Oligonucleotide probe kit for detecting common intestine trac kpathogenic bacteria and its use

An oligonucleotide probe, oligonucleotide technology, applied in the determination/inspection of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc., can solve problems such as inability to achieve
CN1683565AInactive Publication Date: 2005-10-19RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA
Publication Date
2005-10-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention belongs to the field of microbe detecting technology. The oligonucleotide probe for detecting common intestinal tract pathogenic bacteria is designed on 16S rRNA and 23S rRNA of bacteria, ipaH of dysentery bacillus giant plasmid, VipR of Salmonella typhi and other gene sequence, has length of 25-50 bp, and relatively high sensitivity and specificity. The oligonucleotide probe is suitable for detection based on nucleic acid hybridization principle, especially detection based on gene chip principle. Under certain use condition, it can detect Listeria, parahemolutic vibrio, Campylobacter, etc. It may be used in many aspects, such as disease diagnosis, environment detection, food poisoning detection, etc.
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Description

technical field

[0001] The invention belongs to the technical category of microorganism detection technology, especially the nucleic acid detection technology of intestinal pathogenic bacteria. The invention relates to a set capable of detecting Listeria, Vibrio parahaemolyticus, Campylobacter, Yersinia enterocolitica, Yersinia, Staphylococcus aureus, Clostridium botulinum, wax sample Oligonucleotide detection of Bacillus, Salmonella, Salmonella typhi, Shigella, Shigella, Clostridium perfringens, Proteus, Brucella, EHE O157:H7 and Vibrio cholerae needle, the sequence and use of the set of probes. Background technique

[0002] Enteropathogenic bacteria are the main pathogens that cause infectious diseases with symptoms of diarrhea and vomiting clinically. Common pathogens include Listeria, Vibrio parahaemolyticus, Campylobacter, Yersinia enterocolitica, Yersinia spp, Staphylococcus aureus, Clostridium botulinum, Bacillus cereus 17 species, including Salmonella, Salmonella ...

Claims

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