Brucella melitensis immune labeled recombinant strain and application thereof in preparation of vaccine

Abstract

The invention discloses a brucella melitensis immune labeled recombinant strain and the application thereof in the preparation of vaccine. The brucella melitensis immune labeled recombinant strain is obtained by inactivating phosphoglucomutase in brucella melitensis. Compared with the original strain, the strain obtained in the invention has little strain toxicity and can be taken as the vaccine for immunization of animals without inactivation. When the strain is used for the immunization of a mouse, immune response state which is different from standard strain and the existing vaccine strain can be generated in the body of the mouse, and the diseased animal can be distinguished from the group of immunized animals by immunology detection of animal serum. The invention is beneficial to the reformation of brucellosis vaccine, the research and the preparation of differential diagnosis reagent and the development of the brucella gene-deleted marking vaccine and the differential diagnosis reagent matched with the vaccine, and has very important significance on promoting the elimination of brucellosis of the animals all over the world.

Description

technical field [0001] The invention relates to a Brucella melis immunomarker recombination strain and its application in vaccine preparation. Background technique [0002] Brucellosis is a zoonotic infectious disease, second only to foot-and-mouth disease among large animal infectious diseases, and has important political and economic influences. The epidemic situation in countries around the world is very serious. For a long time, the global strategy for the prevention and control of brucellosis has been mainly quarantine-culling (in developed countries) or immunization (in developing countries). The former uses quarantine and culling of sick cattle, sheep, pigs and other animals as a measure, which costs a lot of money , Difficult to operate; the latter can prevent the spread of the disease in the animal population through immunization and auxiliary culling measures, but it is difficult to distinguish the immune animals from the clinically diseased animals, which makes th...

AI Technical Summary

Problems solved by technology

For a long time, the global strategy for the prevention and control of brucellosis has been mainly quarantine-culling (in developed countries) or immunization (in developing countries). The former uses quarantine and culling of sick cattle, sheep, pigs and other animals as a measure, which costs a lot of money , Difficult to operate; the latter can prevent the spread of the disease in the animal population through immunization and auxiliary culling measures, but it is difficult to distinguish the immune animals from the clinically diseased animals, which makes the diseased animals exist in the natural population for a long time, posing a serious threat Human and fauna health, hindering eradication and decontamination of animal brucellosis

Images