Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving phellinus igniarius cultivation efficiency through laboratory intelligent cultivation management

A cultivation management and laboratory technology, applied in the field of laboratory intelligent cultivation management to improve Phellinus linteus cultivation efficiency, can solve the problems of underutilization, low Phellinus linteus quality, short cultivation period, etc. Effect of Phellinus linteus quality and short cultivation period

Active Publication Date: 2021-04-09
湖南省蚕桑科学研究所
View PDF12 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Phellinus flavone content cultivated by its disclosed technical scheme is only about 2.7%, and the Phellinus flavonoids obtained are of lower quality.
[0009] In addition, about 100-200 billion tons of plant organic matter are formed every year in the world, half of which are cellulose substances. In my country, only about 700 million tons of crop residues are formed in agricultural production every year. These wastes have not been fully utilized. polluted environment
[0010] Therefore, it is urgent to provide a kind of Phellinus japonica high-efficiency cultivation technology that develops a short cultivation period, can be standardized, industrialized, and intelligently produced, effectively solves the pollution problem in the cultivation process, and keeps the product quality stable, so that the production cost is greatly reduced. technical issues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving phellinus igniarius cultivation efficiency through laboratory intelligent cultivation management
  • Method for improving phellinus igniarius cultivation efficiency through laboratory intelligent cultivation management
  • Method for improving phellinus igniarius cultivation efficiency through laboratory intelligent cultivation management

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0048] Optimization of the quality improvement of the solid plate medium of the Phellinus radix parent species: under sterile conditions, after inoculating the activated Phellinus slant parent species on the solid plate medium of the Phellinus chinensis species, cultured at 26°C for 10 days to optimize the quality. Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0049] The components used in the solid plate medium are: 230g / L potato, 23g / L glucose, 0.3g / L lignin, 1.2g / LMgSO 4 , 1.5g / LKH 2 PO 4 , 1.5ml / L wood vinegar, 18.0g / L agar, the remainder water, and adjust the pH to 6.0.

[0050] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark c...

Embodiment 3

[0057] Optimization of the quality improvement of the solid plate medium of the Phellinus radix parent species: under sterile conditions, after inoculating the activated Phellinus slant parent species on the solid plate medium of the Phellinus chinensis species, cultured at 26°C for 10 days to optimize the quality. Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0058] The ingredients used in the solid plate medium are: 200g / L potato, 24g / L glucose, 0.2g / L lignin, 1.1g / LMgSO 4 , 1.4g / LKH 2 PO 4 , 2.0ml / L wood vinegar, 17.0g / L agar, the balance of water, and adjust the pH to 6.0.

[0059] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark...

Embodiment 4

[0067] Optimization of the quality improvement of the solid plate medium of Phellinus chinensis: Under sterile conditions, after inoculating the activated Phellinus slant mother species on the solid plate medium of Phellinus chinensis, cultured at 25°C for 10 days to improve the quality, select Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0068] The ingredients used in the solid plate medium are: 220g / L potato, 20g / L glucose, 0.4g / L lignin, 1.3g / LMgSO 4 , 1.6g / LKH 2 PO 4 , 3.0ml / L wood vinegar, 16.0g / L agar, the balance of water, and adjust the pH to 6.0.

[0069] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark conditions, the temp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving phellinus igniarius cultivation efficiency through laboratory intelligent cultivation management. The method comprises the steps of quality improvement and optimization of a phellinus igniarius mother strain solid plate culture medium, intermittent propagation culture of a phellinus igniarius liquid strain, optimization of the culture medium through a mulberry twig bag material, intelligent spawn running, fruiting cultivation management and the like. According to the method, the culture period of mycelium and sporophore of phellinus igniarius is short, the biotransformation efficiency is high, and the content of bioactive components such as polysaccharide and triterpene is high. According to the method, laboratory intelligent management is remotely controlled through a mobile phone, cultivation conditions are remotely, flexibly and accurately controlled in an all-weather mode, and the hypha bag filling time is shortened to about 40 days. After the fungus bags are opened, the cultivation period of the sporophore of phellinus igniarius is shortened to about 70 days, compared with a cultivation method in the prior art, the cultivation period is shortened by 60 days or above, and the method achieves high biotransformation efficiency and sporophore yield.

Description

technical field [0001] The invention relates to the technical field of medicinal and edible fungus cultivation, in particular to a method for improving the cultivation efficiency of Phellinus phellidis by intelligent cultivation management in a laboratory. Background technique [0002] Phellinus mulberry is a precious Chinese herbal medicine growing on mulberry trees. It has been used as a traditional Chinese medicine for more than 2,000 years. "Describes Phellinus as "long-term service, making light of life and prolonging life", so Phellinus has the reputation of "forest gold". At present, many institutions in China have preserved Phellinus species and have carried out a series of research. Phellinus anticancer effect was first announced by the Japanese National Cancer Research Center in 1968. The experiment showed that the water extract of the wild Phellinus fruiting body had an inhibitory effect on mouse sarcoma S180 tumor cells, and the proliferation inhibition rate was...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/40A01G18/20
CPCA01G18/00A01G18/40A01G18/20
Inventor 张俊颜新培李一平廖模祥邵元元黄晓强夏伶俐张晓黄仁志叶添梅李霞
Owner 湖南省蚕桑科学研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com