Novel quencher and reporter dye combinations

Abstract

Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

Description

technical field [0001] The present specification generally relates to FRET pairs comprising quencher and reporter dye combinations for biological applications including, for example, polymerase chain reaction (PCR), such as real-time or quantitative PCR (qPCR). Background technique [0002] Fluorescent energy transfer (FRET) in double-labeled oligonucleotide probes is widely used in the determination of gene analysis. FRET has been used to study DNA hybridization and amplification, protein folding kinetics, proteolytic degradation, and interactions between other biomolecules. Nucleic acid detection / amplification methods such as the use of dual-labeled probes in real-time polymerase chain reaction to detect and / or quantify target nucleic acids such as specific gene sequences or expressed messenger RNA sequences. Fluorescent probes for use in this method are typically labeled with reporter and quencher moieties. In this case, when the two dyes are physically separated by hyb...

AI Technical Summary

Problems solved by technology

Until now, most multiplex PCR instruments could only utilize four unique dye-quencher pairs

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