Electrochemical screening and early diagnosing instrument for malignant tumor

A malignant tumor, electrochemical technology, applied in the field of immunoassay, rapid electrochemical screening and early diagnosis instrument, can solve the problem that the concentration value cannot be given intuitively, the image results of chip analysis cannot be provided, and the specificity of tumor-related antigens cannot be broken through. and other problems to achieve the effect of improving detection speed and efficiency, reducing sample consumption and shortening incubation time

Inactive Publication Date: 2006-11-22
NANJING UNIV
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  • Claims
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Problems solved by technology

These methods have certain practicability, but because they cannot give the concentration value intuitively, nor can they provide image results that are generally practical in chip analysis, and they have not broken through the specificity of tumor-associated antigens, so detection instruments have been developed and widely used.

Method used

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  • Electrochemical screening and early diagnosing instrument for malignant tumor
  • Electrochemical screening and early diagnosing instrument for malignant tumor
  • Electrochemical screening and early diagnosing instrument for malignant tumor

Examples

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Embodiment Construction

[0029] 1. Preparation of eight-channel immunoassay chip

[0030]Utilize screen printing technology to print a substrate integrating Ag / AgCl reference electrode, carbon counter electrode and multiple (8) carbon working electrodes on PVC film. The electrode is made by printing silver conductive layer on PVC film first. , then print Ag / AgCl paste on the position of the reference electrode to form the Ag / AgCl reference electrode (b), and print the carbon paste on the position of the working electrode and the carbon counter electrode to form 8 carbon working electrodes M1, M2, M3, M4, M5, M6, M7, M8 and carbon counter electrode (c), and finally printed with green oil insulating film (d). The green oil insulating film (d) can not only protect the silver conductive layer (a), but also control the volume of the reaction.

[0031] 2. Preparation of Antigen Molecule Functionalized Membrane

[0032] Chitosan powder is ultrasonically dissolved in 1% acetic acid solution to prepare 1% ch...

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Abstract

The disclosed electrochemical screening and early diagnostic device for malignant tumor comprises: an immune detection chip (1) connected to a time-resolution multichannel potentiostat (2) that connects to a data process and display system (3) by an interface. Wherein, the chip (1) comprises eight working electrodes fixed different antigen films and Ag wire (a), Ag/AgCl reference electrode (b), a carbon-pair electrode (c), and an insulation film (d). This invention needs low cost, has intelligence and fit to fast detection.

Description

1. Technical field [0001] The invention relates to a medical diagnostic instrument, in particular to an immunoassay, fast electrochemical screening and early diagnosis instrument for tumor markers. 2. Background technology [0002] Immunoassay is a highly sensitive and selective method for detecting antibodies, antigens or related substances by using the highly specific reaction between antigen / antibody, which provides a powerful means for clinical immunoassay of serum tumor markers . Usually, the clinical immunoassay methods for serum tumor markers mostly use the "double antibody sandwich" method, such as radioimmunoassay, enzyme labeling, time-resolved fluorescence, chemiluminescence and electrochemiluminescence. Due to the radioactive hazards of radioimmunoassay and the limitations of reagent life, non-radioactive-labeled immunoassay methods have been developed one after another. These methods have greatly promoted the innovation of clinical immunoassay projects and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N27/416
CPCG01N33/5438G01N27/30G01N33/57484
Inventor 鞠熀先
Owner NANJING UNIV
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