Method for cultivating lucid ganoderma by using traditional Chinese medicine
A technology of Ganoderma lucidum and traditional Chinese medicine, which is applied in cultivation, mushroom cultivation, plant cultivation, etc. It can solve the problems of long fruit body cultivation production cycle, low content of biologically active substances, and obstacles to mass production of Ganoderma lucidum, so as to promote the growth of Ganoderma lucidum, improve activity, The effect of shortening the full bag time
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Embodiment 1
[0016] 1) Strain selection: use the tissue separation method to obtain the pure mother species, and use 75v% alcohol to sterilize the surface of a part of the wild or artificially growing fresh Hanzhi fruiting body, then cut it into 3~ For small pieces of 5 mm, take about 5 pieces and place them on the plate medium, or take 1 piece and place them on the test tube slant medium. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.
[0017] 2) Culture medium preparation: the following raw materials in parts by weight: 30 parts of miscellaneous sawdust, 10 parts of corncobs, 5 parts of rice bran, 1 part of Grifola frondosa and 2 parts of Gastrodia elata; mix and pulverize the above raw materials, add water until the mois...
Embodiment 2
[0022] 1) Strain selection: use the tissue separation method to obtain the pure mother species, use 75v% alcohol to sterilize a part of the wild or artificially growing fresh fruiting body of Chizhi lucidum, and then cut it into 3 parts by aseptic operation. For small pieces of ~5mm, take about 5 pieces and place them on the plate medium, or take 1 piece and place them on the slant medium of the test tube. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.
[0023] 2) The following raw materials in parts by weight: 35 parts of miscellaneous wood chips, 20 parts of corncobs, 10 parts of rice bran, 3 parts of Grifola frondosa and 4 parts of Gastrodia elata; mix and pulverize the above raw materials, add water until t...
Embodiment 3
[0028] 1) Strain selection: use the tissue separation method to obtain the pure mother species, and use 75v% alcohol to sterilize the surface of a part of the wild or artificially growing fresh sweet sesame fruiting body, and cut it into pieces by aseptic operation. For small pieces of 3-5 mm, take about 5 pieces and place them on the medium plate, or take 1 piece and place them on the slant medium of the test tube. Cultivate at 25-28°C for 3-4 days. When white hyphae grow around the small tissue, select pure white hyphae and transplant them to a new slant medium, and continue to cultivate for about 5 days to obtain Ganoderma lucidum mother. species, and then expanded and cultivated into original species and cultivated species.
[0029] 2) The following raw materials in parts by weight: 32 parts of miscellaneous wood chips, 15 parts of corncobs, 8 parts of rice bran, 2 parts of Grifola frondosa and 3 parts of Gastrodia elata; mix and pulverize the above raw materials, add wate...
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