Lucid ganoderma cultivation method for promoting mycelial growth

A cultivation method and mycelia growth technology, applied in the cultivation field of Ganoderma lucidum, can solve problems such as low content of biologically active substances, scarcity of wild resources, obstacles to mass production of Ganoderma lucidum, etc., and achieve the effect of increasing activity and content

Inactive Publication Date: 2016-06-08
GUILIN DA YE LING YVU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fruiting bodies (or sclerotia) produced by traditional field collection or artificial cultivation are scarce in wild resources, the fruiting body cultivation and production cycle is long (4-5 months...

Method used

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  • Lucid ganoderma cultivation method for promoting mycelial growth

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Strain selection: use the tissue separation method to obtain the pure mother species, and use 75v% alcohol to sterilize the surface of a part of the wild or artificially growing fresh Hanzhi fruiting body, then cut it into 3~ For small pieces of 5 mm, take about 5 pieces and place them on the plate medium, or take 1 piece and place them on the test tube slant medium. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.

[0017] 2) Culture medium preparation: the following raw materials in parts by weight: 30 parts of miscellaneous wood chips, 10 parts of corn cobs, 5 parts of rice bran, 1 part of burdock fruit, 2 parts of grifola frondosa and 0.5 part of lactose; mix and pulverize the above raw materials, a...

Embodiment 2

[0022] 1) Strain selection: use the tissue separation method to obtain the pure mother species, use 75v% alcohol to sterilize a part of the wild or artificially growing fresh fruiting body of Chizhi lucidum, and then cut it into 3 parts by aseptic operation. For small pieces of ~5mm, take about 5 pieces and place them on the plate medium, or take 1 piece and place them on the slant medium of the test tube. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.

[0023] 2) The following raw materials in parts by weight: 35 parts of miscellaneous sawdust, 20 parts of corncobs, 10 parts of rice bran, 3 parts of burdock fruit, 4 parts of frondosa frondosa and 1.5 parts of lactose; mix and pulverize the above raw materials,...

Embodiment 3

[0028] 1) Strain selection: use the tissue separation method to obtain the pure mother species, and use 75v% alcohol to sterilize the surface of a part of the wild or artificially growing fresh sweet sesame fruiting body, and cut it into pieces by aseptic operation. For small pieces of 3-5 mm, take about 5 pieces and place them on the medium plate, or take 1 piece and place them on the slant medium of the test tube. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.

[0029] 2) The following raw materials in parts by weight: 32 parts of miscellaneous wood chips, 15 parts of corncobs, 8 parts of rice bran, 2 parts of burdock fruit, 3 parts of frondosa frondosa and 1 part of lactose; mix and pulverize the above raw m...

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Abstract

The invention provides a lucid ganoderma cultivation method for promoting mycelial growth. The lucid ganoderma cultivation method comprises strain culture, culture medium preparation, mycelium culture and ganoderma lucidum growth management, wherein the culture medium preparation comprises mixing, smashing, bagging and sterilizing the following raw materials in parts by weight: 30 to 35 parts of weed tree sawdust, 10 to 20 parts of corncobs, 5 to 10 parts of rice bran, 1 to 3 parts of burdock, 2 to 4 parts of grifola frondosa and 0.5 to 1.5 parts of milk sugar. According to the lucid ganoderma cultivation method, the grifola frondosa, burdock and milk sugar are added into a conventional culture medium, the activity of alpha-glucophosphomutase can be improved, and branch migration of lucid ganoderma cell metabolism to ganoderan synthesis preferably is promoted, so that the content of ganoderma exopolysaccharides is greatly increased; meanwhile, the growth of lucid ganoderma can be promoted, and the hypha bagful time is greatly shortened.

Description

technical field [0001] The invention relates to the technical field of ganoderma cultivation, in particular to a ganoderma cultivation method for promoting the growth of hyphae. Background technique [0002] Ganoderma lucidum (Ganodermalucidum), commonly known as Ganoderma lucidum, is a precious medicinal fungus. It has a high medicinal value, has a health care effect on the human body, and has a preventive, therapeutic or inhibitory effect on diseases. The fruiting body (or sclerotium) produced by traditional field collection or artificial cultivation is scarce in wild resources, the fruiting body cultivation and production cycle is long (4-5 months), production is controlled by climate and other conditions, and the content of biologically active substances is low and many other factors Restrictions have hindered the mass production of Ganoderma lucidum. Ganoderma lucidum polysaccharide is the main effective chemical substance of Ganoderma lucidum, which is a kind of homo...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F11/00A01G1/04
CPCC05G3/00A01G18/00C05F11/00
Inventor 蒋晓艳
Owner GUILIN DA YE LING YVU BIOTECH CO LTD
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