Method for refining papain
A technology of papain and papain, which is applied in the field of papain preparation, can solve the problems of unfavorable industrial production of refined papain, restriction of papain application, complex production process, etc., and achieve the reduction of enzyme activity and enzyme quality. Loss, the effect of high purity
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Example Embodiment
[0025] Example 1
[0026] The manufacturing method of refined papain in this embodiment includes the following steps: papaya pulp→pretreatment→milling→heat exchange→two-stage centrifugation→microfiltration→ultrafiltration→vacuum freeze drying→modulation, pulverization, and finished product. The specific operations are as follows: harvesting fresh papaya pulp, adding 0.5% antioxidant and 0.5% heavy metal chelating agent, and fully stirring for 20 minutes; pouring the pretreated papaya pulp into a colloid mill and fully grinding to obtain papain enzyme feed solution ; The temperature of papain feed liquid is rapidly reduced to 8°C through the plate heat exchanger, and in the subsequent extraction and separation process, the feed liquid is maintained at this temperature range through the cold water circulation system; The clarified papain clarified feed solution is obtained through continuous and continuous separation; the papain clarified feed solution is passed through a microf...
Example Embodiment
[0027] Example 2
[0028] The manufacturing method of refined papain in this embodiment includes the following steps: papaya pulp→pretreatment→milling→heat exchange→two-stage centrifugation→microfiltration→ultrafiltration→vacuum freeze drying→modulation, pulverization, and finished product. The specific operations are as follows: harvesting fresh papaya pulp, adding 1.0% antioxidant and 1.0% heavy metal chelating agent, and fully stirring for 30 minutes; pouring the pretreated papaya pulp into a colloid mill and fully grinding to obtain papain enzyme feed solution ; The papain enzyme feed liquid is rapidly lowered to 12°C through the plate heat exchanger, and in the subsequent extraction and separation process, the feed liquid is maintained at this temperature range through the cold water circulation system; The clarified papain feed solution is obtained by continuous and continuous separation; the papain clarified feed solution is passed through a microfiltration membrane mod...
Example Embodiment
[0029] Example 3
[0030] The manufacturing method of refined papain in this embodiment includes the following steps: papaya pulp→pretreatment→milling→heat exchange→two-stage centrifugation→microfiltration→ultrafiltration→vacuum freeze drying→modulation, pulverization, and finished product. The specific operations are as follows: harvesting fresh papaya pulp, adding 0.6% antioxidants and 0.6% heavy metal chelating agent, and fully stirring for 25 minutes; pouring the pretreated papaya pulp into a colloid mill and fully grinding to obtain papain enzyme feed solution ;The temperature of papain liquid is rapidly reduced to 10 ℃ through the plate heat exchanger, and in the subsequent extraction and separation process, the liquid is maintained in this temperature range through the cold water circulation system; The clarified papain feed solution is obtained by continuous and continuous separation; the papain clarified feed solution is passed through a microfiltration membrane modul...
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