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Method for detecting 1,5-dehydration glucitol in blood and kit

An anhydroglucitol and kit technology, which are applied in the fields of biochemical technology and biochemical testing, can solve the problems that specificity needs to be further improved, the source of raw materials is difficult, the source is difficult, etc., so as to improve specificity and accuracy, high sensitivity, and easy operation. simple effect

Active Publication Date: 2011-09-07
BEIJING STRONG BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main disadvantage is that the source of raw materials is difficult, and the prices of diaphorase (DI), 1,5-anhydroglucitol dehydrogenase (1,5-AG-HK) and pyranose oxidase (PROD) (oxidase method) are relatively low. High and difficult to source
At present, most of the reagents on the market are dry powder reagents (existing reagents have poor stability), and the detection is easily interfered by endogenous substances such as glucose, bilirubin, ascorbic acid, blood lipids, etc., and the specificity needs to be further improved

Method used

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  • Method for detecting 1,5-dehydration glucitol in blood and kit
  • Method for detecting 1,5-dehydration glucitol in blood and kit
  • Method for detecting 1,5-dehydration glucitol in blood and kit

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Embodiment Construction

[0056] 1. Preparation of detection reagents

[0057] Pick:

[0058] Glucose oxidase (GOD) 15KU

[0059] Catalase (CAT) 2KU

[0060] Ascorbate Oxidase (ASO) 10KU

[0061] Cetyltrimethylammonium bromide 1g

[0062] Potassium ferrocyanide 100umol

[0063] Phosphate buffer saline (PBS) 100mmol

[0064] Polyethylene glycol 0.5g

[0065] Sodium Benzoate 0.5g

[0066] Prepare 1 liter of solution to make reagent I;

[0067] Pick:

[0068] Pyranose oxidase (PROD) 200KU

[0069] 4-Aminoantipyrine (4-AAP) 5mmol

[0070] Horseradish Peroxidase (HRP) 10KU

[0071] 3-Hydroxy-2,4,6-trihydroxybenzoic acid (HTIB) 5mmol

[0072] Phosphate buffer saline (PBS) 400mmol

[0073] Polyethylene glycol 0.5g

[0074] Sodium azide 0.5g

[0075] Prepared into 1L solution to make reagent II.

[0076] 2. Preparation of 1,5-anhydroglucitol calibrator: Weigh the required amount of pure product (purchased by Sigma) and dissolve it in purified water, add 0.1% sodium azide as preservative, and se...

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Abstract

The invention relates to a method for detecting 1,5-dehydration glucitol in blood and a kit, and provides a new method for detecting the content of the 1,5-dehydration glucitol in the blood, which comprises the following steps of: catalyzing the 1,5-dehydration glucitol by using pyranose oxidase to generate 1,5-dehydration fructose and H2O2; generating quinine compounds by using 4-aminoantipyrine(4-AAP), 3-hydroxy-2,4,6-trihydroxybenzoic acid (HTIB) and H2O2 under the catalytic action of horse radish peroxidase; and determining the level of the 1,5-dehydration glucitol in the blood by colorimetric analysis. The invention also provides the kit for the method. The method and the kit are easy and convenient to operate safely, long in stable time and high in interference resistance, specificity and sensitivity, the interference of multiple glucoses in blood samples can be eliminated quickly, and the reliable foundation can be provided for the diagnosis and treatment of diabetes.

Description

technical field [0001] The invention relates to biochemical technology and biochemical testing technology, in particular to a detection method and a kit for quantitatively measuring the content of 1,5-anhydroglucitol (1,5-AG) in blood in vitro. Background technique [0002] Diabetes is a common and frequently-occurring disease in clinical practice. It is caused by insufficient secretion of insulin. 1,5-sorbitol (1,5-AG) in the blood is in the same state as glucose (GLU), glycosylated hemoglobin (HbAlc), and fructosamine (FMN). significant negative correlation. According to data, none of the patients with plasma glucose (GLU)>8.3mmol / L was accompanied by a high level of plasma 1,5-anhydroglucitol (1,5-AG). [0003] 1,5-anhydroglucitol (1,5-AG) can be used as a sensitive and specific index for early diagnosis of diabetes patients, and can also be used as an evaluation index for diabetes treatment and prognosis observation. The current diabetes diagnostic indicators have o...

Claims

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Application Information

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IPC IPC(8): G01N21/78C12Q1/30C12Q1/28C12Q1/26
Inventor 杜爱铭刘瑶刘希蔡华雅
Owner BEIJING STRONG BIOTECH INC
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