Field fast high-sensitivity detection kit and detection method for prawn hepatopancreatic parvovirus

Abstract

The invention relates to a field fast high-sensitivity detection kit and a detection method for prawn hepatopancreatic parvovirus. The detection kit comprises a sampling pipe, a rinsing pipe filled with distilled water, a nucleic acid denaturation pipe filled with a TE buffer solution, an amplification detection pipe filled with amplification reactant liquid and nucleic acid dyes, a negative control pipe, a positive control pipe, an FTA diaphragm, quick drying liquid thereof and the like. The detection method has higher specificity, sensitivity and convenience than that of the PCR detection method, has the advantages of low cost, convenient operation, more accurate and faster detection and extremely high sensitivity, is safe to both human beings and the environment, and can replace the existing relevant detection methods such as the pathological section method, the electron microscope method and the PCR detection method. The detection kit and the detection method can be used in the indoor laboratory or the outdoor production field, are of great significance for enhancing the epidemiological monitoring and the disease control on prawn hepatopancreatic parvovirus, and have higher popularization and application values.

Description

technical field [0001] The invention relates to a marine biological pathogen detection technology, in particular to an on-site rapid and highly sensitive detection kit for shrimp hepatopancreatic parvovirus (Hepatopancreatic Parvovirus, referred to as HPV) and a detection method thereof. Background technique [0002] Shrimp hepatopancreatic parvovirus, also known as DNA parvovirus (Parvovirus), is a single-stranded DNA virus belonging to the family Parvoviridae and the genus Parvovirus. HPV can infect Chinese prawns (Fenneropenaeus chinensis), vannamei (Litopenaeus vannamei), Indian prawns (Fenneropenaeus indieus), monodon prawns (Penaeus monodon), Japanese sac prawns (Marsupenaeus japonicus), Mexican prawns (Fenneropenaeus merguiensis), Tiger prawn (browntigerprawn, Penaeus esculentus), long-haired prawn (Fenneropenaeus penicillatus), short groove prawn (Penaeus semisulcatus) and blue prawn (blue shrimp, Litopenaeusstylirostris) and other important economic prawns and wild ...

AI Technical Summary

Problems solved by technology

The pathological section method cannot directly detect the virus, but can only detect the histopathological signs of the disease, and it is also limited to the laboratory; although the electron microscope technology can directly observe the existence of virus particles, its operation is complicated and costly. The time is long and the accuracy is low; although the PCR detection method of HPV overcomes the shortcomings of the previous methods, it can realize relatively fast and accurate detection of HPV under laboratory conditions, but because conventional PCR detection requires expensive PCR instruments , gel electrophoresis and imaging systems, making it difficult for the PCR detection method to be used for on-site rapid detection, which greatly limits the promotion and application of the PCR detection method in on-site production