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Novel enzymes which dehydrate glycerol

a technology of glycerol and enzymes, which is applied in the direction of lyases, oxidoreductases, biochemical equipment and processes, etc., can solve the problems of high cost of traditional chemical synthesis methods

Inactive Publication Date: 2002-09-19
GENENCOR INT INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

0001] The present invention relates to improved methods and reagents for the production of 1,3-propanediol. In particular, the present invention provides novel thermophilic organisms and thermostable enzymes capable of catalyzing the fermentation of glycerol to 1,3-propane...

Problems solved by technology

However, traditional chemical synthesis methods are expensive and generate waste streams containing environmental pollutants, and are thus far from ideal.

Method used

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  • Novel enzymes which dehydrate glycerol
  • Novel enzymes which dehydrate glycerol
  • Novel enzymes which dehydrate glycerol

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Embodiment Construction

[0023] Thermophilic organisms are organisms that can survive and grow at elevated temperatures where most other organisms (e.g., mesophiles) would not be able to survive. This unusual resilience towards high temperatures results in part from these organisms' use of thermostable enzymes to catalyze the biological reactions of life. Thermostable enzymes allow thermophilic organisms to catalyze metabolic reactions at elevated temperatures. Such organisms can be isolated from ambient temperature environments, but are more likely isolated from high temperature environments including, for example, hot springs, thermal vents and laundromat effluents. The unusual thermostability of thermophilic bacteria and enzymes allows one to catalyze economically valuable bioconversions at higher temperatures than could be achieved using mesophilic organisms and enzymes. In many cases, thermophilic bacteria are used to catalyze the desired reaction in vivo in a fermentation process. Alternatively, therm...

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Abstract

The present invention relates to improved methods and reagents for the production of 1,3-propanediol. In particular, the present invention provides novel thermophilic organisms and thermostable enzymes capable of catalyzing the fermentation of glycerol to 1,3-propanediol. The present invention also relates to methods of isolating such thermophilic organisms, methods of cloning polynucleotides that encode such enzymes, polynucleotides encoding such enzymes, and methods of using such enzymes and organisms for the production of 1,3-propanediol.

Description

1 FIELD OF THE INVENTION[0001] The present invention relates to improved methods and reagents for the production of 1,3-propanediol. In particular, the present invention provides novel thermophilic organisms and thermostable enzymes capable of catalyzing the fermentation of glycerol to 1,3-propanediol. The present invention also relates to methods of isolating such thermophilic organisms, methods of cloning polynucleotides that encode such enzymes, polynucleotides encoding such enzymes, and methods of using such enzymes and organisms for the production of 1,3-propanediol.2 BACKGROUND[0002] 1,3-Propanediol is a monomer used in the production of polyester fibers and the manufacture of polyurethanes and cyclic compounds.[0003] A variety of synthetic routes to 1,3-propanediol are known. For example, 1,3-propanediol can be synthesized: (1) by the conversion of ethylene oxide over a catalyst in the presence of phosphine, water, carbon monoxide, hydrogen and an acid; (2) by the catalytic s...

Claims

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Application Information

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IPC IPC(8): C12N15/09C12N1/02C12N1/20C12N1/21C12N9/02C12N9/04C12N9/88C12P7/18C12P7/62C12Q1/68C12R1/01
CPCC12N9/0004C12N9/88C12P7/18C12P7/04
Inventor SEYFRIED, MARKUSWIEGEL, JUERGENWHITED, GREGORY
Owner GENENCOR INT INC
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