Colloidal gold kit clamping shell and colloidal gold kit with same

Abstract

The utility model provides a colloidal gold kit clamping shell and colloidal gold kit with the same, the colloidal gold kit clamping shell comprises an upper clamping shell and a lower clamping shell which are buckled with each other, a sample adding hole and an observation window are formed on the upper clamping shell, a clamping groove for positioning a colloidal gold test strip is formed on the inner side surface of the lower clamping shell, and the upper clamping shell and the lower clamping shell are buckled with each other. The inner side surface of the upper clamping shell can limit the colloidal gold test strip in the clamping groove, the clamping groove is provided with a first groove bottom wall corresponding to the position of the sample adding hole, and the first groove bottom wall is closer to the upper clamping shell along the direction from the sample adding hole to the observation window to form a flow guide slope surface; the gradient of the diversion slope is a, and a is larger than or equal to 5 degrees and smaller than or equal to 10 degrees. According to the utility model, the conditions in the prior art that the chromatography is too fast and the detection line or the quality control line is pasty due to the fact that the sample is too thick and still runs on the plate or the sample is too thin and liquid rushes onto the test paper are effectively avoided.

Description

Technical Field

[0001] The utility model belongs to the field of biotechnology, and in particular relates to a colloidal gold test kit cartridge and a colloidal gold test kit having the same. Background Art

[0002] Colloidal gold immunochromatography technology was developed in the 1980s. Faulk and Taylor introduced colloidal gold into immunochemistry in 1971. Since then, the method has been widely used in many fields, including drug testing and biomedicine. Currently, there are over a hundred commercial colloidal gold test kits available both domestically and internationally, including the most common early pregnancy test strips and gonorrhea test kits.

[0003] Colloidal gold rapid detection technology is an emerging technology currently being applied in a variety of fields, including pesticide residue detection, clinical disease detection, and drug detection. With widespread adoption, the technology is gradually maturing. In clinical testing, commercialized test strips based on colloidal gold immunoassay technology require external cartridges for more effective chromatographic testing.

[0004] High-impact polystyrene (HIPS) resin is a low-cost thermoplastic with excellent processing properties and aesthetically pleasing finishes. The addition of flame retardants and toughening agents effectively creates a highly tough, compressive surface. Plastic cartridges made from this material exhibit strong compressive resistance and excellent toughness, effectively protecting colloidal gold test strips when fabricated into test kit cartridges.

[0005] Currently, there are two types of cartridges on the market. One is for running saliva, urine, nasal / nasopharyngeal / oropharyngeal swab samples; the lower card slot has a slope (slope ≥ 15°); the other is a flat card slot for running whole blood, which has no slope.

[0006] (1) Test kits with a non-sloped slot can only be used to run thick samples such as whole blood, plasma, and serum. When the sample is chromatographed with this type of cartridge, the sample can be chromatographed smoothly along the horizontal surface under the principle of siphon action; when the sample is chromatographed with this type of cartridge, the liquid can easily flow quickly along the membrane to the other end due to the relatively flat surface, resulting in the T line or C line on the nitrocellulose membrane being too blurred due to excessive chromatographing liquid, affecting the interpretation result.

[0007] (2) Slope groove kit, commonly used for water-like samples or samples treated by diluent, so that the test substance is dissolved in the diluent, and the diluent is detected. Because the groove itself has a certain slope (slope ≥ 15°), the liquid chromatography will also produce a downward gravity in addition to the siphon effect, thereby resisting the siphon force caused by the liquid being too dilute, so that the liquid can be siphoned at a certain speed, not too fast or too slow, thereby not affecting the result interpretation. When this type of groove runs full blood, serum or plasma thick sample, due to the high slope, the blood, serum / plasma cannot have enough force to chromatograph even under the siphon effect. Content of the utility model

[0008] Therefore, the technical problem to be solved by the utility model is to provide a colloidal gold kit cartridge and a colloidal gold kit with the same, which can be used for chromatography of various samples such as saliva, urine, nasal / nasopharyngeal / oropharyngeal swab samples, whole blood, serum, plasma, skin secretions, etc., and will not appear the conditions of running plate immobility due to too thick sample or liquid rushing to the test paper due to too dilute sample, resulting in too fast chromatography and detection line or quality control line blurring.

[0009] In order to solve the above problems, the utility model provides a colloidal gold kit cartridge, which comprises a upper cartridge and a lower cartridge which are buckled to each other, a sample adding hole and an observation window are formed on the upper cartridge, a groove for positioning a colloidal gold test strip is formed on the inner side surface of the lower cartridge, the inner side surface of the upper cartridge can limit the colloidal gold test strip in the groove, the groove has a first groove bottom wall corresponding to the position of the sample adding hole, along the direction from the sample adding hole to the observation window, the first groove bottom wall is closer and closer to the upper cartridge to form a flow guide slope surface, the slope of the flow guide slope surface is a, 5° ≤ a ≤ 10°.

[0010] In some embodiments, along the direction from the sample adding hole to the observation window, the positioning colloidal gold test strip comprises a sample pad section and a nitrocellulose membrane section in sequence, the sample pad section and the nitrocellulose membrane section have an overlapping section, a first horizontal bar and a second horizontal bar are further formed on the inner side surface of the upper cartridge, the first horizontal bar and the second horizontal bar are arranged in the length direction of the overlapping section and correspond to the starting overlapping line and the ending overlapping line of the overlapping section respectively, and the first horizontal bar and the second horizontal bar form a flow gap with the top surface of the overlapping section respectively.

[0011] In some embodiments, the flow gap is d, 0mm < d ≤ 0.1mm.

[0012] In some embodiments, along the direction from the lower cartridge to the upper cartridge, the hole diameter of the sample adding hole is larger and larger.

[0013] In some embodiments, the cross-section of the sample loading hole is elliptical, and the major axis of the ellipse is parallel to the length extension direction of the positioning colloidal gold test strip.

[0014] In some embodiments, the aperture of the observation window becomes larger along the direction from the lower chuck to the upper chuck.

[0015] In some embodiments, the card slot has a test strip support strip located on the inner wall surface of the lower shell, and the guide slope is located at one end of the test strip support strip.

[0016] In some embodiments, the upper housing and the lower housing are connected by a snap-fit ​​interference fit; and / or, the upper housing and the lower housing have an interference fit on their outer peripheral edges.

[0017] In some embodiments, a plurality of pins are spaced apart at the edge of the inner wall surface of the upper housing, and a plurality of hole columns are spaced apart at the edge of the inner wall surface of the lower housing, and each of the pins is interference-fitted into each of the hole columns one by one.

[0018] The utility model also provides a colloidal gold test kit, comprising the colloidal gold test kit cartridge.

[0019] The utility model provides a colloidal gold test kit cartridge and a colloidal gold test kit having the same. A diversion slope is provided at a position corresponding to the sample addition hole, i.e., below the sample pad section of the colloidal gold test strip, and the slope is set between 5° and 10°. The slope is small, and the gravity resistance generated by the slope is insufficient to resist the force generated by the siphon effect. Therefore, whether it is a whole blood sample or an ordinary sample, both can be chromatographed to the terminal without affecting the result reading of the test strip (i.e., the aforementioned reagent strip). At the same time, for a relatively thin sample such as water, the slope does not chromatograph to the terminal point of the test strip instantaneously due to the existence of the small slope, but chromatographs to the terminal point at a certain speed, thereby not affecting the result reading of the test strip. This effectively avoids the problem in the prior art that the sample is too thick and the plate does not move, or the sample is too thin and the liquid rushes onto the test strip, resulting in too fast chromatography and blurred detection lines or quality control lines. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram (cross-sectional view) of the internal structure of the colloidal gold test kit cartridge of an embodiment of the present utility model;

[0021] Figure 2 for Figure 1 A half-section view of the colloidal gold test kit cartridge from a top-down perspective;

[0022] Figure 3 for Figure 1 The front view of the upper casing (from the inside perspective);

[0023] Figure 4 for Figure 3 Cross-section of the middle AA;

[0024] Figure 5 for Figure 1 The front view of the lower cassette (from the inner side);

[0025] Figure 6 for Figure 5 Cross-section of the BB.

[0026] The reference numerals indicate:

[0027] 1. Upper housing; 11. Sample loading hole; 12. Observation window; 131. First horizontal bar; 132. Second horizontal bar; 2. Lower housing; 21. Slot; 211. Bottom wall of first slot; 22. Test strip support bar; 31. Pin; 32. Hole column. DETAILED DESCRIPTION

[0028] See also Figures 1 to 6 As shown, according to an embodiment of the present utility model, a colloidal gold test kit cartridge is provided, comprising an upper cartridge 1 and a lower cartridge 2 that are engaged with each other, wherein the upper cartridge 1 is formed with a sample addition hole 11 and an observation window 12, and the inner side surface of the lower cartridge 2 is formed with a slot 21 for positioning a colloidal gold test strip, wherein the inner side surface of the upper cartridge 1 can limit the colloidal gold test strip (not shown in the figure) in the slot 21, and the slot 21 has a first groove bottom wall 211 corresponding to the position of the sample addition hole 11, It can be understood that the colloidal gold test strip is confined in the aforementioned card slot 21, the position of its sample pad section corresponds to the position of the sample addition hole 11 in the upper and lower directions, and at least the positions of the C line (quality control line) and the T line (detection line) of its nitrocellulose membrane section are located in the aforementioned observation window 12. Along the direction from the sample addition hole 11 to the observation window 12, the bottom wall 211 of the first groove gets closer and closer to the upper card shell 1 to form a guide slope, and the slope of the guide slope is a, 5°≤a≤10°.

[0029] In this technical solution, the slope of the diversion slope is set at the position corresponding to the sample addition hole 11, that is, at the position below the sample pad section of the colloidal gold test strip, and is set to be between 5° and 10°. The slope is relatively small, and the gravitational resistance generated by the slope is insufficient to resist the force generated by the siphon effect. Therefore, whether it is a whole blood sample or an ordinary sample, it can be chromatographed to the terminal without affecting the result reading of the test strip (that is, the aforementioned test strip). At the same time, for a relatively thin sample such as water, due to the existence of the small slope, the sample will not be chromatographed to the end point of the test strip instantaneously, but will be chromatographed to the end point at a certain speed, thereby not affecting the result reading of the test strip. This effectively avoids the situation in the prior art where the sample is too thick and the plate does not move, or the sample is too thin and the liquid rushes onto the test paper, resulting in too fast chromatography and blurred detection lines or quality control lines.

[0030] In some embodiments, along the direction from the sample loading hole 11 to the observation window 12, as described above, the positioning colloidal gold test strip includes a sample pad segment and a nitrocellulose membrane segment (not shown in the figure) in sequence, and the sample pad segment and the nitrocellulose membrane segment have an overlapping section. A first horizontal bar 131 and a second horizontal bar 132 are also formed on the inner side surface of the upper card shell 1. The first horizontal bar 131 and the second horizontal bar 132 are arranged at intervals along the length direction of the overlapping section, and are respectively arranged corresponding to the starting overlap line and the end overlap line of the overlapping section. The first horizontal bar 131 and the second horizontal bar 132 respectively form a flow gap with the top surface of the overlapping section. In a specific embodiment, the flow gap is d, 0mm<d≤0.1mm.

[0031] In this technical solution, after the test strip is assembled in the slot 21 of the aforementioned test kit cartridge, a small flow gap is formed between the top surface of the test strip and the upper cartridge 1, so that the sample solution passing through forms a relatively thin layer, thereby ensuring that both thick samples and relatively dilute water samples can be chromatographed on the nitrocellulose membrane section at a certain speed, further eliminating the situation where the test line or quality control line becomes blurred due to excessive chromatography.

[0032] In some embodiments, the diameter of the sample loading hole 11 increases along the direction from the lower housing 2 to the upper housing 1 .

[0033] In this technical solution, the sample addition hole 11 is objectively a structure that expands toward the user operation side, which can effectively prevent the contamination caused by the outflow of sample liquid due to the difficulty in aligning the sample solution with the sample addition hole 11 when the user adds the sample, that is, it effectively prevents the contamination of the loaded sample and prevents the sample from contaminating the user and the surrounding environment such as the testing table during the detection process.

[0034] In a specific embodiment, the cross section of the sample addition hole 11 is elliptical, and the major axis of the ellipse is parallel to the length extension direction of the positioning colloidal gold test strip.

[0035] In another preferred embodiment, the aperture of the observation window 12 becomes larger and larger along the direction from the lower chuck 2 to the upper chuck 1. In a specific embodiment, the observation window is an inverted frustum hole structure.

[0036] In order to ensure the flatness of the test strip during use, in some embodiments, the slot 21 has a test strip support strip 22 on the inner wall surface of the lower card shell 2, and the guide slope is at one end of the test strip support strip 22. The aforementioned test strip support strip 22 is supported on the bottom side of the test strip, and its top side remains well flat under the compression and clamping action of the upper card shell 1.

[0037] The upper card shell 1 and the lower card shell 2 are connected by a snap-on interference fit. Specifically, a plurality of pins 31 are arranged at intervals on the edge position of the inner wall surface of the upper card shell 1, and a plurality of hole columns 32 are arranged at intervals on the edge position of the inner wall surface of the lower card shell 2. Each of the pins 31 is interference-fitted into each of the hole columns 32 in a one-to-one correspondence, thereby realizing quick assembly of the upper card shell 1 and the lower card shell 2. The simple structure can also reduce production costs.

[0038] In another preferred embodiment, the outer peripheral edges of the upper card shell 1 and the lower card shell 2 are interference fit. For example, the outer edge mating surface of the upper card shell 1 is an annular groove, and the outer edge mating surface of the lower card shell 2 is an annular platform. The interference fit between the annular platform and the annular groove can form a relatively sealed space between the upper card shell 1 and the lower card shell 2, thereby improving the sealing performance of the card shell of the utility model.

[0039] The upper housing 1 and the lower housing 2 are both integrally injection-molded from high-impact polystyrene (HIPS) resin in the prior art and then assembled together, thereby having strong compressive resistance and toughness.

[0040] According to an embodiment of the present invention, a colloidal gold kit is further provided, comprising the above-mentioned colloidal gold kit cartridge.

[0041] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0042] The above merely is the preferred embodiment of the present application, and does not limit the present application, and any modification, equivalent replacement and improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application. The above is merely the preferred embodiment of the present application, and it should be pointed out that, for ordinary skilled in the art, without departing from the technical principle of the present application, a number of improvements and modifications can be made, and these improvements and modifications shall be considered as the protection scope of the present application.

Claims

1. A colloidal gold test kit stuck, characterized in that: The invention comprises an upper card shell (1) and a lower card shell (2) which are engaged with each other, wherein the upper card shell (1) is formed with a sample addition hole (11) and an observation window (12), and the inner side surface of the lower card shell (2) is formed with a card groove (21) for positioning a colloidal gold test strip, and the inner side surface of the upper card shell (1) can limit the colloidal gold test strip to be located in the card groove (21), and the card groove (21) has a first groove bottom wall (211) corresponding to the position of the sample addition hole (11), and along the direction from the sample addition hole (11) to the observation window (12), the first groove bottom wall (211) is increasingly close to the upper card shell (1) to form a guide slope, and the slope of the guide slope is a, 5°≤a≤10°.

2. The colloidal gold test kit according to claim 1, wherein: Along the direction from the sample loading hole (11) to the observation window (12), the positioning colloidal gold test strip includes a sample pad section and a nitrocellulose membrane section in sequence, and the sample pad section and the nitrocellulose membrane section have an overlapping section. A first horizontal bar (131) and a second horizontal bar (132) are also formed on the inner side surface of the upper card shell (1). The first horizontal bar (131) and the second horizontal bar (132) are arranged at intervals along the length direction of the overlapping section and are respectively arranged corresponding to the starting overlapping line and the end overlapping line of the overlapping section. The first horizontal bar (131) and the second horizontal bar (132) respectively form a flow gap with the top surface of the overlapping section.

3. The colloidal gold test kit according to claim 2, wherein: The flow gap is d, 0mm<d≤0.1mm.

4. The colloidal gold test kit according to claim 1, wherein: Along the direction from the lower housing (2) to the upper housing (1), the aperture of the sample addition hole (11) becomes larger and larger.

5. The colloidal gold test kit according to claim 4, characterized in that: The cross section of the sample addition hole (11) is elliptical, and the major axis of the ellipse is parallel to the length extension direction of the positioning colloidal gold test strip.

6. The colloidal gold test kit according to claim 1, characterized in that: Along the direction from the lower chuck (2) to the upper chuck (1), the aperture of the observation window (12) becomes larger and larger.

7. The colloidal gold test kit according to claim 1, characterized in that: The card slot (21) has a test strip support strip (22) located on the inner wall surface of the lower card shell (2), and the guide slope is located at one end of the test strip support strip (22).

8. The colloidal gold test kit according to claim 1, characterized in that: The upper card shell (1) and the lower card shell (2) are connected by a snap-fit ​​interference fit; and / or, the outer peripheral edges of the upper card shell (1) and the lower card shell (2) are interference-fitted.

9. The colloidal gold test kit according to claim 8, characterized in that: A plurality of clamping pins (31) are arranged at intervals on the edge of the inner wall surface of the upper clamping shell (1), and a plurality of hole columns (32) are arranged at intervals on the edge of the inner wall surface of the lower clamping shell (2), and each of the clamping pins (31) is interference-inserted into each of the hole columns (32) in a one-to-one correspondence.

10. A colloidal gold kit, characterized in that: The colloidal gold test kit cartridge comprises the colloidal gold test kit cartridge according to any one of claims 1 to 9.

Images