Talaromyces emersonii enzyme systems

A technology of Talaromyces and strains, applied in the directions of enzymes, hydrolases, glycosylases, etc., can solve the problems of not considering the display of multiple activities, excessive consumption of time and cost, waste of processes, etc., and achieve enhanced storage and transportation. Potential, time and cost savings, effect of short reaction times

Inactive Publication Date: 2009-06-17
THE NAT UNIV OF IRELAND GALWAY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are very time-consuming and wasteful
[0007] Furthermore, these methods do not take into account the fact that some enzymes may exhibit multiple activities or may have altered efficiencies when used in combination with another enzyme
These methods can therefore lead to an overproduction of unwanted enzymes and thus also to excessive consumption and time costs

Method used

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  • Talaromyces emersonii enzyme systems
  • Talaromyces emersonii enzyme systems
  • Talaromyces emersonii enzyme systems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1T.emersonii IMI393751, separation

[0077] Freshly harvested (approximately 200 g) clean grass (turf) cuttings and other mixed plant biomass were placed in closed containers to simulate a composting environment, and incubated at 65°C for approximately 2 hours in a constant temperature climate chamber (combined pasteurization and equilibration of substrates). Keep the humidity / moisture level at -65-70%. Use a container to provide low pulses of humidified, filtered air at intervals through the line. After 2 hours, a spore suspension (1 x 10 in 2% sterile water) of T. emersonii (a laboratory stock derived from a 12-year-old isolate of CBS814.70) 8 spores) were used to inoculate the central area of ​​the biomass. The temperature was maintained at 65°C for 2 days and thereafter increased at 2°C intervals every 24 hours until the air temperature inside the climate chamber reached 70°C. The culture was grown for an additional 7 days before aseptically removing ...

Embodiment 2

[0085] Example 2: Enzyme system from Talaromyces emersonii for transformation of cellulosic material. The complete hydrolysis of xylose requires the synergistic action of xylanase, β-xylosidase, α-glucuronidase, α-L-arabinofuranosidase and esterase. Table 1. Gives examples of percentages of selected target substrates (including waste / residue) and inducible carbonogens used in this example. Percent induction refers to the weight of carbon source per volume of medium (g / 100ml).

[0086] Table 1: Culture substrates / inducible carbon sources for enzyme production by T. emersonii

[0087]

[0088] ^Included for comparison purposes.

[0089] Based on the results obtained, an enzyme system for the degradation of hemicellulose (xylan) or xylan-rich wood-derived products, residues or wastes was designed using enzymes purified from Talaromyces emersonii. The relative amounts of key enzymes present in the enzyme system are listed in Table 2.

[0090] Table 2: Systems used to degrad...

Embodiment 3

[0101] Example 3: A system from Talaromyces emersonii for the conversion of cereals, sugar beet pulp and other materials rich in arabinoxylan and acetylated hemicellulose, including waste.

[0102] Table 4 presents the Talaromyces emersonii IMI 393751 and two previously identified strains designed for the conversion of cereal cereals, sugar beet pulp, and other materials (including waste) rich in arabinoxylan and acetylated hemicellulose. Relative amounts of different enzyme activities in the designed enzyme systems of the mutant lines.

[0103] Table 4: Enzyme activity of systems used for conversion of arabinoxylan and acetylated hemicellulose rich materials

[0104] enzyme

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Abstract

The invention relates to strains of Talaromyces emersonii which are thermostable and encode thermostable enzymes. The enzymes retain activity at temperatures above 550C. These strains and enzymes find use in a variety of processes from waste reduction to the production of novel food ingredients and the production of bio-fuels.

Description

technical field [0001] The present invention relates to strains of Talaromyces emersonii and enzymes and enzyme systems isolatable therefrom for use in environmental and waste treatment, chemical and biochemical production and processing, biotechnology Methods, kits for detection or diagnosis, prebiotics and synbiotics, healthcare products, functional and novel foods and beverages, surfactant products, agricultural and horticultural applications. Background technique [0002] Europe is currently facing a waste disposal crisis, with 2 billion tons of waste generated every year. The majority of this waste is organic matter derived from plant material (biomass), rich in carbohydrates (sugars), and thus represents a valuable resource when broken down in its constituent sugars. Other types of waste such as fruit waste rot, thereby causing environmental hazards. Usually this waste is mixed with pig feed to extract something of value from it, although this is considered a low cos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24C12N9/42C12P7/06C12P19/02C12P19/04C12P19/12C12P5/02A23K1/165
CPCC12Y302/01006Y02E50/343C12Y302/01004C12Y302/01021C12P7/06C12N9/2445C12N9/248A23K1/1656Y02E50/17C12Y302/0115C12R1/645C12P19/04C12Y302/01091C12P5/023C12P19/12C12P19/02C12N9/2437A23K10/14Y02E50/10Y02E50/30C12R2001/645C12N1/145
Inventor M·G·图伊P·G·玛丽C·T·吉勒安C·M·科林斯F·J·瑞恩L·P·迈克劳林A·G·S·雷登A·P·玛劳尼M·N·赫尼甘A·J·奥多诺格C·S·马亨
Owner THE NAT UNIV OF IRELAND GALWAY
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