High stable enhanced chemiluminescence substrate

A technology that enhances chemical and luminescent substrates. It is applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, and biological testing. It can solve problems such as deviation of detection results and unstable optical signals.

Inactive Publication Date: 2005-10-26
段虎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the existing technology, first, H2O2 is gradually destroyed with the prolongation of storage time, and second, the generated optical signal is unstable, which causes deviations in the detection results

Method used

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  • High stable enhanced chemiluminescence substrate

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Experimental program
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Embodiment Construction

[0018] Preparation of substrate solution: 0.5mol / L boric acid buffer solution with pH8.6 to prepare liquid A and liquid B respectively: liquid A contains luminol 3.0mg / ml, enhancer p-iodophenol 0.25mg / ml, synergistic enhancer tetraphenyl Boron sodium 0.5mg / ml; B solution contains carbamide peroxide 0.4g / ml, H 2 o 2 0.1g / ml.

[0019] High storage stability. Liquid A and liquid B are stored separately. It is valid for 2 years when stored at 2-8°C.

[0020] High reaction stability. Add equal volumes of mixed liquid A and liquid B into the HRP-labeled immunoreaction sample tube (hole), and the dose-response curve of the same series of calibrator determined in 0-4 hours is stable (with attached picture); the same sample in 0-4 hours The coefficient of variation of the measured value is less than 5%, and the corresponding coefficient of variation (CV%) of the luminous value is less than 15%.

[0021] Analyte 1 (5.3ng / ml)

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Abstract

The invention relates to the high-stable increased chemical illuminant base material, which is composed of the solutions A and B. the solution A contains luminal, reinforcing agent phenol and coordinate reinforcing agent; the solution B contains urea peroxide and H2O2. The illuminant signal of the invention is stable and is suitable for the automatic or hand analysis, and the testing results are precise. The 2-8 Deg. C. storing stability can last for two years.

Description

technical field [0001] The invention relates to chemiluminescence analysis technology, which is an ultra-trace analysis method, and belongs to the technical field of "optical means test and analysis" of international patent classification GO1N21 / 17. Background technique [0002] Chemiluminescence analysis technology is one of the modern micro-analysis methods. Because of its high sensitivity, simplicity and speed, good linearity, long validity period, safety and harmlessness (no radioactive and teratogenic pollution), it has become an important technology to replace radioimmunoassay and enzyme-linked . The chemical analysis method labeled with horseradish peroxidase is to add HRP substrate H2O2 after the completion of the immune reaction. Under alkaline conditions, HRP catalyzes H2O2 to make the luminescent substrate luminol undergo a chemical reaction and emit light. However, in the existing technology, first, H2O2 is gradually destroyed as the storage time prolongs, and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 段虎
Owner 段虎
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