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siRNA designed based on the conserved olfactory receptor Orco gene of sitobion avenae and its application

A wheat long tube aphid, gene technology, applied in DNA/RNA fragment, application, genetic engineering and other directions, can solve problems such as reducing control effect

Active Publication Date: 2018-11-23
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the effective implementation of the above measures depends on the cooperation of environmental conditions, wind, rain, low temperature, etc. will reduce the control effect

Method used

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  • siRNA designed based on the conserved olfactory receptor Orco gene of sitobion avenae and its application
  • siRNA designed based on the conserved olfactory receptor Orco gene of sitobion avenae and its application
  • siRNA designed based on the conserved olfactory receptor Orco gene of sitobion avenae and its application

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Embodiment Construction

[0022] Below in conjunction with example the present invention is described in further detail.

[0023] 1. Materials and methods

[0024] 1.1 Insects to be tested

[0025] The experimental population of the monosexual line of Aphid sativa was originally derived from the wild population in Langfang, multiplied by a single head, and fed to wheat plants. The wheat variety is Zhongmai 175. Select low-density (10 wheat seedlings / pot, average 1 head / plant) to feed more than 3 generations of Aphid aphid adults and their offspring as test insects. The breeding temperature was maintained at 22±1°C, the relative humidity was 75%, and the photoperiod was 16h︰8h.

[0026] 1.2 siRNA sequence design

[0027]Using the cDNA of SaveOrco (Genbank accession number: GQ275379) as a template, siRNA was designed and synthesized according to the principle of siRNA sequence design, named SiOrco8, and the scrambled siRNA was used as a negative control, named NegOrco8. See Table 1 for sequence conte...

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Abstract

The invention relates to siRNA designed based on the conservative olfactory receptor Orco gene of sitobion avenae and an application thereof. In the invention, siOrco8 is fed to interfere with the transcription of the SaveOrco gene. Real-time fluorescent quantitative PCR study indicates that the expression of the SaveOrco gene is obviously inhibited. The olfactory behavior reaction detection result shows that the responsivity of aphids with expression loss of SaveOrco to cis-3-hexenol, methyl salicylate and EBF is remarkably reduced. In an experiment of EBF inducing wing dimorphism of sitobion avenae, the aphids treated with siRNA stop induction of EBF, normal chemical communication of the aphids is disturbed, and the stress-inducible wing bud generation is also stopped. Therefore, the conservative sequence of the Orco gene can be used for effectively preventing aphids from quickly spreading and doing harm through the siRNA-mediated RNAi technology so as to control aphids.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to the siRNA sequence of the olfactory receptor gene SaveOrco of the long tube aphid Sitobion avenae and its application in the field of aphid prevention and control. Background technique [0002] Aphids have a wide variety of species and a wide range of hosts, many of which are important agricultural pests. It feeds on thorns and feeds on the phloem juice of host plants, and causes serious losses in the yield and quality of host crops by plundering host nutrients, carrying and spreading plant viruses, and excreting honeydew. Insects mainly use their sense of smell to understand their surrounding habitats, and aphids are no exception, such as the location of host plants at close range [Webster BEN (2012) The role of olfaction in aphid host location. Physiological Entomology 37:10-18.] or the recognition of pheromones [Mustaparta H (1990) Chemical information processing in the olf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A01N57/16A01P7/04
CPCA01N57/16C12N15/113C12N2310/14
Inventor 范佳陈巨莲徐庆宣孙京瑞
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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