The invention belongs to the technical field of bacterium cultivation, and discloses a bag cultivation method for inserting a tube into the middle of a shiitake mushroom short bag for inoculating ripematerials. A bag body and a hollow tube are arranged; the bag body is filled with culture materials, a circular hole is formed in the middle of the culture materials, one end of the hollow tube is filled with cotton, the hollow tube is inserted into the circular hole of the bag body, and the end filled with the cotton faces upwards. Accordingly, by punching the middle of the material bag and inserting the tube, in the sterilization process, high pressure steam penetrates through the material bag conveniently, the disinfection and sterilization time is short, sterilization is conducted thoroughly easily, the infection rate is lowered, bacteria inoculated in the middle make contact with the center of the bag materials, heat preservation and moisture retention are achieved, the cotton is good in air permeability, growth oxygen supply is facilitated, the bacteria germinate rapidly, spawn runing is good, and spread and growth in the materials are rapid; the sterilized cotton blocks miscellaneous bacteria invasive infection, and culture period pollution is reduced. For intermediate inoculation, compared with single end short bag inoculation and long bag peripheral punching or nail inoculation, the hypha bagfull time is shortened, long bag overgrowing time needs 60-90 d, and overgrowing of short bag intermediate inoculation only needs 25-30 d.