7 results about "Oligonucleotide Linker" patented technology

The present invention relates to the detection of modifications in polynucleotides by binding an affinity binding element to a nucleotide sequence. The affinity binding element may include a compound that binds to a modified nucleotide base and a nucleotide sequence complementary to a target nucleotide sequence, an oligonucleotide primer complementary to the target nucleotide sequence, or a nucleotide sequence complementary to an oligonucleotide linker. The present invention provides methods for detecting one or more modifications in a nucleotide sequence. The invention also provides affinity binding elements, and optionally, oligonucleotide primers and / or oligonucleotide probes, and methods of using the affinity binding elements in assays to detect modified bases. The methods of the invention enable the detection of modified nucleotides associated with cancer, psychiatric disorders or metabolic diseases.

Provided is a method of preparing at least one ligation product from a sample including a plurality of single-strand nucleic acid fragments, the method including the steps of: (a) ligating a first universal oligonucleotide adaptor to at least one single-strand nucleic acid fragment, wherein the first universal oligonucleotide adaptor is configured for ligating to a 3′ end of individual single-strand nucleic acid fragment; and (b) ligating a second universal oligonucleotide adaptor to the at least one single-strand nucleic acid fragment, wherein the second universal oligonucleotide adaptor is configured for ligating to a 5′ end of individual single-strand nucleic acid fragment, thereby at least one ligation product is formed. In another embodiment, provided is at least one cancer biomarker comprising human telomere sequence with two or more consecutive repeats of nucleotide sequence TTAGGG.

The invention relates to the field of sequencing, in particular to an oligonucleotide connector containing an RNA bar code and application of the oligonucleotide connector. By using the oligonucleotide linker provided by the invention, multiple biological samples can be simultaneously sequenced in one chip, and extremely-low initial amount total RNA (Ribonucleic Acid) and even single cell level DRS (Dead Respiratory Syndrome) sequencing of a single sample can also be realized; the requirement on the initial quantity of RNA samples is reduced, and a powerful tool is provided for detecting rare samples or micro / trace RNA.

In a first aspect, the application provides a marker (100, 400, 500, 600) for analyzing a biological sample. The marker (100, 400, 500, 600) comprises an affinity reagent (102), in particular the affinity reagent (102) is configured to specifically attach to a target analyte of a biological sample. The marker (100, 400, 500, 600) further comprises at least one marker moiety (104, 602, 604) and at least one linker oligonucleotide (106, 204, 404, 506a, 506b, 506c). A linker oligonucleotide (106, 204, 404, 506a, 506b, 506c) is attached to the affinity reagent (102), and at least one marker moiety (104, 602, 604) is attached to the linker oligonucleotide (106, 204, 404, 506a, 506b, 506c). In another aspect, the present application provides a method for generating a marker (100, 400, 500, 600).

The invention provides a protein linker system for conjugating oligonucleotides to the surface of a substrate. Subjecting the protein linker system to a protease releases conjugated oligonucleotides from the surface of the substrate. The inventive protein linker system avoids the use of chemical-cleavable and photo-cleavable oligonucleotide linkers, and permits the use of plastic substrates which maintain their shape and size during changes in salt and detergent concentrations in the substrate environment.

Disclosed herein are oligonucleotide conjugates. An example conjugate includes an oligonucleotide; a binding ligand; an endosomal escape portion comprising an endosomal escape moiety; and a linker attached to the endosomal escape portion, wherein: the oligonucleotide is attached to the endosomal escape portion, the linker attaching the endosomal escape portion to the binding ligand; or the binding ligand is attached to the oligonucleotide, the linker attaching the endosomal escape portion to the oligonucleotide. Also disclosed are compositions including the conjugate and methods of using the conjugate.

Building DNA strands at a high rate that are suitable for data storage. Methods include using DNAzyme and utilizing libraries of pre-prepared oligos. A system for the DNA strand synthesis includes: a DNA symbol library comprising a number of single strand oligo symbols; a DNA linker library comprising a first set of single strand oligo linkers and a second set of single strand oligo linkers; and a DNAzyme library comprising a number of DNAzymes. An S1 end of a first DNAzyme is adapted to join the S1 end of a symbol and an S2 end of the first DNAzyme is adapted to join an S2 end of a first linker, and an S1 end of a second DNAzyme is adapted to join an S1 end of a second linker and an S2 end of the second DNAzyme is adapted to join an S2 end of the symbol.