculosis antigen protein Rv2201 and the T cell epitope peptide thereof are adopted for immunoreaction of specific T cells and B cells caused by mycobacterium tuberculosis infection as irritants, and compared with conventional adoption of a complete antigen, adoption of the mycobacterium tuberculosis antigen protein Rv2201 and the T cell epitope peptide thereof has the advantage that a false positive result caused by impurity of the antigen can be reduced. The detection reagent prepared from the antigen protein Rv2201 and the epitope peptide thereof can be widely applied to the related fields of auxiliary diagnosis of tuberculosis, epidemiological monitoring and the like, and the tuberculosis vaccine and anti-tuberculosis medicament prepared from the antigen protein Rv2201 and the epitope peptide thereof can be used for prevention and treatment of tuberculosis.

Description

technical field [0001] The invention relates to the fields of molecular biology and immunology, in particular to the application of mycobacterium tuberculosis antigen protein Rv2201 and its T cell epitope peptide in the preparation of tuberculosis detection reagents, vaccines and medicines. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Survey results show that one-third of the world's population is latently infected, and 5% to 10% may develop active tuberculosis in future life. Since the World Health Organization declared tuberculosis a global crisis in 1993, the morbidity and mortality of tuberculosis have remained high. According to the WHO report, there are about 8 million new tuberculosis patients every year, and about 2 to 3 million people die of tuberculosis every year. my country ranks second among the 22 countries with a high burden of tuberculosis. The results of the fifth national epidemiological sa...

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Problems solved by technology

However, due to the high requirements for the bacterial content in the sample, the sensitivity of this method is not high, resulting in a large number of smear-negative patients being undetected and turning positive, and smear-negative patients are contagious, which cannot be ignored. This method has no species specificity and poor sensitivity , affected by sputum specimen and disease condition

Sputum culture is the gold standard for the diagnosis of tuberculosis, but the culture time is too long. Now there are rapid culture systems such as the BACTEC MGIT960 system that can isolate and culture Mycobacterium tuberculosis within 2 weeks. Bacterial inhibitors are expensive and cannot be widely promoted in developing countries, and the contamination rate of rapid culture is significantly higher than that of improved L-J culture, resulting in false positive results

The commonly used detection method for population screening is the skin tuberculin test, which uses pure protein derivatives of Mycobacterium tuberculosis (PPD), because PPD contains many mycobacterial species (pathogenic mycobacteria, environmental mycobacteria bacillus and BCG), so the specificity of PPD diagnosis of tuberculosis is poor, and it cannot effectively distinguish between Mycobacterium tuberculosis infection and BCG vaccination. Tuberculosis imaging examinations include routine X-ray examination, CT examination, MRI examination, Ultrasound examinations are expensive, cause certain harm to the body, have low specificity, and are not suitable for routine examination and diagnosis

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