Ganoderma lucidum cultivation method capable of increasing content of ganoderma lucidum polysaccharide

Abstract

The invention provides a ganoderma lucidum cultivation method capable of increasing the content of ganoderma lucidum polysaccharide. The ganoderma lucidum cultivation method comprises the steps of strain cultivation, culture medium preparation, hypha cultivation and ganoderma lucidum emergence management, wherein the culture medium preparation is characterized by mixing, smashing, bagging and sterilizing the following raw materials in parts by weight: 30 to 35 parts of mixed wood chips, 10 to 20 parts of corncobs, 5 to 10 parts of rice bran, 1 to 3 parts of grifola frondosa and 2 to 4 parts of fructus arctii. According to the ganoderma lucidum cultivation method provided by the invention, the fructus arctii and the grifola frondosa are added in a conventional culture medium, so that the activity of alpha-phosphoglucomutase can be increased, the metabolism of ganoderma lucidum cells is facilitated to more migrate towards a branch compounded by the ganoderma lucidum polysaccharide, and the content of exopolysaccharide of ganoderma lucidum can be greatly increased; meanwhile, the growth of the ganoderma lucidum can be promoted, and the full bagging time of hypha is greatly shortened.

Description

Technical field

[0001] The invention relates to the technical field of Ganoderma lucidum cultivation, in particular to a Ganoderma lucidum cultivation method that can increase the content of Ganoderma lucidum polysaccharides. Background technique

[0002] Ganodermalucidum, commonly known as Ganoderma lucidum, is a precious medicinal fungus. It has high medicinal value, has a health care effect on the human body, and has a preventive, therapeutic or inhibitory effect on diseases. The fruit bodies (or sclerotia) collected in the traditional field or artificially cultivated receive many factors such as scarcity of wild resources, long production period of fruit body cultivation (April to May), production controlled by climate and other conditions, and low content of biologically active substances. Restrictions have hindered the mass production of Ganoderma lucidum. Ganoderma lucidum polysaccharide is the main effective chemical substance of Ganoderma lucidum. It is a kind of homop...

Examples

Embodiment 1

[0016] 1) Selection of strains: use the tissue separation method to obtain the pure mother seed, and use 75v% alcohol to disinfect a part of the wild or artificially cultivated fresh Hanzhi fruiting body, and then cut it into 3~ For small pieces of 5mm, take 5 pieces and place them on the plate culture medium, or take one piece and place them on the test tube slope culture medium. Cultivate for 3 to 4 days at 25~28℃. When white hyphae grow around the small tissues, select pure white hyphae and transplant them to a new slant medium. Continue to cultivate for about 5 days to obtain Ganoderma lucidum mother Species, and then expand into the original species and cultivated species.

[0017] 2) Preparation of culture medium: the following parts by weight of raw materials: 30 parts of sawdust, 10 parts of corn cob, 5 parts of rice bran, 1 part of Grifola frondosa, and 2 parts of burdock; the above raw materials are mixed and crushed, and water is added to a moisture content of 60 %, r...

Embodiment 2

[0022] 1) Selection of strains: use the tissue separation method to obtain the pure mother seed, and use 75v% alcohol to disinfect a part of the wild or artificially cultivated fresh Ganoderma lucidum fruit body, and then cut it into 3 by aseptic operation ~5mm small pieces, take about 5 pieces and place them on the plate culture medium, or take one piece and place them on the test tube slope culture medium. Cultivate for 3 to 4 days at 25~28℃. When white hyphae grow around the small tissues, select pure white hyphae and transplant them to a new slant medium. Continue to cultivate for about 5 days to obtain Ganoderma lucidum mother Species, and then expand into the original species and cultivated species.

[0023] 2) The following parts by weight of raw materials: 35 parts of sawdust, 20 parts of corn cob, 10 parts of rice bran, 3 parts of Grifola frondosa, and 4 parts of burdock; mix and pulverize the above raw materials, add water to 65% of water content. Bagging. Each bag of...

Embodiment 3

[0028] 1) Selection of strains: use the tissue separation method to obtain the pure mother seed, and use 75v% alcohol to disinfect a part of the wild or artificially cultivated fresh sweet mushroom fruit bodies, and then cut them into aseptic methods For small pieces of 3 to 5 mm, take 5 pieces and place them on the plate culture medium, or take one piece and place them on the test tube slope culture medium. Cultivate for 3 to 4 days at 25~28℃. When white hyphae grow around the small tissues, select pure white hyphae and transplant them to a new slant medium. Continue to cultivate for about 5 days to obtain Ganoderma lucidum mother Species, and then expand into the original species and cultivated species.

[0029] 2) The following parts by weight of raw materials: 32 parts of sawdust, 15 parts of corn cob, 8 parts of rice bran, 2 parts of Grifola frondosa and 3 parts of burdock; mix and crush the above raw materials, add water to 62% water, and then bag . Each bag of dry materi...

Technical field

[0001] The invention relates to the technical field of Ganoderma lucidum cultivation, in particular to a Ganoderma lucidum cultivation method that can increase the content of Ganoderma lucidum polysaccharides. Background technique

[0002] Ganodermalucidum, commonly known as Ganoderma lucidum, is a precious medicinal fungus. It has high medicinal value, has a health care effect on the human body, and has a preventive, therapeutic or inhibitory effect on diseases. The fruit bodies (or sclerotia) collected in the traditional field or artificially cultivated receive many factors such as scarcity of wild resources, long production period of fruit body cultivation (April to May), production controlled by climate and other conditions, and low content of biologically active substances. Restrictions have hindered the mass production of Ganoderma lucidum. Ganoderma lucidum polysaccharide is the main effective chemical substance of Ganoderma lucidum. It is a kind of homop...

Examples

Embodiment 1

[0016] 1) Selection of strains: use the tissue separation method to obtain the pure mother seed, and use 75v% alcohol to disinfect a part of the wild or artificially cultivated fresh Hanzhi fruiting body, and then cut it into 3~ For small pieces of 5mm, take 5 pieces and place them on the plate culture medium, or take one piece and place them on the test tube slope culture medium. Cultivate for 3 to 4 days at 25~28℃. When white hyphae grow around the small tissues, select pure white hyphae and transplant them to a new slant medium. Continue to cultivate for about 5 days to obtain Ganoderma lucidum mother Species, and then expand into the original species and cultivated species.

[0017] 2) Preparation of culture medium: the following parts by weight of raw materials: 30 parts of sawdust, 10 parts of corn cob, 5 parts of rice bran, 1 part of Grifola frondosa, and 2 parts of burdock; the above raw materials are mixed and crushed, and water is added to a moisture content of 60 %, r...

Embodiment 2

[0022] 1) Selection of strains: use the tissue separation method to obtain the pure mother seed, and use 75v% alcohol to disinfect a part of the wild or artificially cultivated fresh Ganoderma lucidum fruit body, and then cut it into 3 by aseptic operation ~5mm small pieces, take about 5 pieces and place them on the plate culture medium, or take one piece and place them on the test tube slope culture medium. Cultivate for 3 to 4 days at 25~28℃. When white hyphae grow around the small tissues, select pure white hyphae and transplant them to a new slant medium. Continue to cultivate for about 5 days to obtain Ganoderma lucidum mother Species, and then expand into the original species and cultivated species.

[0023] 2) The following parts by weight of raw materials: 35 parts of sawdust, 20 parts of corn cob, 10 parts of rice bran, 3 parts of Grifola frondosa, and 4 parts of burdock; mix and pulverize the above raw materials, add water to 65% of water content. Bagging. Each bag of...

Embodiment 3

[0028] 1) Selection of strains: use the tissue separation method to obtain the pure mother seed, and use 75v% alcohol to disinfect a part of the wild or artificially cultivated fresh sweet mushroom fruit bodies, and then cut them into aseptic methods For small pieces of 3 to 5 mm, take 5 pieces and place them on the plate culture medium, or take one piece and place them on the test tube slope culture medium. Cultivate for 3 to 4 days at 25~28℃. When white hyphae grow around the small tissues, select pure white hyphae and transplant them to a new slant medium. Continue to cultivate for about 5 days to obtain Ganoderma lucidum mother Species, and then expand into the original species and cultivated species.

[0029] 2) The following parts by weight of raw materials: 32 parts of sawdust, 15 parts of corn cob, 8 parts of rice bran, 2 parts of Grifola frondosa and 3 parts of burdock; mix and crush the above raw materials, add water to 62% water, and then bag . Each bag of dry materi...

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