Protein containing serum albumin domain

Abstract

A protein produced by gene recombinant technology, including at least one domain selected from domains I, II, and III of serum albumin but having a different structure from that of native albumin; and a method of producing the protein. The protein has an enhanced functional activity or activities selected from among various functional activities or serum albumin including antibacterial activity, antioxidative effect, inflammation inhibitory effect, in vivo substance transporting action, and enzymatic activity.

Description

BACKGROUND OF THE INVENTION[0001]The present invention relates to a protein containing a serum albumin domain. The present invention more specifically relates to a protein having an enhanced functional activity or activities selected from among the various functional activities of the domains of serum albumin including antibacterial activity, antioxidative effect, inflammation inhibitory effect, in vivo substance transporting action, and enzymatic activity. Such a protein is provided by producing proteins of various combinations of serum albumin domains by gene recombinant technology.BACKGROUND ART[0002]Human serum albumin (HSA) is a main protein found in the serum of an adult, is produced in the liver, and has a function as a carrier for transporting various serum molecules. In addition, the albumin has an important role in maintaining at a normal level a plasma colloid osmotic pressure caused by a solute (colloid) which cannot pass through pores of a capillary vessel, to maintain ...

AI Technical Summary

Benefits of technology

[0007]The inventors of the present invention have established an expression system, which is capable of preparing a DNA sequence encoding each domain of serum albumin composed of domains I, II, and III, transforming a host cell using a vector containing such a DNA sequence, and producing a protein containing the serum albumin domain in the host cell. Further, the inventors of the present invention have conducted a functional analysis on each domain. As a result, the inventors of the present invention have found that a specific domain is responsible for most of specific biological activities of albumin. In addition, the inventors of the present invention have found that a protein having various enhanced functional activities of albumin can be provided by producing a protein containing a large amount of the specific domain by gene recombinant technology, and have completed the present invention. In particular, the inventors of the present invention have found out that albumin domain I has high antioxidative ability while showing low enzyme activity. Therefore, a protein containing domain I can provide an enhanced antioxidative effect.

[0008]As a result of the investigation of the binding property of three site I markers (subsite Ia, Ib, Ic), only subsite Ic ligand shows binding with domain II though the binding strength is weak. The site II drugs show a binding property with domain II in a ratio of 60 to 80% compared to the wild type albumin. However an esterase-like activity is shown to be remarkably high in the domain III but significantly less than a wild type albumin. An enolase-like activity is not shown in any domain at pH 7.2 but is shown in the domain II at pH 9.2. Further, it is interesting that an antioxidative effect of domain I is substantially similar to that of the wild type albumin. Moreover, each domain reflects a molecular weight to show a large clearance in the kidney. As domain I shows high antioxidant property and low enzymatic activity, it is expected that a protein genetically modified to contain albumin domain I will be applied to a drug delivery system. A protein containing a plurality of amino acid sequences corresponding to domain I will show enhanced antioxidant property.

Problems solved by technology

Meanwhile, pharmaceutical preparations using albumin derived from the blood may be contaminated with an unknown virus.

Thus, the use thereof for the human body has caused safety problems.

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