Suspension array for detecting serotype enterobacter aerogenes typing and application

Abstract

The invention provides a specific oligonucleotide sequence for detecting serotypes 1-8 of enterobacter aerogenes. The oligonucleotide contains: (1), a DNA fragment selected from wzy genes of the enterobacter aerogenes of types 1, 2, 3, 5, 6, 7 and 8; (2), a DNA fragment selected from orf8 genes of the enterobacter aerogenes of type 4. A suspension array detection system and method for the serotypes 1-8 of the enterobacter aerogenes are established through combination with a Bio-Plex 200 suspension array system of the Bio-Rad company, the blank of serological typing technology of the enterobacter aerogenes is filled up, and the invention has great significance in clinical detection and epidemiological monitoring of the important pathogenic bacterium.

Description

technical field [0001] The invention belongs to the technical field of bacterial detection methods, and relates to a suspension chip used for typing Enterobacter aerogenes common serotypes in my country and its application. Background technique [0002] Serological typing has been widely used since the 1930s and has a history of more than 80 years. It can further classify bacteria within species / genus and is an important method of bacterial identification. At present, serological identification methods are used The most common method for identification and traceability of pathogenic bacteria. For example: our country targets Shigella (GB / T 4789.5-2012), diarrhea-causing E. coli (GB / T 4789.6-2003), E. Bacteria (GB / T4789.7-2013), Salmonella (GB / T 4789.4-2010) and Yersinia enterocolitica (GB / T 4789.8-2008) and other pathogenic bacteria detection standards have adopted serological identification Methods. Serological identification is mainly based on the immunogenicity of bact...

AI Technical Summary

Problems solved by technology

[0004] 1) The work of establishing a serological typing system is cumbersome and takes a long time. For example, it took more than 30 years (about 1944-1977) to establish a relatively comprehensive E. coli serological typing system; therefore, the serological classification of some important pathogenic bacteria No type system has yet been established, which has brought great difficulties to the identification, monitoring and traceability of these pathogenic bacteria;

[0005] 2) The production and quality control of antiserum are relatively difficult. In order to avoid cross-reaction, a large number of adsorption reactions are required in the production of antiserum;

[0006] 3) Many antisera are produced and stored by only a few units in the world, and the antisera used for identification are seriously incomplete. The main companies that produce bacterial antisera in the world are Difco in the United States, Denka Seiken in Japan, and S&A in Thailand. Provide less than 20% of the species actually needed; 4) The experimental process of serological identification takes a long time. Taking Salmonella as an example, it takes 2-6 days from obtaining pure culture to completing serotype identification

Moreover, due to the strong drug resistance of the bacteria, it brings difficulties to clinical anti-infection treatment.

Unlike some other important pathogenic bacteria, such as Escherichia coli, Salmonella, Vibrio parahaemolyticus, etc., there is no report on the serological typing system of Enterobacter aerogenes

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