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Rapid determination method for organophosphorus pesticide residue in vegetable and fruit

An organophosphorus pesticide, rapid determination technology, applied in the preparation of test samples, color/spectral property measurement, etc. High speed, simple equipment and low cost of measurement

Inactive Publication Date: 2004-11-17
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the sensitivity of this method is generally not high, and the measurement results are closely related to the source of the enzyme used, the color reaction, the reaction time, and the reaction temperature. It is easy to be interfered and cause missed and false detections
The detection limit of this method is very different due to the different types of pesticides, and the correlation between the determination results and the determination results of gas chromatography is poor, and often cannot truly reflect the pesticide residues in the sample.

Method used

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  • Rapid determination method for organophosphorus pesticide residue in vegetable and fruit
  • Rapid determination method for organophosphorus pesticide residue in vegetable and fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 10g green vegetables, chopped, put into a centrifuge tube; add 10ml of ethyl acetate and 3g of anhydrous sodium sulfate to the centrifuge tube, place the centrifuge tube in an ultrasonic oscillator for 2min, and then use a micropipette to extract all the extracts Transfer to another centrifuge tube. Add 2g of anhydrous sodium sulfate and 0.025g of activated carbon to the centrifuge tube containing the extract, shake for 1min, and after standing for 1min, draw 5ml of the upper layer solution with a micropipette, and insert the tip of the micropipette directly into the syringe type Filter, push out the solution, and filter to remove activated carbon suspended in the solution. The filtrate was collected in a digestion tube. Place the digestion test tube containing the filtrate in a water bath at a temperature of 65°C, and use compressed air to completely blow out the ethyl acetate in the test tube.

[0021] Add 1ml of potassium persulfate solution with a concentration of...

Embodiment 2

[0023] Peeled oranges are chopped, put 5g into a centrifuge tube, add 10ml of ethyl acetate and 10g of anhydrous sodium sulfate into the centrifuge tube, place the centrifuge tube in an ultrasonic oscillator for extraction for 2min, and then use a micropipette to All extracts were transferred to another centrifuge tube. Add 2g of anhydrous sodium sulfate and 0.05g of activated carbon to the centrifuge tube containing the extract, shake for 1min, and after standing for 1min, draw 5ml of the upper layer solution with a micropipette, and insert the tip of the micropipette directly into the syringe type Filter, push out the solution, and filter to remove activated carbon suspended in the solution. The filtrate was collected in a digestion tube.

[0024] Place the digestion test tube containing the filtrate in a water bath at a temperature of 65°C, and use compressed air to completely blow out the ethyl acetate in the test tube. Add 1ml of potassium persulfate solution with a con...

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Abstract

The invention refers to a kind of measuring method for organic phosphorus agricultural chemicals residues in garden stuff. The invention uses organic solvent to extract the organic phosphorus agricultural chemicals in fruits and vegetables, uses absorbing agent to eliminate the phosphorus extraction material of inorganic phosphorus agricultural chemicals in the extraction liquid, in odder to eliminate the disturbance. The invention uses air or nitrogen to blow off the solvent in the extraction liquid, adds in potassium sulfate solvent in the residual, decomposes, converts the organic phosphorus agriculture chemicals into orthophosphate, finally, uses molybdenum antimony splitting resisting luminosity method to measure the orthophosphate density in the decomposed liquid, thus works out the whole residue quantity of organic phosphorus agricultural chemicals in the fruits and vegetables. The speed is fast, it is accurate, stable, and reliable.

Description

(1) Technical field [0001] The invention relates to a method for determining the residual amount of organophosphorus pesticides in fruits and vegetables. (2) Background technology [0002] At present, the determination methods of organophosphorus pesticide residues in fruits and vegetables mainly include gas chromatography and enzyme inhibition. The results of gas chromatography are accurate, but expensive instruments and skilled operators are required, and the operation is cumbersome, time-consuming, and consumes a large amount of organic solvents. Including sample pretreatment, it takes about 2 hours and nearly 100 ml of solvent to analyze a single sample. Gas chromatography is generally used as a standard method or referee method, and it is not suitable for rapid determination of organophosphorus pesticides in a large number of samples. The advantage of the enzyme inhibition method is that it is low in cost and less time consuming. The determination of a single sample c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N21/25
Inventor 袁东星李权龙
Owner XIAMEN UNIV
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