Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Protein containing serum albumin domain

a serum albumin and domain technology, applied in the direction of peptide/protein ingredients, drug compositions, antinoxious agents, etc., can solve the problems of human body, safety problems, pharmaceutical preparations using albumin derived from blood, etc., to improve the functional activity of albumin, improve the antioxidative ability, and improve the effect of albumin

Inactive Publication Date: 2007-08-07
NIPRO CORP
View PDF8 Cites 35 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007]The inventors of the present invention have established an expression system, which is capable of preparing a DNA sequence encoding each domain of serum albumin composed of domains I, II, and III, transforming a host cell using a vector containing such a DNA sequence, and producing a protein containing the serum albumin domain in the host cell. Further, the inventors of the present invention have conducted a functional analysis on each domain. As a result, the inventors of the present invention have found that a specific domain is responsible for most of specific biological activities of albumin. In addition, the inventors of the present invention have found that a protein having various enhanced functional activities of albumin can be provided by producing a protein containing a large amount of the specific domain by gene recombinant technology, and have completed the present invention. In particular, the inventors of the present invention have found out that albumin domain I has high antioxidative ability while showing low enzyme activity. Therefore, a protein containing domain I can provide an enhanced antioxidative effect.
[0008]As a result of the investigation of the binding property of three site I markers (subsite Ia, Ib, Ic), only subsite Ic ligand shows binding with domain II though the binding strength is weak. The site II drugs show a binding property with domain II in a ratio of 60 to 80% compared to the wild type albumin. However an esterase-like activity is shown to be remarkably high in the domain III but significantly less than a wild type albumin. An enolase-like activity is not shown in any domain at pH 7.2 but is shown in the domain II at pH 9.2. Further, it is interesting that an antioxidative effect of domain I is substantially similar to that of the wild type albumin. Moreover, each domain reflects a molecular weight to show a large clearance in the kidney. As domain I shows high antioxidant property and low enzymatic activity, it is expected that a protein genetically modified to contain albumin domain I will be applied to a drug delivery system. A protein containing a plurality of amino acid sequences corresponding to domain I will show enhanced antioxidant property.

Problems solved by technology

Meanwhile, pharmaceutical preparations using albumin derived from the blood may be contaminated with an unknown virus.
Thus, the use thereof for the human body has caused safety problems.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein containing serum albumin domain
  • Protein containing serum albumin domain
  • Protein containing serum albumin domain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0042]FIG. 1 shows an outline of procedures for preparing a human serum albumin domain I trimer of Example 1.

Amplification of DNA Fragment Encoding Human Serum Albumin Domain I

[0043]A plasmid prepared by incorporating a gene encoding human serum albumin into a plasmid pKF18K (hereinafter, pKF18K-HAS, available from TonenGeneral Sekiyu K.K.) (see FIG. 2) was used as a template. A sense primer of SEQ. ID. No. 1 and an anti-sense primer of SEQ ID NO:2, a sense primer of SEQ ID NO:3 and an anti-sense primer of SEQ ID NO:4, and a sense primer of SEQ. ID. NO:5 and an anti-sense primer of SEQ ID NO:6 were used as synthetic primers to carry out PCR using DNA polymerase (KOD-plus-, available from Toyobo Co., Ltd.). As reaction conditions for PCR, DNA was treated at 94° C. for 10 minutes, subjected to a series of reactions of denaturing (94° C., 1 min.), annealing (64° C., 1 min.), and extension (72° C., 1 min.) for 30 cycles, and then treated at 72° C. for 3 minutes. DNA fragments, to which ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pHaaaaaaaaaa
osmotic pressureaaaaaaaaaa
single stranded structureaaaaaaaaaa
Login to View More

Abstract

A protein produced by gene recombinant technology, including at least one domain selected from domains I, II, and III of serum albumin but having a different structure from that of native albumin; and a method of producing the protein. The protein has an enhanced functional activity or activities selected from among various functional activities or serum albumin including antibacterial activity, antioxidative effect, inflammation inhibitory effect, in vivo substance transporting action, and enzymatic activity.

Description

BACKGROUND OF THE INVENTION[0001]The present invention relates to a protein containing a serum albumin domain. The present invention more specifically relates to a protein having an enhanced functional activity or activities selected from among the various functional activities of the domains of serum albumin including antibacterial activity, antioxidative effect, inflammation inhibitory effect, in vivo substance transporting action, and enzymatic activity. Such a protein is provided by producing proteins of various combinations of serum albumin domains by gene recombinant technology.BACKGROUND ART[0002]Human serum albumin (HSA) is a main protein found in the serum of an adult, is produced in the liver, and has a function as a carrier for transporting various serum molecules. In addition, the albumin has an important role in maintaining at a normal level a plasma colloid osmotic pressure caused by a solute (colloid) which cannot pass through pores of a capillary vessel, to maintain ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(United States)
IPC IPC(8): C07K1/00C12N15/09A61K38/00A61P39/06C07K14/765C12N1/15C12N1/19C12N1/21C12N5/10C12P21/02
CPCC07K14/765A61P39/06
Inventor OTAGIRI, MASAKIKIDA, YOSHINORIKATAYAMA, NAOHISAKAI, TOSHIYA
Owner NIPRO CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com