Preparation method of electrochemistry immunosensor for determining alpha fetoprotein

A technology of immune sensor and alpha-fetoprotein, which is applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high requirements for radioactive material equipment, unstable enzymes, and great harm, and achieve excellent accuracy, rapid detection, and Good biocompatibility effect

Active Publication Date: 2012-06-20
ANHUI DEXINJIA BIOPHARM
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Problems solved by technology

These methods are sensitive and reliable, but the enzymes are not stable, the ...

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Embodiment 1

[0028] Step 1. Amino-modified nano-mesoporous SiO 2 and H 4 AuCl 4 Mix according to the ratio of mass 1.2:1, at room temperature, stir gently with a magnetic stirrer, then add hydrochloric acid to adjust the acidity, then stir again, add 40mmol·L -1 Sodium borohydride, until its color becomes black (purple, black), centrifugal, room temperature vacuum drying, can obtain MSN-Au nanocomposite material;

[0029] Step 2. Add 1.0mL of 1.0mg·mL -1 MSN-Au nanocomposite solution and 1.0mL 1.0mg·mL -1 HRP solution and 1.0mL 1.0mg·mL -1 Ab 2 The solution was mixed, lightly shaken and adsorbed for 24h, centrifuged, and the obtained substance was redispersed in 1.0mL 0.05mol·L -1 In the PBS buffer solution of pH=7.50 for subsequent use;

[0030] Step 3. Polish the glassy carbon electrode with a diameter of 5mm with 1.0, 0.3 and 0.05μm aluminum oxide polishing powder in turn, rinse it with secondary water, and then place the electrode in 0.05mol·L -1 In potassium ferricyanide solut...

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Abstract

The invention relates to a preparation method of an electrochemistry immunosensor for detecting alpha fetoprotein, in particular to a gold-composite nanometer mesoporous SiO2 composite-based preparation method of the electrochemistry immunosensor for determining the alpha fetoprotein. The immunosensor is employed to provide a method for determining the alpha fetoprotein. The preparation method ischaracterized by comprising the steps of: firstly synthetizing a nanometer mesoporous SiO2-nano-gold composite, fixing horseradish peroxidase and a secondary antibody on the composite to form a complex; fixing a primary antibody by using a thionine-graphene complex, thereby preparing the electrochemistry immunosensor for determining the alpha fetoprotein, wherein the immunosensor is successfully used for the determination of the alpha fetoprotein. The electrochemistry immunosensor disclosed by the invention has the advantages of high accuracy, high sensitivity, stability and repeatability, rapidness and convenience for immunoassay determination, can be used for clinical analysis and provides important basis for early diagnosis of cancers.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical immunosensor for measuring alpha-fetoprotein, especially based on gold hybrid nano-mesoporous SiO 2 Preparation of composite electrochemical immunosensor for the determination of alpha-fetoprotein. Background technique [0002] In recent years, research on nanomaterials in various probe technologies at the single-molecule level, nano-integrated array devices, and electrode surface modification has made extensive progress. Among them, the introduction of nanomaterials into the electrode surface can effectively increase the specific surface area of ​​the electrode, accelerate the transfer of electrons, enhance the conductivity of the electrode, increase the immobilization of biomacromolecules such as enzymes, enhance the sensitivity and stability of the sensor, and improve the response of the sensor. speed etc. Nano-mesoporous SiO 2 The material (MSN) has the advantages of large speci...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N27/26
Inventor 魏琴李贺韩颜颜李玉阳杜斌吴丹马洪敏庞雪辉罗川南范大伟李燕辛晓东赵燕芳蔡燕燕杨健崔振涛毛珂霞
Owner ANHUI DEXINJIA BIOPHARM
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