Method for planting Ganoderma Lucidum

A cultivation method and technology of Ganoderma lucidum, applied in the field of cultivation of Ganoderma lucidum, can solve the problems of low content of biologically active substances, scarcity of wild resources, obstacles to mass production of Ganoderma lucidum, etc., and achieve the effect of increasing content and improving activity

Inactive Publication Date: 2016-06-15
GUILIN DA YE LING YVU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fruiting bodies (or sclerotia) produced by traditional field collection or artificial cultivation are scarce in wild resources, the fruiting body cultivation and production cycle is long (4-5 months...

Method used

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  • Method for planting Ganoderma Lucidum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Strain selection: use the tissue separation method to obtain the pure mother species, and use 75v% alcohol to sterilize the surface of a part of the wild or artificially growing fresh Hanzhi fruiting body, then cut it into 3~ For small pieces of 5 mm, take about 5 pieces and place them on the plate medium, or take 1 piece and place them on the test tube slant medium. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.

[0016] 2) Culture medium preparation: the following raw materials in parts by weight: 30 parts of miscellaneous sawdust, 10 parts of corncobs, 5 parts of rice bran, 1 part of burdock seed, 2 parts of Gastrodia elata and 0.5 part of lactose; mix and pulverize the above raw materials, add wat...

Embodiment 2

[0021] 1) Strain selection: use the tissue separation method to obtain the pure mother species, use 75v% alcohol to sterilize a part of the wild or artificially growing fresh fruiting body of Chizhi lucidum, and then cut it into 3 parts by aseptic operation. For small pieces of ~5mm, take about 5 pieces and place them on the plate medium, or take 1 piece and place them on the slant medium of the test tube. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.

[0022] 2) The following raw materials in parts by weight: 35 parts of miscellaneous sawdust, 20 parts of corncobs, 10 parts of rice bran, 3 parts of burdock seeds, 4 parts of Gastrodia elata and 1.5 parts of lactose; mix and pulverize the above raw materials, a...

Embodiment 3

[0027] 1) Strain selection: use the tissue separation method to obtain the pure mother species, and use 75v% alcohol to sterilize the surface of a part of the wild or artificially growing fresh sweet sesame fruiting body, and cut it into pieces by aseptic operation. For small pieces of 3-5 mm, take about 5 pieces and place them on the medium plate, or take 1 piece and place them on the slant medium of the test tube. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.

[0028] 2) The following raw materials in parts by weight: 32 parts of miscellaneous wood chips, 15 parts of corncobs, 8 parts of rice bran, 2 parts of burdock seeds, 3 parts of Gastrodia elata and 1 part of lactose; mix and pulverize the above raw mat...

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Abstract

The invention provides a method for planting Ganoderma Lucidum. The method comprises: strain culture, culture medium preparation, mycelia culture, and sprouting management. The culture medium preparation includes mixing, grinding, sacking, and sterilizing the following raw materials by part of weight: 30-35 mixed sawdust by parts of weight, 10-20 corncob by parts of weight, 5-10 rice bran by parts of weight, 1-3 burdock by parts of weight, 2-4 gastrodia elata by parts of weight, and 0.5-1.5 lactose by parts of weight. The adding of gastrodia elata, burdock, and lactose to the regular culture medium can increase activity of [alpha]-Phosphoglucomutase, promote metabolism of Ganoderma Lucidum cells to migrate more towards a branch synthesized by Ganoderma Lucidum polysaccharide, which greatly increases content of Ganoderma Lucidum exopolysaccharide, and at the same time promotes growth of Ganoderma Lucidum, and greatly shortens time for sacking mycelia.

Description

technical field [0001] The invention relates to the technical field of cultivation of ganoderma, in particular to a cultivation method of ganoderma. Background technique [0002] Ganoderma lucidum (Ganodermalucidum), commonly known as Ganoderma lucidum, is a precious medicinal fungus. It has a high medicinal value, has a health care effect on the human body, and has a preventive, therapeutic or inhibitory effect on diseases. The fruiting body (or sclerotium) produced by traditional field collection or artificial cultivation is scarce in wild resources, the fruiting body cultivation and production cycle is long (4-5 months), production is controlled by climate and other conditions, and the content of biologically active substances is low and many other factors Restrictions have hindered the mass production of Ganoderma lucidum. Ganoderma lucidum polysaccharide is the main effective chemical substance of Ganoderma lucidum, which is a kind of homopolysaccharide or heteropolys...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05F11/00
CPCA01G18/00C05F11/00
Inventor 蒋晓艳
Owner GUILIN DA YE LING YVU BIOTECH CO LTD
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