Preparation method of lentinusedodes liquid strain and matched production method of lentinusedodes stick

A technology of liquid strains and production methods, applied in botany equipment and methods, mushroom cultivation, horticulture, etc., can solve problems such as unsatisfactory quality of liquid strains, incomplete culture medium, long fermentation cycle, etc.

Pending Publication Date: 2021-05-18
GUIZHOU UNIV
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Problems solved by technology

At present, the production technology of liquid strains of shiitake mushrooms is becoming more and more mature. Liquid strains have a short production cycle and uniform age. Using them to inoculate solid cultivation materials has the advantages of fast flow, easy dispersion, fast germination, and fast planting. However, liquid strains of shiitake mushrooms There are problems such as incomplete culture medium, long fermentation period, and unsatisfactory quality of the obtained liqu

Method used

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Embodiment Construction

[0018] A production method for making liquid strains of shiitake mushrooms and high-quality environment-friendly bacteria sticks comprises the following steps:

[0019] (1) Preparation of liquid culture medium for secondary shiitake mushrooms: the components by mass percentage are as follows: 10% corn flour, 6% brown sugar, 2% glucose, 1% bran, 0.5% potassium dihydrogen phosphate, 0.2% Magnesium Sulfate Heptahydrate, 0.1% Edible Oil, VB 1 10mg, the balance is distilled water, and dilute to 1000mL;

[0020] (2) Sterilization and inoculation of secondary liquid strains: sterilize at 121°C for more than 60 minutes, cool to 25°C, and insert the mother species of shiitake mushrooms;

[0021] (3) Cultivation of secondary liquid strains: After adding shiitake mushroom strains, culture them in the dark at 25°C and 110r / min in a constant temperature shaker, and obtain secondary liquid strains with uniform bacterial ball size and clear medium within 5 days; liquid It takes only 5 days...

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Abstract

The invention relates to a preparation method of a lentinusedodes liquid strain and a matched production method of a lentinusedodes stick. The preparation method comprises the following specific steps: (1) preparing a culture medium for a secondary liquid strain; (2) sterilizing and inoculating the secondary liquid strain; (3) culturing the secondary liquid strain; (4) preparing a cultivation material for a third-level solid high-quality lentinusedodes stick; (5) sterilizing and inoculating the third-level solid high-quality lentinusedodes stick; and (6) culturing the third-level solid high-quality lentinusedodes stick. According to the method, a brand new strain formula is used for culturing the secondary liquid strain of the lentinusedodes, the method has the advantages of short culture time, high purity, strong vitality and the like, the survival rate after inoculation is high, the spawn running speed is high, the culture time of the third-level solid high-quality lentinusedodes stick can be shortened, the labor cost is reduced, and the yield and quality of the shiitake mushrooms can be improved.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a method for producing liquid strains of shiitake mushrooms and a supporting production method of high-quality and environment-friendly fungus sticks. Background technique [0002] mushroom( Lentinusedodes ) belonging to the order Agaricaceae ( Agaricales ) Tricholomaceae ( Tricholomatacete ) Lentinus ( Lentinus ), is the second largest edible fungus in the world, one of the bulk edible fungi in my country, and is a famous edible and medicinal fungus. Shiitake mushrooms are rich in nutrition, delicious in taste and pleasant in fragrance. They are rich in essential amino acids, accounting for about 35.7% of the total amount of amino acids. At present, the production technology of liquid strains of shiitake mushrooms is becoming more and more mature. Liquid strains have a short production cycle and uniform age. Using them to inoculate solid cultivation materials has ...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/30A01G18/50
CPCA01G18/20A01G18/50A01G18/30
Inventor 徐秀红张万萍陈阿敏裴芸徐彦军李伟文庭池张健
Owner GUIZHOU UNIV
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