Enzyme test peper for detecting hydrogen peroxide concentration

A technology of horseradish peroxidase and test paper, which is applied in the field of enzymatic analysis in the field of biotechnology, can solve the problems of cumbersome operation and unfavorable wide use, and achieve the effect of being easy to carry and suitable for mobile operations

Inactive Publication Date: 2006-08-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, another serious problem of hydrogen peroxide residues in food is that many criminals in the market abuse hydrogen peroxide to bleach watery foods such as beef louver, chicken feet and bamboo shoots, and even to bleach and eliminate the peculiar smell of spoiled seafood. Wait
However, these methods are cumbersome to operate and require special laboratory equipment such as spectrophotometers, which is not conducive to the widespread use in the food industry and other monitoring fields.

Method used

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  • Enzyme test peper for detecting hydrogen peroxide concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Preparation of Enzyme Test Paper for Determination of Hydrogen Peroxide Concentration

[0016] Take thick qualitative filter paper and cut it into 1cm×3cm size, soak it in 1% carboxymethyl cellulose aqueous solution for about 60 minutes, after fully imbibition, dry it, and then soak the filter paper strip in 20ml containing color reagent And in the mixed solution of horseradish peroxidase, wherein the composition of mixed solution is: phenol 0.025M, 4-aminoantipyridine 0.0075M, horseradish catalase 0.025g / L (W / V), ampicillin Penicillin 100mg / L (W / V). After shaking at 4°C for 60 minutes, take out the filter paper, dry it at low temperature, and finally store it in a sealed plastic bag at low temperature and avoid light. That is, the enzyme test paper that can be used for the determination of hydrogen peroxide concentration is prepared.

Embodiment 2

[0018] Preparation of Enzyme Test Paper for Determination of Hydrogen Peroxide Concentration

[0019] Cut the carboxymethyl cellulose membrane into a size of 1cm×3cm (because the carrier itself is carboxymethyl cellulose, which already has negatively charged anionic groups, so it is not necessary to soak it in carboxymethyl cellulose aqueous solution), then soak it in 20ml In the mixed solution of chromogen and horseradish peroxidase, wherein the mixed solution consists of: phenol 0.02M, 4-aminoantipyridine 0.005M, horseradish catalase 0.01g / L (W / V ), tetracycline 10mg / L (W / V). After shaking at 4°C for 60 minutes, take out the filter paper, dry it at low temperature, and finally store it in a sealed plastic bag at low temperature and avoid light. That is, the enzyme test paper that can be used for the determination of hydrogen peroxide concentration is prepared.

Embodiment 3

[0021] Preparation of Enzyme Test Paper for Determination of Hydrogen Peroxide Concentration

[0022] Cut the nitrocellulose membrane into a size of 1cm×3cm (because the carrier itself is nitrocellulose, which already has a negatively charged anion group, so it is not necessary to soak it in an aqueous carboxymethyl cellulose solution), and then soak it in 20ml of chromogen and In the mixed solution of horseradish peroxidase, wherein the mixed solution is composed of: phenol 0.10M, 4-aminoantipyridine 0.05M, horseradish catalase 0.025g / L (W / V), kana Mycin 50mg / L (W / V). After shaking at 4°C for 60 minutes, take out the filter paper, dry it at low temperature, and finally store it in a sealed plastic bag at low temperature and avoid light. That is, the enzyme test paper that can be used for the determination of hydrogen peroxide concentration is prepared.

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Abstract

This invention relates to a kind of enzyme test paper capable of testing hydrogen peroxides in a sample quickly prepared by an enzymatic chromogenic reaction, which utilizes the adsorption action with anion carriers to fix the horseradish peroxide enzymes with positive charges and a developer on a carrier at the same time, which can test the existence of hydrogen peroxides with the concentration to 0.5mumol / L in the sample and the response time of the test paper is 1 minute.

Description

technical field [0001] The invention relates to an enzyme test paper for rapidly detecting the content of hydrogen peroxide in a sample by using an enzymatic color reaction, and belongs to the field of enzymatic analysis in the field of biotechnology. Background technique [0002] Hydrogen peroxide, commonly known as hydrogen peroxide, can be decomposed into water and oxygen under conditions such as stirring and light, and is extremely oxidizing and corrosive, so it is widely used as a disinfectant and bleaching agent for food. According to my country's "Hygienic Standards for the Use of Food Additives", food-grade hydrogen peroxide is allowed to be used as a processing aid in food production, but it should be removed before it is made into a finished product, and the residue should be strictly controlled. Excessive hydrogen peroxide residues in food will cause serious harm to the human body, such as: damage the digestive tract, cause cancer, accelerate human aging, induce c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78C12Q1/00
Inventor 王炜军吕志刘娥娥方颖徐凤彩
Owner SOUTH CHINA AGRI UNIV
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