Albumin hydrogel as well as preparation method and application thereof

An albumin and hydrogel technology, applied in the field of its preparation, albumin hydrogel, can solve the problems of long gelation time, limited application, high gelation concentration, etc., to reduce the number of administrations, stable and sustained release , the effect of low gel concentration

Active Publication Date: 2020-09-04
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these hydrogels reported so far have certain disadvantages in preparation, such as long gelation time, high gelation concentration, and the need for potentially toxic small molecules as cross-linking agents, which greatly limit their application in cell culture and tissue. engineering application

Method used

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  • Albumin hydrogel as well as preparation method and application thereof
  • Albumin hydrogel as well as preparation method and application thereof
  • Albumin hydrogel as well as preparation method and application thereof

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preparation example Construction

[0047]The present invention also provides a method for preparing the above-mentioned albumin hydrogel, comprising the following steps:

[0048] The albumin solution is mixed with the cross-linking agent solution to obtain albumin hydrogel.

[0049] Specifically, albumin is dissolved in a solvent to obtain an albumin solution. The solvent is selected from water, physiological saline, buffer solution, tissue culture fluid or body fluid, preferably buffer solution. The mass volume concentration of albumin in the albumin solution is 4%-20%, preferably 5%-15%.

[0050] The crosslinking agent is dissolved in a solvent to obtain a crosslinking agent solution. The solvent is selected from water, physiological saline, buffer solution, tissue culture fluid or body fluid, preferably buffer solution. The mass volume concentration of the cross-linking agent in the cross-linking agent solution is 4%-20%, preferably 5%-15%.

[0051] The albumin solution is then mixed with a crosslinker s...

Embodiment 1

[0059] Add 300 mg of bovine serum albumin into deionized water, stir to dissolve, and adjust the pH to 7.0 with 0.2 mol / L sodium hydroxide solution. Add 15 mg of paclitaxel into absolute ethanol, stir to dissolve. The bovine serum albumin solution was stirred at a speed of 500r / min, and the paclitaxel ethanol solution was added dropwise to the bovine serum albumin at a speed of 0.5mL / min, and stirred at room temperature for 24h. After the reaction, the reaction solution was added into a dialysis bag, dialyzed with deionized water for three days, and then freeze-dried to obtain bovine serum albumin / paclitaxel nanoparticles.

[0060] The obtained nanoparticles were analyzed by high performance liquid chromatography, and the results showed that paclitaxel was successfully encapsulated in bovine serum albumin, and the drug loading was 2.0%.

Embodiment 2

[0062] Add 300 mg of bovine serum albumin into deionized water, stir to dissolve, and adjust the pH to 7.7 with 0.2 mol / L sodium hydroxide solution. Add 15 mg of paclitaxel into absolute ethanol, stir to dissolve. The bovine serum albumin solution was stirred at a speed of 500r / min, and the paclitaxel ethanol solution was added dropwise to the bovine serum albumin at a speed of 0.5mL / min, and stirred at room temperature for 24h. After the reaction, the reaction solution was added into a dialysis bag, dialyzed with deionized water for three days, and then freeze-dried to obtain bovine serum albumin / paclitaxel nanoparticles.

[0063] The obtained nanoparticles were analyzed by high performance liquid chromatography, and the results showed that paclitaxel was successfully encapsulated in bovine serum albumin, and the drug loading was 3.9%.

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Abstract

The invention provides albumin hydrogel. The albumin hydrogel is prepared by mixing albumin, a cross-linking agent and a solvent, the cross-linking agent comprises a repeating unit having a structureof formula (I) and an end group having a structure of formula (II). The invention develops the albumin hydrogel which is rapid in crosslinking, low in gelatinization concentrating and free of biotoxicity, and the albumin hydrogel is applied to the fields of cell culture, tissue engineering and drug delivery. Besides, as a good paclitaxel topical treatment drug carrier, the albumin hydrogel overcomes the defect of poor paclitaxel solubility, polyoxyethylene castor oil with biotoxicity is not introduced, and compared with a systemic preparation, the albumin hydrogel has the advantages that the administration frequency is effectively reduced, the drug concentration at a tumor is increased, and the systemic toxicity is reduced. Nanoparticles wrapping paclitaxel are used as gel forming components of the gel, so that uniform distribution of the paclitaxel in the gel can be realized, and stable and continuous release is realized.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to an albumin hydrogel, its preparation method and application. Background technique [0002] Chemically cross-linked hydrogels are materials that are cross-linked by covalent bonds, have a three-dimensional (3D) network structure and contain a large amount of water or biological fluids, and usually have good stability, durability, and mechanical properties. According to the source of the components, hydrogels can be divided into two categories: natural polymers and synthetic polymers. Natural polymers mainly include polysaccharides, such as chitosan, hyaluronic acid and proteins, such as collagen, gelatin, albumin, etc.; synthetic polymers mainly include polymethacrylic acid derivatives, polyesters, polyethers and polyethers. Amino acid materials, etc. Among such a wide variety of hydrogels, protein-based hydrogels constructed using natural proteins as basic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08J9/28A61K9/06A61K31/337A61K47/42A61L27/22A61L27/52A61L27/54A61P35/00C12N5/077C08L89/00C08L71/02
CPCA61K9/06A61K31/337A61K47/42A61L27/227A61L27/52A61L27/54A61L2300/216A61L2300/416A61P35/00C08J3/075C08J3/246C08J9/28C08J2201/0484C08J2371/02C08J2389/00C08J2471/02C08J2489/00C12N5/0656C12N2533/50C08L89/00
Inventor 贺超良王天然张震李杲陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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