siRNA designed based on the conserved sequence of the olfactory receptor SaveOrco of sitobion avenae and its application
A technology of wheat long tube aphid and wheat aphid, which is applied in the field of biology, siRNA sequence and its prevention and control of aphids, and can solve the problems of reducing the control effect
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[0022] Below in conjunction with example the present invention is described in further detail.
[0023] 1. Materials and methods
[0024] 1.1 Insects to be tested
[0025] The experimental population of the monosexual line of Aphid sativa was originally derived from the wild population in Langfang, multiplied by a single head, and fed to wheat plants. The wheat variety is Zhongmai 175. Select low-density (10 wheat seedlings / pot, average 1 head / plant) to feed more than 3 generations of Aphid aphid adults and their offspring as test insects. The breeding temperature was maintained at 22±1°C, the relative humidity was 75%, and the photoperiod was 16h︰8h.
[0026] 1.2 siRNA sequence design
[0027]Using the cDNA of SaveOrco (Genbank accession number: GQ275379) as a template, siRNA was designed and synthesized according to the siRNA sequence design principle, named SiOrco9, and the scrambled siRNA was used as a negative control, named NegOrco9. See Table 1 for sequence content....
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