The present disclosure provides a method for extracting and purifying small rubber particle
protein from
Hevea brasiliensis latex, which comprises: adding an alkaline
buffer solution to the latex to stabilize the latex; precipitating and removing most of the proteins and all latex
solid components in the latex by a strong
anionic detergent and an
organic solvent, but the small rubber particle
protein remains in a dissolved state; centrifuging the mixture to clarify the liquid portion, and transferring the clarified liquid portion; adding
ammonium sulfate to the liquid portion and oscillating to precipitate the proteins, thereby obtaining a crude
protein extract (mainly containing small rubber particle protein); separating the crude protein extract by
ion exchange
chromatography, collecting the main
elution peak; further separating the proteins contained in the main
elution peak of
ion exchange
chromatography by gel
filtration chromatography, thereby obtaining purified small rubber particle protein. The method of the present disclosure can efficiently separate target proteins from non-target proteins in a time-saving manner.