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Fluorescent capillary reaction apparatus and fluorescent capillary analytical method

A technology of reaction device and fluorescence analyzer, applied in fluorescence/phosphorescence, material analysis by optical means, measurement device, etc., can solve the problems of immobilized enzyme fluorescence analysis, etc., and achieve the reduction and increase of fluorescence self-quenching phenomenon Light-receiving area, effect of reducing dosage

Inactive Publication Date: 2006-02-22
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] d). Using the test solution tank, only liquid enzyme fluorescence analysis can be performed, and immobilized enzyme fluorescence analysis cannot be realized

Method used

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  • Fluorescent capillary reaction apparatus and fluorescent capillary analytical method
  • Fluorescent capillary reaction apparatus and fluorescent capillary analytical method
  • Fluorescent capillary reaction apparatus and fluorescent capillary analytical method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Utilize the present invention to riboflavin (VB 2 ) standard solution (S) concentration was determined. Measuring conditions: excitation light wavelength 500nm, emission light wavelength 550nm, room temperature 20°C, sample volume 10 μL. When measuring, place the fixed seat (5) of the fluorescent capillary reaction device in the light path of the fluorescence analyzer; use the capillary (7) to inhale VB with concentrations of 0.10, 0.50, 1.00, 1.50, 2.00, and 2.50 μg / L respectively. 2 After the standard solution, insert the capillary (7) into the insertion hole (6) on the top of the holder (5) respectively, and measure its fluorescence intensity, the results are as follows: Figure 4 shown. VB 2 There is a good linear correlation between concentration and fluorescence intensity in the concentration range of 0.10-2.50μg / L.

Embodiment 2

[0063] In this embodiment, the concentration of reduced coenzyme I (NADH) is determined by using the present invention. Measuring conditions: the wavelength of excitation light is 352nm, the wavelength of emission light is 456nm, the room temperature is 20°C, and the sample volume is 10 μL. When measuring, place the fixed seat (5) of the fluorescent capillary reaction device in the light path of the fluorescence analyzer; use the capillary (7) to inhale NADH standard solutions with concentrations of 50, 100, 150, 200, 250, and 350 μmol / L respectively , insert the capillary (7) into the insertion hole (6) on the top of the holder (5) respectively, and measure the fluorescence intensity, the results are as follows Figure 5 shown. There is a good linear correlation between NADH concentration and fluorescence intensity in the concentration range of 50-350μmol / L. With oxidized coenzyme I, coenzyme II (NAD + or NADP + ) as a coenzyme dehydrogenase reaction, the final product is...

Embodiment 3

[0065] This example is based on the determination of ethanol concentration by the immobilized enzyme FCA method. Measuring conditions: the excitation light wavelength is 352nm, the emission light wavelength is 456nm, the room temperature is 20°C, and the amount of the liquid to be tested is 10 μL. Before the measurement, the enzyme is fixed on the inner wall of the capillary (7) to make an ethanol capillary bioreactor. During the measurement, the fixed seat (5) of the fluorescent capillary reaction device is placed in the light path of the fluorescence analyzer; the ethanol standard solution and the reaction concentration of 2, 4, 8, 10, 15 g / L are inhaled respectively with the ethanol capillary bioreactor. After the mixed solution of the reagent is inserted into the insertion holes (6) of the fixing seat (5) respectively, the fluorescence intensity is measured, and the results are as follows: Image 6 shown. There is a good linear correlation between ethanol concentration a...

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Abstract

This invention discloses one fluorescent capillary reaction device and it analysis method, wherein the device comprises fixing socket, capillary insert hole, laser emission window, fluorescent window and capillary. The method comprises the following steps: executing FCA method; putting the socket into fluorescent light path; inserting capillary into liquid to be tested into socket top; the laser passing socket emission window on capillary to generate fluorescent light from fixed socket emission window through single color device at tester.

Description

technical field [0001] The invention relates to a fluorescent capillary reaction device and a fluorescent capillary analysis (FCA), belonging to the technical field of biochemical analysis and quantification. The invention is applicable to the fluorescence analysis of various trace components in trace samples of medicine, medicine, food, sanitation, industry, agriculture and forestry, environment and the like. Background technique [0002] When many substances absorb ultraviolet light and radiation in the visible spectral region, their electrons transition from the ground state to the excited state. When the electrons in the excited state return to the ground state, part of the energy is released in the form of thermal radiation, while the other part is in the form of light. This light is called fluorescence. The method of chemical analysis of unknown samples using fluorescence is called fluorescence analysis. [0003] The fluorescence analy...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
Inventor 李永生高秀峰
Owner SICHUAN UNIV
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