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Brucella live vaccine and production method thereof

A Brucella and production method technology, applied in the field of Brucella live vaccine and its production, can solve the problems of biological safety hazards restricting vaccine development and other issues

Inactive Publication Date: 2012-01-25
CHINA INST OF VETERINARY DRUG CONTROL
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Problems solved by technology

[0006] Although it has been reported that recombinant Brucella crassa was obtained by inserting a resistance gene, the potential biosafety hazards caused by the resistance gene limit the possibility of its development as a vaccine

Method used

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  • Brucella live vaccine and production method thereof
  • Brucella live vaccine and production method thereof
  • Brucella live vaccine and production method thereof

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Embodiment Construction

[0016] The gene involved in the present invention is the Brucella wboA gene related to the smooth phenotype.

[0017] The gene recombination technology involved in the present invention includes: using a shuttle plasmid containing a sucrose sensitive gene as a mediator to screen recombinant Brucella bacteria with wboA gene deletion.

[0018] In the present invention, through two homologous recombination, the sequence between 1-897bp of the wboA gene of the smooth Brucella suis S2 strain is seamlessly deleted, and finally the rough recombinant Brucella suis is obtained by screening rS2 strain (this strain has been sent to the General Microorganism Collection Center of the Chinese Academy of Sciences, Institute of Microbiology, Chinese Academy of Sciences, No. 1, Beichen West Road, Chaoyang District, Beijing, on September 2, 2011, for preservation, and the preservation number is CGMCC No.5212) .

[0019] 1. Construction and screening of recombinant Brucella suis

[0020] The p...

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Abstract

The invention relates to brucella and a production method of a brucella vaccine. In the vaccine, a recombinant brucella rS2-deltaWboA strain is taken as a production strain. A smooth recombinant strain is transformed into a rough recombinant strain by deleting base sequences among WboA genes 1-897bp of a brucella S2 strain and eliminating the forming condition of an O chain in a smooth brucella cell wall LPS (Lipopolysaccharide) structure with a non-resistant gene screening technology. The safety of the rough recombinant strain is improved remarkably, and a good immune effect on brucelliasis is still kept. Due to the adoption of the strain to the production of the vaccine, the current situation that a brucella vaccine immune animal cannot be distinguished from a wild strain infected animal easily is changed, and the safety of the conventional vaccine is effectively improved.

Description

Technical field [0001] The invention relates to a Brucella live vaccine and a production method thereof, belonging to the field of veterinary biological products. technical background [0002] Brucellosis (brucellosis) is a zoonotic disease characterized by abortion and fever caused by Brucella or Brucella, which seriously threatens the life and health of humans and various animals. . This disease not only has serious harm to the reproduction and production performance of animals, but more importantly, people are often difficult to cure after being infected with Brucella, thus causing serious public health problems. Elimination of brucellosis has therefore been one of the most important goals of public health programs in countries where Brucella is endemic. At present, the main method to eliminate the disease worldwide is the combination of culling and immunization. For China, where the occurrence of brucellosis is relatively severe, due to the high cost of culling, vaccin...

Claims

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Application Information

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IPC IPC(8): A61K39/10C12N1/21A61P31/04C12R1/01
Inventor 丁家波陈光华毛开荣王楠程君生周桂兰冯忠武蒋玉文
Owner CHINA INST OF VETERINARY DRUG CONTROL
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