On-site rapid high-sensitivity detection kit and detection method of prawn yellow head virus

Abstract

The invention relates to an on-site rapid high-sensitivity detection kit and a detection method of prawn yellow head virus. The on-site rapid high-sensitivity detection kit comprises a sampling tube, a rinsing tube filled with distilled water, a nucleic acid denaturation tube filled with TE buffer liquid, an amplification detection tube filled with amplification reaction liquid and nucleic acid dye, a negative control tube, a positive control tube, an FTA membrane, quick drying liquid, and the like. Compared with an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method, the detection method has higher specificity, sensitivity and convenience, and has the advantages of low cost, convenient use, more accurate and quicker detection, high sensitivity, and high safety to human and environment, and can replace the traditional related detection methods, such as an electron-microscopic observation method, an RT-PCR detection method, and the like. The on-site rapid high-sensitivity detection kit and the detection method can be used in both an indoor laboratory and a production site in the field, have great significance for strengthening the prawn yellow head virus epidemiological monitoring, prawn culturing water monitoring and disease control and prevention, and have high popularization and application value.

Description

technical field [0001] The invention relates to a marine biological pathogen detection technology, in particular to an on-site rapid and high-sensitivity detection kit for prawn yellow head virus (Taura syndrome virus, YHV for short) and a detection method thereof. Background technique [0002] Shrimp yellowhead virus is a single-stranded RNA virus with a positive-sense genome. It belongs to the family Roniviridae and the genus Okavirus. The virus mainly infects Penaeus monodon and Marsupenaeus japonicus. , Penaeus merguiensis (Fenneropenaeus merguiensis), Penaeus merguiensis (Litopenaeus setiferus), Penaeus ensis (Metapenaeus ensis) and Antarctic krill (Euphasia superba), etc. Among them, Penaeus monodon is the most sensitive, and Penaeus monodon died after being infected with the virus The rate is almost 100%, and the virus has caused huge losses in shrimp farming in countries such as Australia, India, Vietnam, and Thailand. In recent years, with the expansion of the scal...

AI Technical Summary

Problems solved by technology

The histopathological observation method cannot directly detect the virus, but can only detect the histopathological signs of the disease, and it is also limited to the laboratory; although the electron microscope technology can directly observe the presence of virus particles, its operation is complicated , time-consuming and low accuracy; although the RT-PCR detection method of YHV overcomes the shortcomings of the previous methods, it can realize relatively fast and accurate detection of YHV under laboratory conditions, but due to the conventional RT-PCR Detection requires expensive PCR machines, gel electrophoresis and imaging systems, making it difficult for RT-PCR detection methods to be used for rapid on-site detection, which greatly limits the popularization and application of RT-PCR detection methods in production