Preparation method of lentinusedodes liquid strain and matched production method of lentinusedodes stick
A technology of liquid strains and production methods, applied in botany equipment and methods, mushroom cultivation, horticulture, etc., can solve problems such as unsatisfactory quality of liquid strains, incomplete culture medium, long fermentation cycle, etc.
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[0018] A production method for making liquid strains of shiitake mushrooms and high-quality environment-friendly bacteria sticks comprises the following steps:
[0019] (1) Preparation of liquid culture medium for secondary shiitake mushrooms: the components by mass percentage are as follows: 10% corn flour, 6% brown sugar, 2% glucose, 1% bran, 0.5% potassium dihydrogen phosphate, 0.2% Magnesium Sulfate Heptahydrate, 0.1% Edible Oil, VB 1 10mg, the balance is distilled water, and dilute to 1000mL;
[0020] (2) Sterilization and inoculation of secondary liquid strains: sterilize at 121°C for more than 60 minutes, cool to 25°C, and insert the mother species of shiitake mushrooms;
[0021] (3) Cultivation of secondary liquid strains: After adding shiitake mushroom strains, culture them in the dark at 25°C and 110r / min in a constant temperature shaker, and obtain secondary liquid strains with uniform bacterial ball size and clear medium within 5 days; liquid It takes only 5 days...
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