3 results about "Anionic detergent" patented technology

This invention relates to solid particles of a linear alkylbenzene sulfonate anionic detergent surfactant. These particles contain a polycationic salt of linear alkylbenzene sulfonic acid having both magnesium and sodium cations. The weight ratio of the magnesium salt of linear alkylbenzene sulfonic acid to the sodium salt of linear alkylbenzene sulfonic acid is in the range of 0.6:1 to 9:1.

The present disclosure provides a method for extracting and purifying small rubber particle protein from Hevea brasiliensis latex, which comprises: adding an alkaline buffer solution to the latex to stabilize the latex; precipitating and removing most of the proteins and all latex solid components in the latex by a strong anionic detergent and an organic solvent, but the small rubber particle protein remains in a dissolved state; centrifuging the mixture to clarify the liquid portion, and transferring the clarified liquid portion; adding ammonium sulfate to the liquid portion and oscillating to precipitate the proteins, thereby obtaining a crude protein extract (mainly containing small rubber particle protein); separating the crude protein extract by ion exchange chromatography, collecting the main elution peak; further separating the proteins contained in the main elution peak of ion exchange chromatography by gel filtration chromatography, thereby obtaining purified small rubber particle protein. The method of the present disclosure can efficiently separate target proteins from non-target proteins in a time-saving manner.

A method for determining a level of one or more live microbes in a sample of a Cannabis plant includes (a) introducing a DNase enzyme into a sample including (i) Cannabis plant matrix derived from lysed cells of a Cannabis plant and (ii) live microbial cells including microbial genomic DNA, (b) introducing an anionic detergent into the sample, where the anionic detergent is in an amount effective to inactivate the DNase enzyme in the sample and lyse the live microbial cells in the sample, (c) introducing a known quantity of control DNA into the sample, (d) contacting the sample with a plurality of polynucleotide capture agents, (e) isolating the plurality of polynucleotide capture agents, thereby isolating polynucleotides from the microbial genomic DNA and / or control DNA in the sample, and (f) amplifying at least a portion of polynucleotides from the microbial genomic DNA isolated in step (e).