A composition containing superoxide dismutase (SOD) and its preparation method
Through the combination of yeast fermentation product filtrate, garlic bulb extract, superoxide dismutase and angelica extract, fibroblasts are activated and collagen production is promoted, and the problem of poor wrinkle reduction in existing skin care products is solved, and the problem of existing skin care products is not effective. Quickly fade wrinkles and anti-wrinkle firming effects.
Patent Information
- Application Number
- CN202411346060.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-26
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2044-09-26
AI Technical Summary
Existing anti-aging skin care products have relatively average effects in reducing wrinkles, which is difficult to meet consumers' needs to quickly reduce wrinkles.
Using a composition containing yeast fermentation product filtrate, garlic bulb extract, superoxide dismutase (SOD) and angelica extract, the various active ingredients in the yeast fermentation product filtrate cooperate with each other to activate fibroblasts and promote the Collagen production and secretion, quickly replenish skin collagen and achieve rapid fading of wrinkles.
This composition can quickly remove free radicals from the skin, promote the production and secretion of collagen, significantly reduce wrinkles, achieve the effect of quickly smoothing wrinkles, restore the skin to youthfulness, and have significant anti-wrinkle firming effects.
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Abstract
Description
Technical Field
[0001] The invention relates to the field of skin care products, in particular to a composition containing superoxide dismutase (SOD) and a preparation method thereof. Background Art
[0002] Wrinkles refer to the small fine lines and wrinkles formed when the skin is affected by the external environment and free radicals are formed. Free radicals destroy collagen and active substances in normal cell membrane tissues and oxidize cells.
[0003] The appearance of wrinkles will seriously affect the appearance. With the development of technology, skin care products are no longer a high-priced luxury product, but have become daily consumer products for most consumers. By using skin care products to repair the skin, the skin can be improved and made healthier. Many skin care products have anti-aging effects by adding substances that have the effect of scavenging free radicals, and are deeply loved by consumers.
[0004] Although there are many anti-aging products on the market, the effect of these anti-aging products in reducing wrinkles is relatively general, and the wrinkle reduction period is relatively long, which cannot meet consumers' demand for quick wrinkle reduction. Therefore, there is still room for improvement. Summary of the invention
[0005] In order to improve the anti-wrinkle and firming effect to achieve rapid wrinkle reduction, the present application provides a composition containing superoxide dismutase SOD and a preparation method thereof.
[0006] In the first aspect, the present application provides a composition containing superoxide dismutase (SOD), which adopts the following technical solution:
[0007] A composition containing superoxide dismutase (SOD), comprising the following components in parts by weight:
[0008] 28-32 parts of yeast fermentation product filtrate;
[0009] Garlic bulb extract 0.8-1.2 parts;
[0010] Superoxide dismutase (SOD) 0.8-1.2 parts;
[0011] 0.4-0.6 parts of angelica extract;
[0012] 65-70 parts of water;
[0013] The preparation method of the yeast fermentation product filtrate is as follows:
[0014] Step 1), evenly mix broom, hops, hibiscus bark and perilla leaves to obtain a plant mixture;
[0015] Step 2), mixing the plant mixture with water to obtain a plant mixture solution;
[0016] Step 3), inoculating Candida bombus and Cryptococcus laurentii into the plant mixture, fermenting for 7-9 days, filtering, sterilizing, and obtaining a yeast fermentation product filtrate.
[0017] By adopting the above technical scheme, by adding garlic bulb extract, superoxide dismutase (SOD) and angelica extract, a good effect of scavenging free radicals can be achieved. The composition containing superoxide dismutase SOD is applied to the skin, which can quickly scavenges free radicals in the skin, and the effect of scavenging free radicals is relatively long-lasting, making the skin less susceptible to free radical attacks, reducing skin damage and preventing new wrinkles from forming.
[0018] By adding yeast fermentation product filtrate, since the yeast fermentation product filtrate is compounded by broom, hops, hibiscus bark, and perilla leaves and then fermented by bumblebee Candida and Laurentian Cryptococcus, the yeast fermentation product filtrate contains a variety of special active ingredients. After the multiple active ingredients cooperate with each other, they can promote the production of collagen, so that the collagen lost in the skin can be quickly replenished, and because the activity of fibroblasts in the skin is activated, the secreted collagen will not be rejected. Compared with directly supplementing collagen from the outside, the collagen secreted by fibroblasts is less likely to be lost, so that the skin can quickly accumulate collagen, thereby re-supporting the skin, so that wrinkles on the skin surface can be quickly faded, thereby achieving the effect of smoothing wrinkles, restoring the skin to youth, and making the anti-wrinkle and firming effect better.
[0019] Preferably, in step 1), the mass ratio of broom, hops, hibiscus bark and perilla leaf is 16-18:8-10:3-5:7-9.
[0020] By adopting the above technical scheme and specifically selecting the mass ratio of broom, hops, hibiscus bark and perilla leaves, various special active ingredients in the filtrate of yeast fermentation products are combined in special ratios, which can better activate fibroblasts, secrete collagen faster, and smooth wrinkles more efficiently.
[0021] Preferably, in step 2), the mass ratio of the plant mixture to water is 34-42:170-210.
[0022] By adopting the above technical scheme and specifically selecting the mass ratio of the mixture to water, the concentration of active ingredients in the yeast fermentation product filtrate is just right, which can better activate the activity of fibroblasts to accelerate the secretion of collagen, while better controlling costs and having high economic value.
[0023] Preferably, in step 3), the mass ratio of Candida bombus to Cryptococcus laurentii is 0.07-0.09:0.04-0.06.
[0024] By adopting the above technical scheme and specifically selecting the mass ratio of Candida bombus and Cryptococcus laurentii, the fermentation effect is better, the various special active ingredients in the fermentation product are better matched in special ratios, and the effect of activating fibroblast activity to accelerate the secretion of collagen is more significant.
[0025] Preferably, in step 3), the mass ratio of the plant mixture, Candida bombus, and Cryptococcus laurentii is 204-252: 0.07-0.09: 0.04-0.06.
[0026] By adopting the above technical scheme and specifically selecting the mass ratio of the plant mixture, Candida albicans bumblebee and Cryptococcus laurentii, the fermentation effect is better, while the cost is effectively controlled, and the product has a higher economic value.
[0027] Preferably, in the step 1), broom, hops, hibiscus bark and perilla leaves are mixed and crushed into powder with a particle size of 1-2 mm.
[0028] By adopting the above technical scheme, the active ingredients in broom, hops, hibiscus bark and perilla leaves are easier to extract by crushing them into powder, and the quality of the obtained yeast fermentation product filtrate is better, and the effect of repairing the skin is better.
[0029] Preferably, in the step 2), the plant mixture and water are uniformly mixed, heated to boiling, condensed and refluxed, extracted for 2-3 hours, and cooled to room temperature to obtain a plant mixture.
[0030] By adopting the above technical scheme, the active ingredients in broom, hops, hibiscus bark and perilla leaves can be better extracted into water through heating extraction, and the active ingredients can be better fermented by bumblebee Candida albicans and Cryptococcus laurentii. The quality of the obtained yeast fermentation product filtrate is better and the skin care effect is better.
[0031] Preferably, in step 3), after Candida bombus and Cryptococcus laurentii are inoculated into the plant mixture, the mixture is kept at a constant temperature of 28-32° C. and fermented for 7-9 days.
[0032] By adopting the above technical scheme and through constant temperature fermentation, the activity of Candida albicans and Cryptococcus laurentii can be higher, the fermentation efficiency can be improved, the process time can be shortened, and the economic value can be higher.
[0033] Preferably, the garlic bulb extract and angelica extract are both water-soluble extracts.
[0034] By adopting the above technical scheme and specifically selecting water-soluble extracts, the skin can be more easily absorbed, and the skin care effect of the superoxide dismutase SOD composition is more significant, and the anti-wrinkle and firming effect is more significant.
[0035] In a second aspect, the present application provides a method for preparing a composition containing superoxide dismutase SOD, using the following technical solution:
[0036] A method for preparing the above-mentioned superoxide dismutase (SOD) composition comprises the following steps:
[0037] Step 01), uniformly mix garlic bulb extract, superoxide dismutase (SOD), angelica extract and water to obtain a premix;
[0038] Step 02), add the yeast fermentation product filtrate to the premix, mix evenly, and obtain a composition containing superoxide dismutase SOD.
[0039] By adopting the above technical scheme, the obtained composition containing superoxide dismutase SOD can quickly remove free radicals in the skin, can continuously protect the skin, reduce the erosion of the skin by free radicals, and can stimulate the activity of fibroblasts, accelerate the secretion of collagen, and make the collagen in the skin accumulate rapidly, thereby achieving the effect of quickly fading and smoothing wrinkles.
[0040] In summary, this application has the following beneficial effects:
[0041] 1. Since the present application adds garlic bulb extract, superoxide dismutase (SOD) and angelica extract, it can play a good effect of scavenging free radicals. The composition containing superoxide dismutase SOD is applied to the skin to quickly remove free radicals in the skin. Then, by adding yeast fermentation product filtrate, since the yeast fermentation product filtrate is compounded with broom, hops, hibiscus bark and perilla leaves and then fermented by bumblebee Candida and Laurentiana, the yeast fermentation product filtrate contains a variety of special active ingredients. After the multiple active ingredients cooperate with each other, they can promote the production of collagen, so that the collagen lost in the skin can be quickly replenished, so that the skin can quickly accumulate collagen, thereby re-supporting the skin, and wrinkles on the skin surface can be quickly faded, thereby achieving the effect of smoothing wrinkles, making the skin regain youthfulness, and making the anti-wrinkle and firming effect better.
[0042] 2. In the present application, it is preferred to specifically select the mass ratio of broom, hops, hibiscus bark and perilla leaf so that various special active ingredients in the filtrate of yeast fermentation product are combined in a special ratio, which can better activate fibroblasts, secrete collagen faster, and smooth wrinkles more efficiently. DETAILED DESCRIPTION
[0043] The present application is further described in detail below with reference to the embodiments. Example 1
[0044] A composition containing superoxide dismutase (SOD), comprising the following components:
[0045] Saccharomyces fermentation product filtrate, garlic bulb extract, superoxide dismutase (SOD), angelica extract, water.
[0046] The yeast fermentation product filtrate is homemade and the preparation method is as follows:
[0047] Step 1), 16 kg of broom, 8 kg of hops, 3 kg of hibiscus bark, and 7 kg of perilla leaves are mixed evenly, crushed into powder with a particle size of 1-2 mm, and then put into a stirring kettle at a speed of 60 r / min and stirred for 5 minutes to obtain a plant mixture.
[0048] Step 2), add 170kg of water to the plant mixture, rotate at 120r / min, stir for 5min, mix evenly, heat to boiling, condense and reflux, extract for 2h, cool to room temperature, and obtain a plant mixture.
[0049] Step 3), inoculate 0.07kg of Candida bombus and 0.04kg of Cryptococcus laurentii into the plant mixture, keep the temperature at 28°C, ferment for 7 days, filter with 80-mesh filter cloth, remove the filter residue, take the filtrate, filter the filtrate through a 0.22 μm filter membrane for sterilization, take the filtrate, and obtain the yeast fermentation product filtrate.
[0050] Broom, hops, hibiscus bark and perilla leaves are all commercially available and are sun-dried products.
[0051] Bombus candida was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., with the strain number: ATCC 22214.
[0052] Cryptococcus laurensis was purchased from Beijing Biobio Biotechnology Co., Ltd. with the strain number: ATCC 66036.
[0053] Garlic bulb extract was purchased from Jiahao Biotechnology (Guangzhou) Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.
[0054] Superoxide dismutase (SOD) was purchased from Guangzhou Shizhen Herbal Medicine Research Co., Ltd.
[0055] Angelica extract was purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.
[0056] The preparation method of the composition containing superoxide dismutase SOD comprises the following steps:
[0057] Step 01), 0.8 kg of garlic bulb extract, 0.8 kg of superoxide dismutase (SOD), 0.4 kg of angelica extract, and 65 kg of water are put into a stirring tank, the speed is 120 r / min, stirring for 15 minutes, mixing evenly, and obtaining a premix.
[0058] Step 02), add 28 kg of yeast fermentation product filtrate to the premix, rotate at 120 r / min, stir for 15 min, mix evenly, and obtain a composition containing superoxide dismutase SOD. Example 2
[0059] A composition containing superoxide dismutase (SOD), comprising the following components:
[0060] Saccharomyces fermentation product filtrate, garlic bulb extract, superoxide dismutase (SOD), angelica extract, water.
[0061] The yeast fermentation product filtrate is homemade and the preparation method is as follows:
[0062] Step 1), 17 kg of broom, 9 kg of hops, 4 kg of hibiscus bark, and 8 kg of perilla leaves are mixed evenly, crushed into powder with a particle size of 1-2 mm, and then put into a stirring kettle, the speed is 60 r / min, and stirred for 5 minutes to obtain a plant mixture.
[0063] Step 2), add 190 kg of water to the plant mixture, rotate at 120 r / min, stir for 5 min, mix evenly, heat to boiling, condense and reflux, extract for 2.5 h, cool to room temperature, and obtain a plant mixture.
[0064] Step 3), inoculate 0.08kg of Candida bombus and 0.05kg of Cryptococcus laurentii into the plant mixture, keep the temperature at 30°C, ferment for 8 days, filter with 80-mesh filter cloth, remove the filter residue, take the filtrate, filter the filtrate through a 0.22μm filter membrane for sterilization, take the filtrate, and obtain the yeast fermentation product filtrate.
[0065] Broom, hops, hibiscus bark and perilla leaves are all commercially available and are sun-dried products.
[0066] Bombus candida was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., with the strain number: ATCC 22214.
[0067] Cryptococcus laurensis was purchased from Beijing Biobio Biotechnology Co., Ltd. with the strain number: ATCC 66036.
[0068] Garlic bulb extract was purchased from Jiahao Biotechnology (Guangzhou) Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.
[0069] Superoxide dismutase (SOD) was purchased from Guangzhou Shizhen Herbal Medicine Research Co., Ltd.
[0070] Angelica extract was purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.
[0071] The preparation method of the composition containing superoxide dismutase SOD comprises the following steps:
[0072] Step 01), put 1kg of garlic bulb extract, 1kg of superoxide dismutase (SOD), 0.5kg of angelica extract, and 67.5kg of water into a stirring tank, rotate at 120r / min, stir for 15min, mix evenly, and obtain a premix.
[0073] Step 02), add 30kg of yeast fermentation product filtrate to the premix, rotate at 120r / min, stir for 15min, mix evenly, and obtain a composition containing superoxide dismutase SOD. Example 3
[0074] A composition containing superoxide dismutase (SOD), comprising the following components:
[0075] Saccharomyces fermentation product filtrate, garlic bulb extract, superoxide dismutase (SOD), angelica extract, water.
[0076] The yeast fermentation product filtrate is homemade and the preparation method is as follows:
[0077] Step 1), 18 kg of broom, 10 kg of hops, 5 kg of hibiscus bark, and 9 kg of perilla leaves are mixed evenly, crushed into powder with a particle size of 1-2 mm, and then put into a stirring kettle, the speed is 60 r / min, and stirred for 5 minutes to obtain a plant mixture.
[0078] Step 2), add 210 kg of water to the plant mixture, rotate at 120 r / min, stir for 5 min, mix evenly, heat to boiling, condense and reflux, extract for 3 h, cool to room temperature, and obtain a plant mixture.
[0079] Step 3), inoculate 0.09 kg of Candida bombus and 0.06 kg of Cryptococcus laurentii into the plant mixture, keep the temperature at 32°C, ferment for 9 days, filter with 80-mesh filter cloth, remove the filter residue, take the filtrate, filter the filtrate through a 0.22 μm filter membrane for sterilization, take the filtrate, and obtain the yeast fermentation product filtrate.
[0080] Broom, hops, hibiscus bark and perilla leaves are all commercially available and are sun-dried products.
[0081] Bombus candida was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., with the strain number: ATCC 22214.
[0082] Cryptococcus laurensis was purchased from Beijing Biobio Biotechnology Co., Ltd. with the strain number: ATCC 66036.
[0083] Garlic bulb extract was purchased from Jiahao Biotechnology (Guangzhou) Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.
[0084] Superoxide dismutase (SOD) was purchased from Guangzhou Shizhen Herbal Medicine Research Co., Ltd.
[0085] Angelica extract was purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd., a water-soluble extract with an extraction ratio of 10:1.
[0086] The preparation method of the composition containing superoxide dismutase SOD comprises the following steps:
[0087] Step 01), 1.2 kg of garlic bulb extract, 1.2 kg of superoxide dismutase (SOD), 0.6 kg of angelica extract, and 70 kg of water are put into a stirring tank, the speed is 120 r / min, stirring for 15 minutes, mixing evenly, and obtaining a premix.
[0088] Step 02), add 32kg of yeast fermentation product filtrate to the premix, rotate at 120r / min, stir for 15min, mix evenly, and obtain a composition containing superoxide dismutase SOD.
[0089] Comparative Example 1
[0090] A composition containing superoxide dismutase (SOD), compared with Example 2, the only difference is:
[0091] In the method for preparing the filtrate of yeast fermentation product, an equal amount of Glycyrrhiza glabra is used to replace Broom.
[0092] Glycyrrhiza glabra is commercially available and is a sun-dried product.
[0093] Comparative Example 2
[0094] A composition containing superoxide dismutase (SOD), compared with Example 2, the only difference is:
[0095] In the method for preparing yeast fermentation product filtrate, an equal amount of gardenia is used to replace hops.
[0096] Gardenia flowers are commercially available and are sun-dried.
[0097] Comparative Example 3
[0098] A composition containing superoxide dismutase (SOD), compared with Example 2, the only difference is:
[0099] In the method for preparing the yeast fermentation product filtrate, an equal amount of Salvia miltiorrhiza is used to replace the Radix Sophorae Fructus.
[0100] Salvia miltiorrhiza is commercially available and is a sun-dried product.
[0101] Comparative Example 4
[0102] A composition containing superoxide dismutase (SOD), compared with Example 2, the only difference is:
[0103] In the method for preparing the yeast fermentation product filtrate, an equal amount of ginkgo leaves is used to replace perilla leaves.
[0104] Ginkgo biloba leaves are commercially available and are sun-dried.
[0105] Comparative Example 5
[0106] A composition containing superoxide dismutase (SOD), compared with Example 2, the only difference is:
[0107] In the preparation method of the yeast fermentation product filtrate, an equal amount of Glycyrrhiza glabra is used to replace Broom Flower; an equal amount of Gardenia jasminoides is used to replace Hops; an equal amount of Salvia miltiorrhiza is used to replace Hibiscus sabdariffa; and an equal amount of Ginkgo biloba leaf is used to replace Perilla frutescens leaf.
[0108] Glycyrrhiza glabra, Gardenia jasminoides, Salvia miltiorrhiza and Ginkgo biloba leaves are all commercially available and are sun-dried products.
[0109] Comparative Example 6
[0110] A composition containing superoxide dismutase (SOD), compared with Example 2, the only difference is:
[0111] In the method for preparing the yeast fermentation product filtrate, an equal amount of Candida glabrata is used to replace Candida bumblebee.
[0112] Candida glabrata was purchased from Beijing Biobio Biotechnology Co., Ltd. with the strain number: ATCC 2001.
[0113] Comparative Example 7
[0114] A composition containing superoxide dismutase (SOD), compared with Example 2, the only difference is:
[0115] In the method for preparing the yeast fermentation product filtrate, an equal amount of Issatchenia orientalis is used to replace Cryptococcus laurentii.
[0116] Issatchengensis yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., with the strain number: ATCC 6258.
[0117] Comparative Example 8
[0118] A composition containing superoxide dismutase (SOD), compared with Example 2, the only difference is:
[0119] In the preparation method of the yeast fermentation product filtrate, Candida glabrata is used to replace Candida bumblebee in equal amounts; Issatchenia orientalis is used to replace Cryptococcus laurentii in equal amounts.
[0120] Candida glabrata was purchased from Beijing Biobio Biotechnology Co., Ltd. with the strain number: ATCC 2001.
[0121] Issatchengensis yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., with the strain number: ATCC 6258.
[0122] Experiment 1
[0123] A number of volunteers were recruited and divided into groups to use the test samples of various embodiments and comparative examples.
[0124] When using the test sample, first wash your face with a disposable face towel and clean water, then apply 3 ml of the test sample evenly on the face, once in the morning and once in the evening, for 90 consecutive days. Do not use other skin care products during the experiment.
[0125] Before using the test sample, the volunteer's cheek was scraped 10 times with a cotton swab to scrape off the keratinocytes on the surface layer. The keratinocytes were placed in deionized water and stirred evenly to form a cell solution. Luminol reagent was used as a luminescent reagent, Fe2+ ions were used as a catalyst, and chemiluminescence was used. The luminescence intensity was detected by a fluorescence quantitative detection device and recorded as the initial luminescence intensity.
[0126] After using the test sample for 90 days, the volunteer's cheek was scraped 10 times with a cotton swab to scrape off the keratinocytes on the surface layer. The keratinocytes were placed in deionized water and stirred evenly to form a cell solution. Luminol reagent was used as a luminescent reagent, Fe2+ ions were used as a catalyst, and the chemiluminescence determination method was used. The luminescence intensity was detected by a fluorescence quantitative detection device and recorded as the luminescence intensity after repair.
[0127] The luminous intensity difference is obtained by subtracting the luminous intensity after repair from the initial luminous intensity.
[0128] Before using the test samples, the skin wrinkle status of the volunteers was measured using the skin fast three-dimensional imaging system PRIMOS to obtain the initial wrinkle measurement value.
[0129] After using the test samples for 90 days, the skin wrinkle status of the volunteers was measured using the skin fast three-dimensional imaging system PRIMOS to obtain the wrinkle measurement value after repair.
[0130] The difference in wrinkle measurements was obtained by subtracting the wrinkle measurements after repair from the initial wrinkle measurements.
[0131] Each group of experiments was conducted by 10 volunteers, and the experimental data of each group were averaged.
[0132] Sample to be tested: containing the original solution of superoxide dismutase SOD composition.
[0133] The specific test data of Experiment 1 are shown in Table 1.
[0134] Table 1
[0135]
[0136] According to the data comparison in Table 1, the difference in luminous intensity between each embodiment and each comparative example is similar, which shows that each embodiment and comparative example has a good effect of scavenging free radicals, but the difference in wrinkle measurement values of each embodiment is significantly greater than that of each comparative example, which shows that the use of the test samples of each embodiment can reduce wrinkles faster and achieve the effect of quickly smoothing wrinkles, proving that the yeast fermentation product filtrate obtained by mixing broom, hops, hibiscus bark, and perilla leaves and fermenting with bumblebee Candida and Cryptococcus laurentii can effectively promote the activity of fibroblasts in the skin, so that collagen can be quickly secreted inside the skin, thereby supporting the skin, making the skin smooth, and quickly fading and smoothing wrinkles.
[0137] In Comparative Examples 1-5, a single plant with the ability to scavenge free radicals was used to replace all of broom, hops, hibiscus bark, and perilla leaves. Although this resulted in better free radical scavenging effects on the test samples, its effects on promoting fibroblast synthesis and collagen secretion were weak, resulting in a slow wrinkle-reducing effect despite free radical scavenging effects.
[0138] In Comparative Examples 6-8, other fermentation strains were used to replace Candida bumblebee and Cryptococcus laurentii alone or in whole, resulting in changes in the fermentation products, destroying the special active ingredient formulation system in the examples, resulting in a decrease in the effect of promoting fibroblast synthesis and collagen secretion, and slowing down the efficiency of smoothing wrinkles.
[0139] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.
Claims
1. A composition containing superoxide dismutase (SOD), characterized in that: The composition includes the following components in parts by weight: 28-32 parts of yeast fermentation product filtrate; Garlic bulb extract 0.8-1.2 parts; Superoxide dismutase SOD 0.8-1.2 parts; 0.4-0.6 parts of angelica extract; 65-70 parts of water; The preparation method of the yeast fermentation product filtrate is as follows: Step 1), evenly mix broom, hops, hibiscus bark and perilla leaves to obtain a plant mixture; Step 2), mixing the plant mixture with water to obtain a plant mixture solution; Step 3), inoculating Candida bombus and Cryptococcus laurentii into the plant mixture, fermenting for 7-9 days, filtering, sterilizing, and obtaining a yeast fermentation product filtrate; In the step 1), the mass ratio of broom, hops, hibiscus bark and perilla leaf is 16-18:8-10:3-5:7-9; In the step 2), the mass ratio of the plant mixture to water is 34-42:170-210; In the step 3), the mass ratio of the plant mixture, Candida bombus, and Cryptococcus laurentii is 204-252: 0.07-0.09: 0.04-0.06; In the step 1), broom, hops, hibiscus bark and perilla leaves are mixed and crushed into powder with a particle size of 1-2 mm; In the step 2), the plant mixture and water are uniformly mixed, heated to boiling, condensed and refluxed, extracted for 2-3 hours, and cooled to room temperature to obtain a plant mixture; In the step 3), after inoculating Candida bombus and Cryptococcus laurentii into the plant mixture, the temperature is kept at 28-32° C. and fermented for 7-9 days; The garlic bulb extract and angelica extract are both water-soluble extracts.
2. A method for preparing a composition containing superoxide dismutase (SOD) according to claim 1, characterized in that: The following steps are involved: Step 01), uniformly mix garlic bulb extract, superoxide dismutase SOD, angelica extract and water to obtain a premix; Step 02), add the yeast fermentation product filtrate to the premix, mix evenly, and obtain a composition containing superoxide dismutase SOD.