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Method for obtaining young winged aphids of macrosiphum avenae in rapid and directional mode

A technology of tube aphid and age, which is applied in the field of rapid and directional acquisition of young winged nymphs of tube aphid, can solve the problem of inability to compare gene differential expression changes in the critical period of wing type differentiation, inability to distinguish winged or wingless, etc. problems, to achieve the effect of convenient operation and broad application prospects

Active Publication Date: 2017-06-20
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

However, the current research has found that the pseudo-embryos of A. sativa, the 1st and 2nd instar nymphs cannot be distinguished from winged or wingless by appearance. Swell and grow wing buds to distinguish between winged and wingless aphids
Therefore, current studies on the molecular mechanism of aphid wing type differentiation are all aimed at gene expression analysis after aphid wing type differentiation, and it is impossible to compare the differential expression changes of genes in the critical period of wing type differentiation

Method used

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  • Method for obtaining young winged aphids of macrosiphum avenae in rapid and directional mode
  • Method for obtaining young winged aphids of macrosiphum avenae in rapid and directional mode
  • Method for obtaining young winged aphids of macrosiphum avenae in rapid and directional mode

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Embodiment

[0024] S1: Select 10 adult aphids of the wingless wheat nymph, culture them individually in a petri dish, and multiply them for three generations, and select 3 healthy nymphs for each generation, and continue to culture them separately;

[0025] S2: Raise the fourth-generation nymphs individually until the third instar, and then take 10 heads and place them in the same airtight culture dish with a diameter of 120 mm;

[0026] S3: Add 2 μL of E-β-farnesene with a concentration of 500 ng / μL to the airtight culture dish, the solvent of E-β-farnesene solution is n-hexane, and then add it once every 2 hours, adding 5 times as a cycle ; Repeat S301 step 4 cycles (i.e. handle 5 days altogether); Wherein, the time point of adding E-β-farnesene solution for the first time in each cycle is the time of adding E-β-farnesene solution for the first time with the first cycle point, remove all nymphs produced by adult aphids during this period;

[0027] After 5 days of adding the E-β-farnese...

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Abstract

The invention relates to a method for obtaining young winged aphids of macrosiphum avenae in a rapid and directional mode. The method comprises the steps that S1, adult wingless aphids of macrosiphum avenae are raised in a single mode, and propagation of three generation is carried out; S2, aphids of the fourth generation are raised to third instar in a single mode, and a plurality of raised aphids of the fourth generation are put into a same culture device; S3, E-beta-farnesene solutions are added into the culture device repeatedly, and after the E-beta-farnesene solutions are added, young winged pseudo embryos and / or aphids of macrosiphum avenae are collected. According to the method for obtaining the young winged aphids of macrosiphum avenae in a directional mode, pseudo embryos which can develop winged macrosiphum avenae, first instar aphids and second instar aphids which can develop winged macrosiphum avenae, third instar aphids of winged macrosiphum avenae, fourth instar aphids of winged macrosiphum avenae and adult aphids of winged macrosiphum avenae are obtained in a directional mode, and the basis of the aphid gene expression study of the pseudo embryo period, first instar period and second instar period is provided; the method is convenient, simple and easy to operate, and wide in application prospect.

Description

technical field [0001] The invention relates to the technical field of insect biology, in particular to a method for rapidly and directionally obtaining young winged nymphs of the long tube aphid. Background technique [0002] The wheat aphid has a typical dispersal polymorphism. Under the regulation of external environmental factors, a single genetic type of wheat aphid can produce two different biotypes during the development process: winged and wingless. Like other winged dimorphic insects, winged wheat aphids can carry out long-distance migratory diffusion. After reaching the destination, they can form wingless aphids for reproduction and expansion, and cause damage to large-scale breeding offspring. Developmental plasticity is an important survival strategy formed by aphids to adapt to the changing ecological environment during the long-term evolution process. If the generation of winged aphids can be effectively prevented, the food loss caused by wheat aphids will be s...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 李祥瑞张方梅张云慧魏长平程登发朱勋
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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