30 results about "Beta-farnesene" patented technology

The invention discloses saccharomyces cerevisiae capable of auto-synthesizing terpenoid substances and applications thereof, belonging to the field of fermentation engineering. The saccharomyces cerevisiae capable of auto-synthesizing various terpenoid substances denovo is screened from fermented grains of white wine. The stain is preserved in the general microbiology centre in China on September 4, 2013, and the preservation number is CGMCC No.8130. The strain is subjected to liquid-state or solid-state fermented cultivation so as to generate ten kinds of terpenoid substances including linalool, alpha-terpineol, citronellol, myrcenol, nerolidol, alpha-dragosantol, farnesol, alpha-farnesene, beta-farnesene and bisabolene. The cerevisiae strain is added in the wine making process and can improve the flavor of white wine and increase the nutritive value of white wine.

The invention constructs a beta-farnesene producing bacterium through genetic engineering, and the beta-farnesene producing bacterium is preserved in the China General Microbiological Culture Collection Center, CGMCC, wherein the preservation number is CGMCC No.16709. The beta-farnesene producing bacterium can realize effective accumulation of beta-farnesene, the yield of the beta-farnesene is ashigh as 250mg / L, and the beta-farnesene producing bacterium has industrial prospects.

The invention discloses a (trans)-beta-farnesene analogue containing a jasmonic acid base group, as well as preparation and application of the (trans)-beta-farnesene analogue belonging to the field of organic chemistry synthesis. The preparation of the (trans)-beta-farnesene analogue comprises the following steps of adding alkali and a condensing agent to an organic solvent, and in the organic solvent, enabling compounds as shown in a formula III or a formula V to be subjected to a condensation reaction so as to obtain the (trans)-beta-farnesene analogue containing the jasmonic acid base group, as shown in a formula I or a formula II. A synthetic method is simple to operate, raw materials are easy to obtain, harmful by-products are not produced, and the obtained products are stabler than trans-beta-farnesene. The (trans)-beta-farnesene analogue containing the jasmonic acid base group shows multiple bioactivity; the (trans)-beta-farnesene analogue with high dosage has good killing activity on budworms, and the (trans)-beta-farnesene analogue with low dosage has expelling activity on the budworms, so that the (trans)-beta-farnesene analogue has a high application development value.

The invention provides a thrip attractant and insecticide combined trapping and killing method. An attractant is prepared by taking liquid paraffin as a solvent and mixing 100 [mu] g / ml of methyl nicotinate, trans-beta farnesene, caprylaldehyde, 3-carene, isoeugenol and beta-phenethyl alcohol with the same mass. The attractant disclosed by the invention contains specific host plant source volatilesubstances, is diverse in variety, wide in action target sites and strong in complementarity; a compound with a relatively strong trapping effect on thrips is prepared according to a certain proportion; thrips are trapped on an insect sticking color plate, and meanwhile, thrip injurious insecticide high-efficiency and low-residue pesticide is sprayed on the insect sticking color plate; the thripsare killed by utilizing the toxicity of the insecticide, so that the thrips are thoroughly killed, and secondary escape of thethrips is prevented from causing harm. The attractant disclosed by the invention has the characteristics of low cost, environmental protection, high efficiency, low possibility of generating drug resistance and the like; and the trapping substance is derived from plants, is safe and reliable, can be widely applied to vegetables, fruit trees and other crops, and has wide application prospects.

The invention provides an empoasca vitis attractant based on tropic ageratum herb volatile matter. The attractant is a mixture prepared from four active components including cis-beta-farnesene, alpha-cubebene, caryophyllene and humulene in the concentration being 10<-1> mg / ml-10<-5> mg / ml in the weight ratio being 1: 1: 1: 1. The attractant takes the artificially synthesized volatile matter as the main material, has the obvious attraction effect on empoasca vitis and has the characteristics of being convenient to use, pollution-free and the like.

The invention discloses an attractant for Anoplophora glabripennis adults. The attractant comprises (E)-beta-Farnesene or Farnesene containing six isomers of the (E)-beta-Farnesene, (Z)-beta-farnesene, (E,E)-alpha-Farnesene, (Z,Z)-alpha-Farnesene, (E,Z)-alpha-Farnesene and (Z,E)-alpha-farnesene; the attractant can be used for attracting female and male Anoplophora glabripennis adults; besides, the attractant can be used for attracting female and male adults in field, and provides a practical nuisanceless monitoring and control technical means for monitoring the occurrence period and the occurrence amount of the adults or large-scale trap and kill in forest.

The invention discloses application of a rice terpene synthetase TPS46 gene to prevention and controll of pink rice borers and also includes a pink rice borer attractant. The pink rice borer attractant contains the rice terpene synthetase TPS46 gene or transgenic rice thereof after overexpression. The pink rice borer attractant also contains a mixture of (E)-beta-farnesene, methylsalicylic acid, (E)-alpha-bergamotene, (E)-beta-caryophyllene, alpha-humuleno, linalool, alpha-bisabolene and limonene. The rice TPS46 gene which makes overexpression on rice plants and can remarkably lure spontaneousgeneration of the pink rice borers is authenticated for the first time; and field tests show that for rice with overexpression of the TPS46 gene, the harms of the pink rice borers to non-transgenic control rice can be remarkably lowered, and field attraction and comprehensive prevention and control of the pink rice borers are facilitated.

The invention discloses a quality control method for dried ginger. The method comprises the following steps of: determining the content of 4 ginger phenolic chemical components including 6-gingerol, 8-gingerol, 6-shogaol and 10 gingerol, and 2 volatile oil chemical components including alpha-curcumene and (E)-beta-farnesene in the dried ginger medicinal materials by adopting an HPLC-PDA method; and taking the total amount of the ginger phenolic components, the total amount of the volatile oil components, or the total amount of the ginger phenolic components and the volatile oil components as an evaluation index, which is used for formulating a standard for evaluating the quality of the dried ginger medicinal material and controlling the quality. The method is capable of comprehensively, objectively and effectively evaluating the quality of the dried ginger medicinal material by simultaneously detecting two types of the active ingredients of the dried ginger medicinal material and analyzing the fingerprint spectrum and comparing with a single analysis of one or a class of chemical components, and is used for the quality control thereof, and the method is simple and convenient to operate, good in stability and easy to be popularized.

The invention discloses an efficient preparation method and application of poly beta farnesene with ultra-high molecular weight. The invention belongs to the field of polymer synthesis. The inventionaims to solve the technical problems of low molecular weight of poly beta farnesene obtained by existing methods and higher cost of adopted rare earth metal reactants. The method comprises the following steps: adding a solvent, a main catalyst, a cocatalyst and a beta-farnesene monomer into a reactor according to any sequence under the protection of inert gas, performing polymerizing for 10-240 minutes at 0-100 DEG C while stirring, adding a quenching agent and an anti-aging agent into the reaction system for quenching reaction, repeatedly performing washing with ethanol, and carrying out vacuum drying to obtain the poly beta-farnesene . The main catalyst is a bipyridine iron complex. The microstructure of the obtained poly beta-farnesene is composed of 3,4-poly beta-farnesene with the molar fraction of 50%-70% and 1,4-poly beta-farnesene with the molar fraction of 30%-50%, the number-average molecular weight of the obtained poly beta-farnesene ranges from 8.0 x 10<5> g / mol to 1.7 x 10<6> g / mol, and the molecular weight distribution ranges from 1.5 to 3.5.

The invention discloses EBF ((E)-beta-Farnesene) analogues containing pyrazole-carboxamide, and a preparation method and application thereof. The analogues have a structural general formula shown in formula I, wherein R1 is methyl, n-propyl, isopropyl or phenyl; R2 is t-butyl, phenyl or 4-nitrophenyl; R3 is H or methyl; and X is H or Cl. The preparation method of the compounds shown in formula I provided by the invention is simple to operate and low in cost. Compared with EBF, the compounds shown in formula I have higher stability, and part of compounds shown in I1, I11 and I13 have the aphidkilling rate above 90%, thus having potential application value in control of aphid.

The invention relates to a (cis)-beta-farnesene analog containing a 1,2,3-thiadiazole group, a preparation method and applications thereof, and belongs to the field of organic synthesis. The 1,2,3-thiadiazole group containing (cis)-beta-farnesene analogs with different substituents are obtained through the condensation reactions among geraniol, geranylamine / substituted geranylamine, and 4-R1-1,2,3-thiadiazole-5-formic acid; and then the analogs are separated and purified by silica gel column chromatography to obtain a compound represented by the formula I. The provided synthesis method has the advantages of simple operation, easily-available raw materials, and difficulty in generating harmful byproducts, and moreover, the target compound is more stable than cis-(beta)-farnesene. The provided analog has multiple bioactivities, analog with a high dosage can kill aphid, analog with a low dosage can repel aphid, at the same time the analog can prevent tobacco mosaic virus and kill bacteria, and thus the analog is worth to develop.

The invention discloses an anti-beta-farnesene analog containing guanidine or nitro-ethylene, belonging to the field of organic compound synthesis. The compound is in a structure in a general formula (1) and is obtained by making raw materials, such as S-methyl-N-nitro phthalic acyl isothiourea, cyano imine dimethyl dithiocarbamate or 1,1-dimethyl sulfur-2-nitroethylene react with different amines under alkaline condition. The compound has favorable aphid killing chemical activity. Compared with anti-beta-farnesene, the compound has high activity and good stability and can be practically applied to agricultural production.

The invention discloses a sex pheromone synergist for preventing and controlling Grapholitha molesta, and a preparation method thereof, wherein the synergist comprises beta-ocimene, (E)-beta-farneseneand 2,6-di-tert-butyl-4-methylphenol according to a ratio of 1:0.2:0.05. The preparation method comprises: mixing beta-ocimene, (E)-beta-farnesene and 2,6-di-tert-butyl-4-methylphenol according to the mass ratio, and adding the three prepared solutions to a polyvinyl chloride or polyethylene pipe by a peristaltic pump; sealing the filled polyvinyl chloride or polyethylene pipe every 3-5 cm by a clamp type sealing machine, and cutting; and placing the cut polyvinyl chloride or polyethylene pipe in a refrigerator so as to be used for spare. According to the present invention, the synergist cangreatly increase the trapping amount of the Grapholitha molesta sex pheromone in the field, can further prolong the sustained release time of the Grapholitha molesta sex pheromone, and further has characteristics of no toxicity, no harm to natural enemies, no residue and easy ecological environment protection.

The invention discloses an application of rice terpene synthetase tps46 gene and metabolite thereof in controlling Chilo suppressalis. The invention comprises tps46 transgenic overexpressed rice withobvious toxic effect on Chilo suppressalis larvae and rice secondary metabolite single product regulated by tps46 with obvious lethal effect. The compound having significant toxic effect on Chilo suppressalis larvae comprises methyl salicylate, ((E)-Beta-Farnesene, Linalool, (E)- Beta-Caryophyllene, alpha-humulene and limonene and mixtures thereof. The invention also comprises tps46 transgenic overexpressed rice and tps46-regulated rice secondary metabolite alpha-humulene and (E)-Beta-Farnesene, which can significantly attract Apanteles chilonis. Apanteles chilonis has a strong parasitic effect on Chilo suppressalis larvae, and can control Chilo suppressalis effectively and greenly.

The invention discloses a plant aphid resistance realization method based on a 35S promoter. The method comprises the following steps: cloning to obtain EbetaF synthetase genes EbetaFS from peppermintMenthaXpiperita, constructing an expression vector, transferring the vector into agrobacterium, infecting wheat embryos with the agrobacterium, catalyzing FPP to synthesize trans-beta-farnesene in wheat, synthesizing aphid alarm toxin trans-beta-farnesene in the wheat, and transporting to transgenic wheat to release the trans-beta-farnesene, thereby achieving the effect of dispelling aphids.

The invention discloses a (trans)-beta-farnesene analogue containing a jasmonic acid base group, as well as preparation and application of the (trans)-beta-farnesene analogue belonging to the field of organic chemistry synthesis. The preparation of the (trans)-beta-farnesene analogue comprises the following steps of adding alkali and a condensing agent to an organic solvent, and in the organic solvent, enabling compounds as shown in a formula III or a formula V to be subjected to a condensation reaction so as to obtain the (trans)-beta-farnesene analogue containing the jasmonic acid base group, as shown in a formula I or a formula II. A synthetic method is simple to operate, raw materials are easy to obtain, harmful by-products are not produced, and the obtained products are stabler than trans-beta-farnesene. The (trans)-beta-farnesene analogue containing the jasmonic acid base group shows multiple bioactivity; the (trans)-beta-farnesene analogue with high dosage has good killing activity on budworms, and the (trans)-beta-farnesene analogue with low dosage has expelling activity on the budworms, so that the (trans)-beta-farnesene analogue has a high application development value.

A reduced pressure fluidizing catalytic reaction system for preparing (trans)-beta-farnesene from nerolidol is composed of a feeder, a fluidized-bed reactor, a temp controller, a product collector, a pressure reducing unit and a condenser. It has features of the continuous feed raw material and high reaction speed.

The invention discloses EBF ((E)-beta-Farnesene) analogues containing pyrazole-carboxamide, and a preparation method and application thereof. The analogues have a structural general formula shown in formula I, wherein R1 is methyl, n-propyl, isopropyl or phenyl; R2 is t-butyl, phenyl or 4-nitrophenyl; R3 is H or methyl; and X is H or Cl. The preparation method of the compounds shown in formula I provided by the invention is simple to operate and low in cost. Compared with EBF, the compounds shown in formula I have higher stability, and part of compounds shown in I1, I11 and I13 have the aphidkilling rate above 90%, thus having potential application value in control of aphid.

The invention discloses a method for combined prevention and treatment of aphis pests by using pheromone complex and ladybugs. An aphid alarm pheromone and a plant greenery odor are used as basic components and are prepared into a slow-release carrier by virtue of different proportions; the released E-beta-farnesene not only can directly avoid and keep away aphids but also can enable the aphids to generate uproar to improve the effect of discovery and eating of the aphids by the ladybugs; and the added greenery odor can appropriately adjust the stability of the release of the alarm pheromone and increase the release lasting period of the alarm pheromone so as to achieve an aim of avoiding, keeping away, catching and killing the aphids. A prevention and treatment test proves that by use of the method disclosed by the invention, the prevention and treatment effect, compared with that of separate use of the ladybugs or the pheromone for prevention and treatment, can be improved by 25-45%; and the method disclosed by the invention can reduce the usage amount of chemical pesticides to reduce environmental pollution and enable the aphids to generate drug resistance difficultly, thereby protecting biodiversity and natural enemies resources, and developing a new means of the pheromone in the field of prevention and treatment of pests.

The invention relates to a method for obtaining young winged aphids of macrosiphum avenae in a rapid and directional mode. The method comprises the steps that S1, adult wingless aphids of macrosiphum avenae are raised in a single mode, and propagation of three generation is carried out; S2, aphids of the fourth generation are raised to third instar in a single mode, and a plurality of raised aphids of the fourth generation are put into a same culture device; S3, E-beta-farnesene solutions are added into the culture device repeatedly, and after the E-beta-farnesene solutions are added, young winged pseudo embryos and / or aphids of macrosiphum avenae are collected. According to the method for obtaining the young winged aphids of macrosiphum avenae in a directional mode, pseudo embryos which can develop winged macrosiphum avenae, first instar aphids and second instar aphids which can develop winged macrosiphum avenae, third instar aphids of winged macrosiphum avenae, fourth instar aphids of winged macrosiphum avenae and adult aphids of winged macrosiphum avenae are obtained in a directional mode, and the basis of the aphid gene expression study of the pseudo embryo period, first instar period and second instar period is provided; the method is convenient, simple and easy to operate, and wide in application prospect.

The invention relates to a ubiquitin-1 promoter-based plant aphid resistance implementation method. An E-beta-farnesene synthase (EbetaFS) gene is cloned from peppermint by means of genetic engineering, and a ubiquitin-1 promoter is constructed for driving an EbetaF vector. An gene is transferred into wheat by using a gene gun transformation method; E-beta-farnesene is synthesized by catalyzing FPP in the wheat; the release of the E-beta-farnesene in transgenic wheat is realized by synthesizing an aphid alarm element E-beta-farnesene in the wheat, so that the aim of avoiding aphids is achieved.

The invention discloses a [trans]-β-farnesene analogue, a preparation method thereof and an application thereof. The [trans]-β-farnesene analog is a compound represented by formula I, wherein, in formula I, Y is O or S, and Z is O, S or NCN. The compound of the present invention has good chemical killing activity on aphids, especially at low concentrations, the aphidicidal activity of the compound of the present invention is better than that of the contrast agent thiacloprid, and compared with EBF, the compound of the present invention has a large molecular weight and stable Well, it can be practically applied in agricultural production.

The invention discloses an anti-beta-farnesene analog containing guanidine or nitro-ethylene, belonging to the field of organic compound synthesis. The compound is in a structure in a general formula (1) and is obtained by making raw materials, such as S-methyl-N-nitro phthalic acyl isothiourea, cyano imine dimethyl dithiocarbamate or 1,1-dimethyl sulfur-2-nitroethylene react with different amines under alkaline condition. The compound has favorable aphid killing chemical activity. Compared with anti-beta-farnesene, the compound has high activity and good stability and can be practically applied to agricultural production.

The invention relates to a method for producing farnesyl amines by reacting beta-farnesene with one or more amines in the presence of a transition metal catalyst of the10th subgroup, at a temperaturein the region of between 60-150 DEG C.

The invention relates to the technical field of biological fermentation, in particular to a method for extracting beta-farnesene from fermentation broth. The method comprises the step S1 of dual-phaseextraction fermentation, the step S2 of flocculation removal, the step S3 of water vapor vacuum distillation and the step S4 of oil-water separation. According to the method, the loss of a target product in the fermentation process is greatly reduced by adding an organic solvent in the fermentation stage for duplex fermentation. A flocculant is added to remove residual bacteria, proteins and small molecular impurities existing in the downstream product extraction process, and the solvent extraction process and the water vapor distillation process are coupled to effectively increase the yieldand the purity of the beta-farnesene. The method breaks through the bottleneck of separation and purification technology of high-purity beta-farnesene products, achieves continuous production, significantly reduces the energy consumption of production, especially improves the recycling rate of the organic solvent, reduces the production of organic wastewater and achieves clean production.

A reduced pressure fluidizing catalytic reaction system for preparing (trans)-beta-farnesene from nerolidol is composed of a feeder, a fluidized-bed reactor, a temp controller, a product collector, a pressure reducing unit and a condenser. It has features of the continuous feed raw material and high reaction speed.

The invention relates to a method for preparing beta-farnesene by fermentation of sugar sorghum stalks and sugarcane waste residues. The method comprises the following steps of: A, harvesting and squeezing mature and fresh sugar sorghum to obtain sugar sorghum juice; B, concentrating the sugar sorghum juice; C, uniformly mixing a sugar sorghum juice concentrated solution with the sugarcane waste residues which are by-products produced in a sugar refinery, and obtaining a mixed solution of the sugar sorghum and the sugarcane waste residues; D, taking the mixed solution of the sugar sorghum and the sugarcane waste residues as a carbon source of fermentation, and taking Saccharomyces cerevisiae of which the collection number is CICC No. 32475 as a strain to prepare a shake flask strain; E, inoculating the shake flask strain to a seed culture medium to be cultured; G, inoculating a seed culture fluid to a fermentation medium to be fermented for 30 to 50 hours, adding a flocculating agent to a fermentation broth, and obtaining a supernatant through siphoning after precipitation. According to the method for preparing beta-farnesene by the fermentation of the sugar sorghum stalks and the sugarcane waste residues disclosed by the invention, the adopted raw materials are low in cost, and the content of the beta-farnesene in the obtained supernatant is high, thereby being beneficial to reduction of the preparation cost of squalane derived from plants.

The invention provides natural rose essential oil which is characterized by comprising the following raw materials in parts by weight: 10-20 parts of citronellol, 10-20 parts of geraniol, 5-10 parts of eugenol, 0.1-0.3 part of camellia oil, 1-3 parts of michelia alba leaf oil, 1-3 parts of neroli oil, 1-3 parts of rose oil, 1-3 parts of jasmin oil, 0.5-1.5 parts of nerolin, 0.5-1.5 parts of nerol,0.5-1.5 parts of carvacrol, 1-2 parts of alpha-elemene, 1-2 parts of alpha-cedrene, 0.1-0.5 part of alpha-amorpha fruticosa alkene, 0.1-0.3 part of peanut oil, 0.1-0.2 part of beta-ionone, 0.1-0.2 part of beta-farnesene, 0.5-1 part of sunflower oil, 0.1-0.3 part of hexadecane, 0.1-0.3 part of octodecane, 0.1-0.3 part of tetracosane and 0.1-0.3 part of linalool. Through adoption of the technical scheme, the rose essential oil has the effects of being natural, mild and non-irritant.

The invention relates to the technical field of biological mutagenesis breeding, and in particular, relates to a compound mutagenesis breeding method of a mutant strain for highly yielding beta-farnesene. The method includes the steps: preparation of a bacterial suspension, plasma-ultraviolet compound mutagenesis, flat plate primary screening, shake flask re-screening and fermentation tank verification. According to the compound mutation breeding method of the mutant strain for highly yielding beta-farnesene, through plasma-ultraviolet compound mutagenesis, flat plate primary screening and shake flask re-screening are adopted, and finally, the fermentation unit of beta-farnesene of the mutant strain for highly yielding beta-farnesene in the fermentation tank is 169.10% higher than that ofa starting strain, and the mutant strain still has good hereditary stability through 8 times of consecutive passage.