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A kind of method of using pheromone compound and ladybug to jointly prevent and control aphid class pests

A compound and pheromone technology, applied in pest control, botany equipment and methods, chemicals for biological control, etc., to achieve the effects of improving control effects, protecting biodiversity and natural enemy resources, and preventing drug resistance

Active Publication Date: 2019-11-19
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, there is no relevant report on the application of aphid alarm pheromone EβF and green leaf odor to the natural enemy ladybug to control aphids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: indoor test

[0013] 1) Take 3 pots of cabbage plants grown indoors, labeled as No. 1, No. 2, and No. 3 respectively. Cover the cabbage plants with insect-proof nets and place them in the laboratory. Take 200 aphids raised indoors and inoculate them in No. 1. On the leaves of potted cabbage plants, a female ladybug was released on the leaves of No. 1 cabbage plant, and the results were investigated after 24 hours of feeding. The experiment was repeated three times, and the female ladybugs released each time must be of the same age and the individuals were not much different. Take the results of this test as CK1.

[0014] After investigating the number of aphids on No. 1, No. 2 and No. 3 cabbage plants, the results showed that the number of aphids on the cabbage leaves of No. 1 plant was 80 ± 9.8, the number of aphids on the leaves of No. ±2.3 heads.

Embodiment 2

[0015] Embodiment 2: indoor test

[0016] 1) Dissolve trans-β-farnesene, cis-3-hexenol and cis-3-hexene acetate in n-hexane according to the mass ratio of 4:2:1, and prepare a sustained-release carrier solution with a certain concentration , and each added 0.5 mg of antioxidant 2,6-di-tert-butyl-p-cresol and ultraviolet light absorber 2-hydroxy-4-methoxybenzophenone, sealed in a polyvinyl chloride bag, and placed in 0 Store in -4°C refrigerator for later use.

[0017] 2) Take 3 pots of cabbage plants planted indoors, labeled as No. 1, No. 2, and No. 3 respectively. Cover the cabbage plants with insect-proof nets and place them in the laboratory. Take 200 aphids raised indoors and inoculate them in No. 1. On the leaves of potted cabbage plants, a female ladybug was released on the leaves of No. 1 cabbage plants, and at the same time, the compound slow-release carrier containing alarmin and green leaf odor was suspended at 3 cm above the cabbage plants, and the results were inv...

Embodiment 3

[0019] Embodiment 3: indoor test

[0020] 1) Dissolve trans-β-farnesene, cis-3-hexenol and cis-3-hexene acetate in n-hexane according to the mass ratio of 5:3:2, and prepare a sustained-release carrier solution with a certain concentration , and each added 0.5 mg of antioxidant 2,6-di-tert-butyl-p-cresol and ultraviolet light absorber 2-hydroxy-4-methoxybenzophenone, sealed in a polyvinyl chloride bag, and placed in 0 Store in -4°C refrigerator for later use.

[0021] 2) Take 3 pots of cabbage plants planted indoors, labeled as No. 1, No. 2, and No. 3 respectively. Cover the cabbage plants with insect-proof nets and place them in the laboratory. Take 200 aphids raised indoors and inoculate them in No. 1. On the leaves of potted cabbage plants, a female ladybug was released on the leaves of No. 1 cabbage plants, and at the same time, the compound slow-release carrier containing alarmin and green leaf odor was suspended at 3 cm above the cabbage plants, and the results were inv...

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PUM

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Abstract

The invention discloses a method for combined prevention and treatment of aphis pests by using pheromone complex and ladybugs. An aphid alarm pheromone and a plant greenery odor are used as basic components and are prepared into a slow-release carrier by virtue of different proportions; the released E-beta-farnesene not only can directly avoid and keep away aphids but also can enable the aphids to generate uproar to improve the effect of discovery and eating of the aphids by the ladybugs; and the added greenery odor can appropriately adjust the stability of the release of the alarm pheromone and increase the release lasting period of the alarm pheromone so as to achieve an aim of avoiding, keeping away, catching and killing the aphids. A prevention and treatment test proves that by use of the method disclosed by the invention, the prevention and treatment effect, compared with that of separate use of the ladybugs or the pheromone for prevention and treatment, can be improved by 25-45%; and the method disclosed by the invention can reduce the usage amount of chemical pesticides to reduce environmental pollution and enable the aphids to generate drug resistance difficultly, thereby protecting biodiversity and natural enemies resources, and developing a new means of the pheromone in the field of prevention and treatment of pests.

Description

technical field [0001] The invention belongs to the technical field of agricultural pest control, and in particular relates to a method for jointly controlling aphid pests by utilizing a pheromone compound and ladybugs. Background technique [0002] Aphids, also known as greasy insects and honey insects, are a class of herbivorous insects, including all members of the Aphidoidea (also known as Aphidoidea). There are about 4,400 species of aphids that have been discovered in 10 families, most of which belong to the family Aphididae. Aphids are also one of the most destructive pests on earth. About 250 of them are serious pests for agriculture, forestry and horticulture. Aphids have strong reproduction and adaptability, and conventional chemical control methods are difficult to achieve a radical cure effect, and the extensive use of chemical pesticides has made agricultural pests generally resistant to pesticides. How to effectively control its damage while reducing the env...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01M1/02A01N37/02A01N31/02A01N27/00A01N25/02A01P19/00
CPCA01G13/00A01M1/02A01N25/02A01N27/00A01N31/02A01N37/02A01N2300/00
Inventor 魏辉田厚军陈艺欣陈勇林硕张艳璇赵建伟
Owner INST OF PLANT PROTECTION FAAS
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